Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Dr, Fi, Gt, Ze, Po, Bv, Pm, Rb, RM, Xp
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 114
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tubulin alpha-1B Antibody (114) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Tubulin alpha-1B Antibody (114) - Azide and BSA Free (NBP2-43837) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Chimpanzee (99%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Tubulin alpha-1B Antibody (114) - Azide and BSA Free

• alpha tubulin
• Alpha-tubulin ubiquitous
• K-ALPHA-1
• tubulin alpha-1B chain
• Tubulin alpha-ubiquitous chain
• Tubulin K-alpha-1
• tubulin, alpha 1b
• tubulin, alpha, ubiquitous

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA1B