beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

Images

 
Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse ...read more
beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours ...read more
beta ‑III Tubulin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 8 ...read more
beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes ...read more
beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 ...read more
Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
TuJ-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific Summary

Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 8 Reviews rated 4.6
using
MAB1195 in the following applications:

Publications
Read Publications using
MAB1195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-III Tubulin Antibody (MAB1195)(148)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Amphibian, Avian - Chicken, Avian - Quail, Axolotl, Axolotl - Ambystoma mexicanum, Chicken, Porcine, Salamander, Xenograft.

We have publications tested in 8 applications: Flow Cytometry, HSI Microscopy, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(4)
HSI Microscopy
(1)
ICC
(78)
IHC
(27)
IHC-Fr
(19)
IHC-P
(5)
Immunoprecipitation
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(41)
Mouse
(72)
Rat
(21)
Amphibian
(1)
Avian - Chicken
(1)
Avian - Quail
(1)
Axolotl
(2)
Axolotl - Ambystoma mexicanum
(1)
Chicken
(2)
Porcine
(2)
Salamander
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 148. Show All 148 Publications.
Publications using MAB1195 Applications Species
DZ Hazelbaker, A Beccard, G Angelini, P Mazzucato, A Messana, D Lam, K Eggan, LE Barrett A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells Sci Rep, 2020;10(1):635. 2020 [PMID: 31959800] (ICC, Human) ICC Human
AL Green, J DeSisto, P Flannery, R Lemma, A Knox, M Lemieux, B Sanford, R O'Rourke, S Ramkissoon, K Jones, J Perry, X Hui, E Moroze, I Balakrishn, AF O'Neill, K Dunn, D DeRyckere, E Danis, A Safadi, A Gilani, B Hubbell-En, Z Nuss, JMM Levy, N Serkova, S Venkataram, DK Graham, N Foreman, K Ligon, K Jones, AL Kung, R Vibhakar BPTF regulates growth of adult and pediatric high-grade glioma through the MYC pathway Oncogene, 2019;0(0):. 2019 [PMID: 31844250] (ICC, Mouse) ICC Mouse
NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, D Steinemann, G Göhring, R König Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual Stem Cell Res, 2019;42(0):101679. 2019 [PMID: 31837633] (ICC, Human) ICC Human
NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, V des Portes, D Germanaud, D Steinemann, G Göhring, R König Induced pluripotent stem cells (iPSCs) derived from a renpenning syndrome patient with c.459_462delAGAG mutation in PQBP1 (PEIi001-A) Stem Cell Res, 2019;41(0):101592. 2019 [PMID: 31698189] (ICC, Human) ICC Human
D Fernández-, FA Krapacher, A Andersson, CF Ibáñez, L Kisiswa MAG induces apoptosis in cerebellar granule neurons through p75NTR demarcating granule layer/white matter boundary Cell Death Dis, 2019;10(10):732. 2019 [PMID: 31570696] (ICC, Mouse) ICC Mouse
A Philiastid, JM Ribes, DC Yip, C Schmidt, I Benilova, PC Klöhn A New Cell Model for Investigating Prion Strain Selection and Adaptation Viruses, 2019;11(10):. 2019 [PMID: 31546723] (IHC, Mouse) IHC Mouse
MA Skylar-Sco, SGM Uzel, LL Nam, JH Ahrens, RL Truby, S Damaraju, JA Lewis Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels Sci Adv, 2019;5(9):eaaw2459. 2019 [PMID: 31523707] (IHC, Human) IHC Human
Y Men, J Yelick, S Jin, Y Tian, MSR Chiang, H Higashimor, E Brown, R Jarvis, Y Yang Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS Nat Commun, 2019;10(1):4136. 2019 [PMID: 31515491] (Western Blot, Mouse) Western Blot Mouse
O Uckermann, J Hirsch, R Galli, J Bendig, R Later, E Koch, G Schackert, G Steiner, E Tanaka, M Kirsch Label-free Imaging of Tissue Architecture during Axolotl Peripheral Nerve Regeneration in Comparison to Functional Recovery Sci Rep, 2019;9(1):12641. 2019 [PMID: 31477751] (IHC-Fr, Salamander) IHC-Fr Salamander
J Jin, P Ravindran, D Di Meo, AW Püschel Igf1R/InsR function is required for axon extension and corpus callosum formation PLoS ONE, 2019;14(7):e0219362. 2019 [PMID: 31318893] (ICC, Mouse) ICC Mouse
Show All 148 Publications.

Reviews for beta-III Tubulin Antibody (MAB1195) (8) 4.68

Average Rating: 4.6
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Feline, Other.

Reviews using MAB1195:
Filter by Applications
IHC-Fr
(2)
WB
(1)
IF
(3)
ICC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 beta-III Tubulin MAB1195
Enlarge
reviewed by:
Jan Martinek
Mouse 01/17/2019
View

Summary

Sample TestedAdult mouse brain tissue
SpeciesMouse
LotHGQ0218071
Immunohistochemistry-Frozen beta-III Tubulin MAB1195
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 11/02/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult brain
SpeciesFeline
LotHGQ0217041
 
reviewed by:
Anonymous
WB Mouse 02/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23158765
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23158765

Details

Detection NotesSee PMID 23158765

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Mouse 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19409493
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19409493

Details

Detection NotesSee PMID 19409493

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22985741
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22985741

Details

Detection NotesSee PMID 22985741

Comments

CommentsIndependent review from antibodyresource.comExcellent antibody for use in IF and works at a dilution of 1:500 for adult human primary ligament cells.
 
reviewed by:
Anonymous
ICC Mouse 02/04/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 20722977
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20722977

Details

Detection NotesSee PMID 20722977

Comments

CommentsIndependent review from antibodyresource.comExcellent antibody for use in fixed, fresh frozen tissue or cultured neurons.
 
reviewed by:
Anonymous
IHC-Fr Other 02/04/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 22120963
SpeciesOther
Special ApplicationsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-III Tubulin Antibody (MAB1195) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin IC1195A
Biotin BAM1195
NL493 NL1195G
NL557 NL1195R
NL637 NL1195V
PerCP (Peridinin-chlorophyll Protein Complex) IC1195C

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Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Recent Reviews

4.6
5
5
4
3
3
0
2
0
1
0

Jan Martinek
01/17/2019
Application:
Species: Mouse

Anonymous
11/02/2017
Application: IHC-Fr
Species: Feline

 
Anonymous
02/04/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TUBB3