beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

Images

 
Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse ...read more
beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours ...read more
beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes ...read more
beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 ...read more
beta ‑III Tubulin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 8 ...read more
Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
TuJ-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific Summary

Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 8 Reviews rated 4.6
using
MAB1195 in the following applications:

Publications
Read Publications using
MAB1195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-III Tubulin Antibody (MAB1195)(126)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Amphibian, Avian - Quail, Axolotl, Axolotl - Ambystoma mexicanum, Chicken, Porcine.

We have publications tested in 17 applications: Flow, HSI Microscopy, ICC, IHC, IHC Frozen, IHC - Fresh, IHC - Paraffin embedded, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Fr, IHC-OCT embedded, IHC-P, IP, WB.


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Flow
(4)
HSI Microscopy
(1)
ICC
(66)
IHC
(15)
IHC Frozen
(1)
IHC - Fresh
(1)
IHC - Paraffin embedded
(2)
IHC Fresh
(5)
IHC Frozen
(8)
IHC Not Specified
(5)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(1)
IHC-Fr
(1)
IHC-OCT embedded
(1)
IHC-P
(1)
IP
(1)
WB
(10)
All Applications
Filter By Species
Human
(34)
Mouse
(61)
Rat
(20)
Amphibian
(1)
Avian - Quail
(1)
Axolotl
(2)
Axolotl - Ambystoma mexicanum
(1)
Chicken
(2)
Porcine
(2)
All Species
Showing Publications 1 - 10 of 126. Show All 126 Publications.
Publications using MAB1195 Applications Species
H Ogi, S Moriwaki, M Kokubo, Y Hikida, K Itoh Label-free classification of neurons and glia in neural stem cell cultures using a hyperspectral imaging microscopy combined with machine learning Sci Rep, 2019;9(1):633. 2019 [PMID: 30679652] (HSI Microscopy,, Human) HSI Microscopy Human
N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin Acta Neuropathol Commun, 2018;6(1):139. 2018 [PMID: 30541620] (ICC,, Mouse) ICC Mouse
Z Xu, P Fang, B Xu, Y Lu, J Xiong, F Gao, X Wang, J Fan, P Shi High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients Nat Commun, 2018;9(1):4745. 2018 [PMID: 30420609] (ICC,, Rat) ICC Rat
AM Lager, OG Corradin, JM Cregg, MS Elitt, HE Shick, BLL Clayton, KC Allan, HE Olsen, M Madhavan, PJ Tesar Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells Nat Commun, 2018;9(1):3708. 2018 [PMID: 30213958] (ICC,, Mouse) ICC Mouse
A Miko?ajczy, A Koz?owska, S Gonkowski Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures Int J Mol Sci, 2018;19(9):. 2018 [PMID: 30154361] (ICC,, Porcine) ICC Porcine
J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells Mol Autism, 2018;9(0):44. 2018 [PMID: 30147856] (IHC,, Human) IHC Human
SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 2018;15(1):226. 2018 [PMID: 30103769] (ICC,, Rat) ICC Rat
L Kisiswa, D Fernández-, MC Sergaki, CF Ibáñez RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival Cell Rep, 2018;24(4):1013-1024. 2018 [PMID: 30044969] (WB,, Mouse) WB Mouse
A Kannan, K Bhatia, D Branzei, L Gangwani Combined deficiency of Senataxin and DNA-PKcs causes DNA damage accumulation and neurodegeneration in spinal muscular atrophy Nucleic Acids Res., 2018;0(0):. 2018 [PMID: 30010942] (IHC-P,, Mouse) IHC-P Mouse
X Wen, AL Cahill, C Barta, WB Thoreson, S Nawy Elevated Pressure Increases Ca2+ Influx Through AMPA Receptors in Select Populations of Retinal Ganglion Cells Front Cell Neurosci, 2018;12(0):162. 2018 [PMID: 29950974] (ICC,, Mouse) ICC Mouse
Show All 126 Publications.

Reviews for beta-III Tubulin Antibody (MAB1195) (8) 4.68

Average Rating: 4.6
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Feline, Other.

Reviews using MAB1195:
Filter by Applications
IHC-Fr
(2)
WB
(1)
IF
(3)
ICC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 beta-III Tubulin MAB1195
Enlarge
reviewed by:
Jan Martinek
Mouse 01/17/2019
View

Summary

Sample TestedAdult mouse brain tissue
SpeciesMouse
LotHGQ0218071
Immunohistochemistry-Frozen beta-III Tubulin MAB1195
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 11/02/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult brain
SpeciesFeline
LotHGQ0217041
 
reviewed by:
Anonymous
WB Mouse 02/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23158765
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23158765

Details

Detection NotesSee PMID 23158765

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Mouse 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19409493
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19409493

Details

Detection NotesSee PMID 19409493

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22985741
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22985741

Details

Detection NotesSee PMID 22985741

Comments

CommentsIndependent review from antibodyresource.comExcellent antibody for use in IF and works at a dilution of 1:500 for adult human primary ligament cells.
 
reviewed by:
Anonymous
ICC Mouse 02/04/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 20722977
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20722977

Details

Detection NotesSee PMID 20722977

Comments

CommentsIndependent review from antibodyresource.comExcellent antibody for use in fixed, fresh frozen tissue or cultured neurons.
 
reviewed by:
Anonymous
IHC-Fr Other 02/04/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 22120963
SpeciesOther
Special ApplicationsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-III Tubulin Antibody (MAB1195) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled BAM1195

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Bioinformatics Tool for beta-III Tubulin Antibody (MAB1195)

Discover related pathways, diseases and genes to beta-III Tubulin Antibody (MAB1195). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for beta-III Tubulin Antibody (MAB1195)

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Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Recent Reviews

4.6
5
5
4
3
3
0
2
0
1
0

Jan Martinek
01/17/2019
Application:
Species: Mouse

Anonymous
11/02/2017
Application: IHC-Fr
Species: Feline

 
Anonymous
02/04/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TUBB3