AIF-1/Iba1 Antibody

Images

 
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Mouse (A) and Rat (B) Brain lysate with antibody at 0.5 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - A20 silencing prevents the anti-inflammatory effects of EA after focal cerebral ischemia reperfusion. Experiments were performed 24 h after ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). Antibody at 1:250. ...read more
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC image submitted by a verified customer review.
Immunohistochemistry Whole-Mount: AIF-1/Iba1 Antibody [NB100-1028] - Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining in rat hippocampus using AIF-1/Iba1 Antibody. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Human glioma. PDIA3 staining in human GB specimens: the photograph shows a representative tumor field for PDIA3 and IBA1 (magnification: 40x). Double ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Canine liver stained for Iba1 (brown) and counterstained with Richard-Allan Scientific hematoxylin. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - AIF-1/Iba1 in canine brain. Primary antibody used at 1 ug/mL and incubated with tissue for 30 minutes at room temperature. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining of paraffin embedded Human Spleen with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Predicted Species
Porcine (100%), Bovine (92%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 4 - 6 ug/mL
  • Knockout Validated
  • Peptide ELISA Detection Limit 1:64000
  • Western Blot 0.3 - 1.0 ug/mL
Application Notes
ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). This AIF-1/Iba1 antibody is validated for IHC-Whole mount and IHC-F from verified customer reviews. Dual ISH-IHC validation reported in scientific literature (PMID: 31220182).
Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AIF-1/Iba1 Overexpression Lysate
Reviewed Applications
Read 21 Reviews rated 4.4
using
NB100-1028 in the following applications:

Publications
Read Publications using
NB100-1028 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID: 33704579). Feline and canine reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NB100-1028)(207)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: Dual ISH-IHC, ICC/IF, IHC, IHC-F, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, KO, WB.


Filter By Application
Dual ISH-IHC
(1)
ICC/IF
(48)
IHC
(57)
IHC-F
(9)
IHC-FR
(1)
IHC-Fr
(17)
IHC-FrFl
(6)
IHC-P
(5)
IHC-WhMt
(1)
KO
(1)
WB
(13)
All Applications
Filter By Species
Human
(8)
Mouse
(108)
Rat
(34)
All Species
Showing Publications 1 - 10 of 207. Show All 207 Publications.
Publications using NB100-1028 Applications Species
Yoon S, Hong N, Lee M, Ahn J Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture Cells Jul 2 2021 [PMID: 34359834] (ICC/IF) ICC/IF
Mayagoitia K, Tolan Aj, Shammi S Et Al. Loss of APP in mice increases thigmotaxis and is associated with elevated brain expression of IL-13 and IP-10/CXCL10 Physiology & behavior Jul 19 2021 [PMID: 34293404] (IHC) IHC
Saddala Ms, Yang X, Tang S, Huang H Transcriptome-wide analysis reveals core sets of transcriptional regulators of sensome and inflammation genes in retinal microglia Genomics Jul 7 2021 [PMID: 34242709] (ICC/IF) ICC/IF
Du H, Wong MY, Zhang T et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses Life science alliance Jul 1 2021 [PMID: 34103390] (Mouse) Mouse
Leduc-Pessah H, Xu C, Fan CY et al. Spinal A3 adenosine receptor activation acutely restores morphine antinociception in opioid tolerant male rats Journal of neuroscience research Jun 1 2021 [PMID: 34075613]
Zhou L, Tao X, Pang G et al. Maternal Nicotine Exposure Alters Hippocampal Microglia Polarization and Promotes Anti-inflammatory Signaling in Juvenile Offspring in Mice Frontiers in pharmacology May 11 2021 [PMID: 34045967] (IHC, Mouse) IHC Mouse
Lin X, Zhan J, Jiang J, Ren Y Upregulation of Neuronal Cylindromatosis Expression is Essential for Electroacupuncture-Mediated Alleviation of Neuroinflammatory Injury by Regulating Microglial Polarization in Rats Subjected to Focal Cerebral Ischemia/Reperfusion Journal of inflammation research May 20 2021 [PMID: 34045881] (IHC, Rat) IHC Rat
Isaac AR, de Velasco PC, Fraga KYD et al. Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny`s neocortex and hippocampus: impact on synaptic markers: n-3 maternal diet sway progeny`s synaptic markers The Journal of nutritional biochemistry May 23 2021 [PMID: 34038760]
Derkach D, Kehtari T, Renaud M et al. Metformin pretreatment rescues olfactory memory associated with subependymal zone neurogenesis in a juvenile model of cranial irradiation Cell Reports Medicine Apr 1 2021 [PMID: 33948569] (IHC, Mouse) IHC Mouse
Vegh C, Wear D, Okaj I et al. Combined Ubisol-Q10 and Ashwagandha Root Extract Target Multiple Biochemical Mechanisms and Reduces Neurodegeneration in a Paraquat-Induced Rat Model of Parkinsons Disease Antioxidants Apr 6 2021 [PMID: 33917328] (IHC, Rat) IHC Rat
Show All 207 Publications.

Reviews for AIF-1/Iba1 Antibody (NB100-1028) (21) 4.421

Average Rating: 4.4
(Based on 21 reviews)
We have 21 reviews tested in 6 species: Human, Mouse, Rat, Canine, Feline, Human and Mouse.

Reviews using NB100-1028:
Filter by Applications
IHC-P
(6)
ICC
(2)
IHC-Fr
(5)
IF
(5)
All Applications
Filter by Species
Human
(1)
Mouse
(12)
Rat
(2)
Canine
(3)
Feline
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IHC-P Canine 10/29/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain
SpeciesCanine
LotS7C7G2P39
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IHC-P Canine 07/03/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver
SpeciesCanine
LotS7C7G2P41

Comments

CommentsFollowing deparaffinization, pre-heated >=90C Target Retrieval Solution - Dako S1699 - was added to slides and then the slide container was placed into a vegetable steamer for 20min. Following antigen retrieval, slides were cooled for 15min, washed with 1X PBS, then placed in 3 percent hydrogen peroxide diluted in methanol. Following peroxide quench, slides were sequentially incubated - 10min each at room temperature with washes in between - in avidin and biotin solution -abcam ab64212- then Dako Serum-Free Protein Block - Dako X0909. After incubation with the protein block, slides were incubated with the primary antibody diluted 1:500 in Dako Antibody Diluent - Dako S3022 - for 30 min at room temperature. Slides were then incubated with rabbit anti-goat - Vector Labs BA5000 - diluted at 1:200 in Dako Serum-Free Protein Block - Dako X0909. Slides were then incubated with VECTASTAIN Elite ABC-HRP Reagent - Vector Labs PK-7100 - for 30min at room temperature. Staining was then developed using Dako DAB as a chromogen.
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 08/12/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult mouse brain tissue
SpeciesMouse
LotS7c7G2p41

Comments

Comments1/100 dilution Overnight in PBS at 4*C
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
Lucia Lisi
IHC-P Human 01/17/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain tissue,Brain Tissue Sections,Human Brain Tissue
SpeciesHuman
LotS9C3G2
 AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Emily Thompson
Mouse 12/14/2019
View

Summary

Sample TestedPFA fixed mouse brain,SCID mouse cortex injected with human glioblastoma cells
SpeciesMouse
LotS7C7G2P13

Comments

Comments1:250
Immunocytochemistry AIF-1/Iba1 NB100-1028
Enlarge
3
reviewed by:
Verified Customer
ICC Mouse 11/21/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedadult mouse neural stem cells
SpeciesMouse
Lot87C7G2P26
 AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
J. Sebastián Jara
Mouse 08/12/2019
View

Summary

Sample TestedDorsal root ganglion
SpeciesMouse
LotS7C7G2P34E161018

Comments

CommentsGood signal to noise ratio for 3D imaging.
***Novus Innovators Program - new species or application used on a primary antibody.
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Feline 07/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedoptic nerve
SpeciesFeline
LotS9G2P3E240918
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
1
reviewed by:
Verified Customer
IHC-Fr Mouse 05/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotSC7G2P23
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotS7G7G2P19
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
Verified Customer
IHC-P Canine 08/07/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOptic nerve
SpeciesCanine
LotS7C7G2P15
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IF Mouse 04/23/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested Mouse brain
SpeciesMouse
LotS7G7G2P19
 AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Fuyi Chen
Human and Mouse 03/16/2018
View

Summary

Sample Tested Mouse brain
SpeciesHuman and Mouse
LotS7G7G2P15E031117
  5
reviewed by:
Haiyan Qiu
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
3
reviewed by:
Verified Customer
IHC-Fr Mouse 09/22/2016
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
Verified Customer
IF Mouse 05/11/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
LotS7C7P6

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, RT

Primary Anitbody

Dilution Ratio1/500, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555
Secondary Manufacturer Cat#A21432
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
Verified Customer
IHC-Fr Rat 10/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat frozen 4% paraformalehyde fixed brain sections
SpeciesRat
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Xin Luo
IHC-P Mouse 06/30/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsTNB, 15 min, room temp

Primary Anitbody

Dilution Ratio1:20 in TNB, 60 min, room temp

Secondary Antibody

Secondary DescriptionDonkey anti-goat IgG, Cy5.5
Secondary Manufacturer Cat#NB120-6951
Secondary Concentration1:20

Details

Detection NotesEVOS microscope with APC cube
Fixation DetailsAntigen retrieval with citrate buffer, 98C for 30 min, cool 20 min
Wash DescriptionPBS, 2x of 4 min each
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Jaycie Loewen
IF Mouse 07/22/2013
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Grace zhou
IF Rat 07/16/2012
View

Summary

ApplicationImmunofluorescence
Sample Tested brain and spinal cord
SpeciesRat
Lots5c2g2

Blocking

Blocking DetailsPBS-T

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionANTI-GOAT
Secondary Manufacturer Cat#Jackson immunoresearch
Secondary Concentration1:1000

Details

Fixation Details4%PFA
Wash DescriptionPBS-T
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IF 06/26/2012
View

Summary

ApplicationImmunofluorescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).

  1. What is the volume of the sample size (NB100-1028SS)?
    • According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml.  Therefore, the vial should contain 40 ul.
  2. The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?
    • With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol:https://www.novusbio.com/support/support-by-application.html

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.4
5
13
4
5
3
2
2
0
1
1

Verified Customer
10/29/2021
Application: IHC-P
Species: Canine

Verified Customer
07/03/2021
Application: IHC-P
Species: Canine

Verified Customer
08/12/2020
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot