AIF-1/Iba1 Antibody

Images

 
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Human Lymph node (Molecular weight: 16.7 KDa) (A), Mouse Brain (B) and Rat Brain (C) lysate with AIF-1/Iba1 Antibody at 1 ug/mL (35 ug protein in RIPA ...read more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - A20 silencing prevents the anti-inflammatory effects of EA after focal cerebral ischemia reperfusion. Experiments were performed 24 h after ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining of paraffin embedded Mouse Lung using AIF-1/Iba1 Antibody. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining (1:500).
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). at 1:250. IHC image ...read more
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using. IHC image submitted by a verified customer review.
Immunohistochemistry Whole-Mount: AIF-1/Iba1 Antibody [NB100-1028] - Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining in rat hippocampus using AIF-1/Iba1 Antibody at 40X. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Predicted Species
Porcine (100%), Bovine (92%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection Limit 1:128000
  • Dual RNAscope ISH-IHC
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Knockout Validated
Application Notes
ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). AIF-1/Iba1 antibody validated for IHC-Whole mount, IHC-F from verified customer reviews. Dual ISH-IHC validation (PMID: 31220182).
Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AIF-1/Iba1 Overexpression Lysate
Reviewed Applications
Read 17 Reviews rated 4.3
using
NB100-1028 in the following applications:

Publications
Read Publications using
NB100-1028 in the following applications:

Reactivity Notes

Expected from sequence similarity: Porcine. Mouse reactivity reported in scientific literature (PMID: 26490872). Canine, Feline reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NB100-1028)(135)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: Dual ISH-IHC, ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, WB.


Filter By Application
Dual ISH-IHC
(1)
ICC/IF
(28)
IHC
(35)
IHC-FR
(1)
IHC-Fr
(15)
IHC-FrFl
(6)
IHC-P
(11)
IHC-WhMt
(1)
WB
(12)
All Applications
Filter By Species
Human
(5)
Mouse
(75)
Rat
(23)
All Species
Showing Publications 1 - 10 of 135. Show All 135 Publications.
Publications using NB100-1028 Applications Species
Burkovetskaya ME, Small R, Guo L et al. Cocaine self-administration differentially activates microglia in the mouse brain Neurosci. Lett. Apr 9 2020 [PMID: 32278944] (WB, Mouse) WB Mouse
Muke I, Sprenger A, Bobylev I et Al. Ultrastructural characterization of mitochondrial damage in experimental autoimmune neuritis J. Neuroimmunol. Mar 20 2020 [PMID: 32251941] (IHC, Rat) IHC Rat
Lisi L, Pia Ciotti G, Chiavari M et Al. Vascular endothelial growth factor receptor 1 in glioblastoma associated microglia/macrophages Oncol Rep Mar 19 2020 [PMID: 32236632]
Tonello R, Xie W, Lee SH et al. Local Sympathectomy Promotes Antiinflammatory Responses and Relief of Paclitaxel-induced Mechanical and Cold Allodynia in Mice Anesthesiology Mar 30 2020 [PMID: 32235156] (IHC, Mouse) IHC Mouse
Gattlen C, Deftu AF, Tonello R et al. The inhibition of Kir2.1 potassium channels depolarizes spinal microglial cells, reduces their proliferation, and attenuates neuropathic pain Glia Mar 27 2020 [PMID: 32220118] (IHC, IHC-FrFl, Mouse) IHC, IHC-FrFl Mouse
Zhu X, Hatfield J, Sullivan Jk et Al. Robust neuroinflammation and perivascular pathology in rTg-DI rats, a novel model of microvascular cerebral amyloid angiopathy J Neuroinflammation Mar 4 2020 [PMID: 32127016] (IHC, Rat) IHC Rat
Neves J, Chirco Kr, Cedron-Craft W et Al. MANF delivery improves retinal homeostasis and cell replacement therapies in ageing mice Exp. Gerontol. Feb 28 2020 [PMID: 32119994] (IHC, Mouse) IHC Mouse
Zhou X, Wahane S, Friedl Ms et Al. Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci. Mar 1 2020 [PMID: 32112058] (IHC-P, Mouse) IHC-P Mouse
Nardai S, LAszlO M, SzabO A et al. N,N-dimethyltryptamine reduces infarct size and improves functional recovery following transient focal brain ischemia in rats Exp. Neurol. Feb 14 2020 [PMID: 32067950] (WB, Rat) WB Rat
Cap K, Jung Y, Choi B et al. Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different A beta concentrations Redox Biology Jan 1 2020 [PMID: 32046944] (ICC/IF, Mouse) ICC/IF Mouse
Show All 135 Publications.

Reviews for AIF-1/Iba1 Antibody (NB100-1028) (17) 4.317

Average Rating: 4.3
(Based on 17 reviews)
We have 17 reviews tested in 6 species: Human, Mouse, Rat, Canine, Feline, Mouse and Human.

Reviews using NB100-1028:
Filter by Applications
IHC-P
(3)
ICC
(2)
IHC-Fr
(5)
IF
(5)
All Applications
Filter by Species
Human
(1)
Mouse
(10)
Rat
(2)
Canine
(1)
Feline
(1)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
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reviewed by:
Lucia Lisi
IHC-P Human 01/17/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain Tissue Sections,Human Brain Tissue,Brain tissue
SpeciesHuman
LotS9C3G2
 AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Emily Thompson
Mouse 12/14/2019
View

Summary

Sample TestedPFA fixed mouse brain,SCID mouse cortex injected with human glioblastoma cells
SpeciesMouse
LotS7C7G2P13

Comments

Comments1:250
Immunocytochemistry AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
ICC Mouse 11/21/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedadult mouse neural stem cells
SpeciesMouse
Lot87C7G2P26
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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reviewed by:
Anonymous
IHC-Fr Feline 07/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedoptic nerve
SpeciesFeline
LotS9G2P3E240918
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 05/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotSC7G2P23
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotS7G7G2P19
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-P Canine 08/07/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOptic nerve
SpeciesCanine
LotS7C7G2P15
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF Mouse 04/23/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested Mouse brain
SpeciesMouse
LotS7G7G2P19
 AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Fuyi Chen
Mouse and Human 03/16/2018
View

Summary

Sample Tested Mouse brain
SpeciesMouse and Human
LotS7G7G2P15E031117
 
reviewed by:
Haiyan Qiu
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 09/22/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF Mouse 05/11/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
LotS7C7P6

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, RT

Primary Anitbody

Dilution Ratio1/500, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555
Secondary Manufacturer Cat#A21432
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Rat 10/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat frozen 4% paraformalehyde fixed brain sections
SpeciesRat
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
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reviewed by:
Xin Luo
IHC-P Mouse 06/30/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsTNB, 15 min, room temp

Primary Anitbody

Dilution Ratio1:20 in TNB, 60 min, room temp

Secondary Antibody

Secondary DescriptionDonkey anti-goat IgG, Cy5.5
Secondary Manufacturer Cat#NB120-6951
Secondary Concentration1:20

Details

Detection NotesEVOS microscope with APC cube
Fixation DetailsAntigen retrieval with citrate buffer, 98C for 30 min, cool 20 min
Wash DescriptionPBS, 2x of 4 min each
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Jaycie Loewen
IF Mouse 07/22/2013
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Grace zhou
IF Rat 07/16/2012
View

Summary

ApplicationImmunofluorescence
Sample Tested brain and spinal cord
SpeciesRat
Lots5c2g2

Blocking

Blocking DetailsPBS-T

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionANTI-GOAT
Secondary Manufacturer Cat#Jackson immunoresearch
Secondary Concentration1:1000

Details

Fixation Details4%PFA
Wash DescriptionPBS-T
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF 06/26/2012
View

Summary

ApplicationImmunofluorescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).

  1. What is the volume of the sample size (NB100-1028SS)?
    • According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml.  Therefore, the vial should contain 40 ul.
  2. The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?
    • With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol:https://www.novusbio.com/support/support-by-application.html

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AIF-1/Iba1 Antibody (NB100-1028)

Discover related pathways, diseases and genes to AIF-1/Iba1 Antibody (NB100-1028). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for AIF-1/Iba1 Antibody (NB100-1028)

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Recent Reviews

4.3
5
10
4
4
3
2
2
0
1
1

Lucia Lisi
01/17/2020
Application: IHC-P
Species: Human

Emily Thompson
12/14/2019
Application:
Species: Mouse

Anonymous
11/21/2019
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot