AIF-1/Iba1 Antibody

Images

 
GM1 effects on brain microglia in vivo, after peripheral administration of LPS. Immunoblot in whole brain lysates and densitometric analysis show LPS-dependent increase in AIF-1/Iba1 expression. Image collected and ...read more
Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). Antibody at 1:250. IHC image submitted by a verified customer review.
AIF-1/Iba1 in canine brain. Primary antibody used at 1 ug/mL and incubated with tissue for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.
Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.
Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a verified customer review.
Staining in rat hippocampus using AIF-1/Iba1 Antibody. IHC-Fr image submitted by a verified customer review.
Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. IHC-Fr image submitted by a verified customer review.
Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a verified customer review.
Staining in mouse brain using AIF-1/Iba1 Antibody. IHC image submitted by a verified customer review.
Analysis of LPS injected mouse brain tissue, 20 um thickness, frozen. Primary antibody dilution, 1:200. Secondary antibody CY3. Mounted with DAPI mount. Image from verified customer review.
AIF-1/Iba1 staining of AD mouse model (hippocampus). Image from verified customer review.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
(0.1ug/ml) staining of Mouse Lymph node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
(1ug/ml) staining of Human Frontal Cortex (A) and (0.1ug/ml) Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
(8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
GM1 effects on brain microglia in vivo, after peripheral administration of LPS. C) Immunoblot in whole brain lysates and densitometric analysis show LPS-dependent increase in Iba 1 expression. Image collected and ...read more
CXCR2+neutrophils are not required for microglial activation. a Representative images of Iba1 immunohistochemistry staining in lumbar spinal cord ventral horns of naive control, control EAE, and Cxcr2 cKO EAE mice. ...read more
Deficiency of HDAC3 in microglia suppresses the cGAS-STING-mediated autoimmune response. (K) Proteins extracted from the ischemic penumbra and contralateral cortex of HDAC3 cKO and WT mice subjected to tMCAO 6 h ...read more
ADT induced SPINK1 upregulation associates with NE-phenotype in mice and NEPC patients.a Box plots depicting relative expression of SPINK1, SYP, CHGA, TUBB3, and VIM transcripts (read counts) in VCaP tumors implanted ...read more
EBNA1 promotes the upregulation of oxidative DNA damage repair pathways in EBV converted cell lines and EBV positive BLs. a Representative western blots illustrating the expression of MTH1, OGG1, and MUTYH in pairs of ...read more
Rab31 expression levels in stably transfected breast cancer cells. (A) pAb RT3-IgG specifically detects rab31 in cell lysates from stably transfected MDA-MB-231 and CAMA-1 breast cancer cells in Western blot analyses. ...read more
EGFR inhibition downregulates VEGF and HIF-1 in vitro and in vivo.SQ20B cells were seeded at 25% confluence and cultured overnight. The next morning, cells were transfected with 150 nanomoles siRNA (either EGFR ...read more
Elevated levels of additional proteins and GM3 ganglioside in the MEC of MPS IIIB brain.Staining performed with antibodies to the indicated substances was observed in the MEC region of 3 month-old MPS IIIB mice (for ...read more
ERK2 utilizes kinase-docking domains to interact with ATG5–ATG12 and LC3-II.(a) Mutations in FRS on ERK2 decrease colocalization of ERK2 with ATG5–ATG12, LC3 and WIPI1. Immunofluorescence (IF) showing colocalization ...read more
Human tumor microarrays (TMAs).Pilocytic astrocytoma TSPO immunohistochemistry (A) and TSPO immunohistochemical scoring (C). Glioblastoma multiforme TSPO immunohistochemistry (B) and TSPO immunohistochemical scoring ...read more
DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes.(A) Whole mount immunofluorescence co-staining with anti-DNMT1 and DAPI on the same Nlrp2M+/Z+ embryos used to ...read more
Activation of MAPK7 in chondrocytes is essential for hypoxic adaptation and enhancement of HIF1 alpha signaling under hypoxia. a, b Immunofluorescence staining of (a) MAPK7 (b) P-MAPK7 on representative sections of ...read more
TRF2 transcriptionally regulates p21 expression through promoter occupancy. (A) Quantitative ChIP using TRF2 antibody gives enriched binding of TRF2 on p21 promoter in HT1080 and MDA-MB-231 cells, IgG was used as ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Predicted Species
Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 6 - 8 ug/mL
  • Knockout Validated
  • Peptide ELISA Detection Limit 1:128000
  • Western Blot 0.1 - 1.0 ug/mL
Application Notes
ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). This AIF-1/Iba1 antibody is validated for IHC-Whole mount and IHC-F from verified customer reviews. Dual ISH-IHC validation reported in scientific literature (PMID: 31220182). WB: Approx. 16kDa band observed in Human Frontal Cortex, Mouse Brain and Rat Brain lysates and Mouse Lymph node lysates (calculated MW of 16.7kDa according to Human NP_001614.3, 16.9kDa according to Mouse NP_062340.1 and 16.8kDa according to Rat NP_058892.1). Primary incubation 1 hour at room temperature.
Theoretical MW
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AIF-1/Iba1 Overexpression Lysate
Reviewed Applications
Read 23 Reviews rated 4.4
using
NB100-1028 in the following applications:

Publications
Read Publications using
NB100-1028 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID: 33704579). Feline and canine reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIF-1/Iba1 Antibody (NB100-1028)(384)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Horse, Porcine.

We have publications tested in 15 applications: Dual ISH-IHC, ELISA, ICC, ICC/IF, IF/IHC, IHC, IHC-F, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, IP, In Vivo, KO, WB.


Filter By Application
Dual ISH-IHC
(1)
ELISA
(2)
ICC
(1)
ICC/IF
(69)
IF/IHC
(76)
IHC
(26)
IHC-F
(9)
IHC-Fr
(48)
IHC-FrFl
(13)
IHC-P
(21)
IHC-WhMt
(3)
IP
(1)
In Vivo
(2)
KO
(1)
WB
(24)
All Applications
Filter By Species
Human
(16)
Mouse
(181)
Rat
(45)
Feline
(1)
Horse
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 384. Show All 384 Publications.
Publications using NB100-1028 Applications Species
Wu DM, Liu JP, Liu J et al. Immune pathway activation in neurons triggers neural damage after stroke Cell reports 2023-11-01 [PMID: 37917581] (IHC, Mouse) IHC Mouse
Greenman R, Segal-Salto M, Barashi N et al. CCL24 regulates biliary inflammation and fibrosis in primary sclerosing cholangitis JCI insight 2023-06-22 [PMID: 37345655] (IHC-P, Mouse) IHC-P Mouse
Zheng G, Harms AK, Tail M et al. Effects of a neurokinin-1 receptor antagonist in the acute phase after thoracic spinal cord injury in a rat model Frontiers in molecular neuroscience 2023-05-12 [PMID: 37251648] (IHC-P, Mouse)

Details:
1:200 IHC-P dilution
IHC-P Mouse
Choconta JL, Labi V, Dumbraveanu C et al. Age-related neuroimmune signatures in dorsal root ganglia of a Fabry disease mouse model Immunity & ageing : I & A 2023-05-12 [PMID: 37173694] (IHC, Mouse)

Details:
IHC 1:100, Antigen rerieval: sodium citrate 80C/30min
IHC Mouse
Medina-Rodriguez EM, Han D, Lowell J, Beurel E Stress promotes the infiltration of peripheral immune cells to the brain Brain, behavior, and immunity 2023-05-09 [PMID: 37169132]
Qi, L;Li, X;Zhang, F;Zhu, X;Zhao, Q;Yang, D;Hao, S;Li, T;Li, X;Tian, T;Feng, J;Sun, X;Wang, X;Gao, S;Wang, H;Ye, J;Cao, S;He, Y;Wang, H;Wei, B; VEGFR-3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection Cell reports 2023-05-10 [PMID: 37167063]
Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ; Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury Cell reports 2023-05-06 [PMID: 37149868] (ICC/IF) ICC/IF
Zhang, T;Pang, W;Feng, T;Guo, J;Wu, K;Nunez Santos, M;Arthanarisami, A;Nana, AL;Nguyen, Q;Kim, PJ;Jankowsky, JL;Seeley, WW;Hu, F; TMEM106B regulates microglial proliferation and survival in response to demyelination Science advances 2023-05-05 [PMID: 37146150] (WB, Mouse) WB Mouse
Zhou J, Lin S, Hu Q et al. Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury Investigative ophthalmology & visual science 2023-05-01 [PMID: 37145604] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 384 Publications.

Reviews for AIF-1/Iba1 Antibody (NB100-1028) (23) 4.423

Average Rating: 4.4
(Based on 23 reviews)
We have 23 reviews tested in 6 species: Human, Mouse, Rat, Canine, Feline, Human and Mouse.

Reviews using NB100-1028:
Filter by Applications
IHC-Fr
(7)
IHC-P
(6)
Immunohistochemistry-PFA fixed, free floating
(1)
ICC
(2)
Whole-mount tissue immunolabeling
(1)
IF
(5)
immunohistochemistry free floating tissue
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(14)
Rat
(2)
Canine
(3)
Feline
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/27/2023
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain
SpeciesMouse
LotS7C7G2P44
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Deepak Gupta
IHC-Fr Mouse 04/25/2023
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested20 um Mouse brain Tissue
SpeciesMouse
Lot87C7G2P41
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
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5
reviewed by:
Verified Customer
IHC-P Canine 10/29/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain
SpeciesCanine
LotS7C7G2P39
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IHC-P Canine 07/03/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver
SpeciesCanine
LotS7C7G2P41

Comments

CommentsFollowing deparaffinization, pre-heated >=90C Target Retrieval Solution - Dako S1699 - was added to slides and then the slide container was placed into a vegetable steamer for 20min. Following antigen retrieval, slides were cooled for 15min, washed with 1X PBS, then placed in 3 percent hydrogen peroxide diluted in methanol. Following peroxide quench, slides were sequentially incubated - 10min each at room temperature with washes in between - in avidin and biotin solution -abcam ab64212- then Dako Serum-Free Protein Block - Dako X0909. After incubation with the protein block, slides were incubated with the primary antibody diluted 1:500 in Dako Antibody Diluent - Dako S3022 - for 30 min at room temperature. Slides were then incubated with rabbit anti-goat - Vector Labs BA5000 - diluted at 1:200 in Dako Serum-Free Protein Block - Dako X0909. Slides were then incubated with VECTASTAIN Elite ABC-HRP Reagent - Vector Labs PK-7100 - for 30min at room temperature. Staining was then developed using Dako DAB as a chromogen.
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
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5
reviewed by:
Verified Customer
IHC-P Mouse 08/12/2020
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult mouse brain tissue
SpeciesMouse
LotS7c7G2p41

Comments

Comments1/100 dilution Overnight in PBS at 4*C
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
Lucia Lisi
IHC-P Human 01/17/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain Tissue Sections,Brain tissue,Human Brain Tissue
SpeciesHuman
LotS9C3G2
Other AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Emily Thompson
Immunohistochemistry-PFA fixed, free floating Mouse 12/14/2019
View

Summary

ApplicationOther
Sample TestedPFA fixed mouse brain,SCID mouse cortex injected with human glioblastoma cells
SpeciesMouse
LotS7C7G2P13

Comments

Comments1:250
Immunocytochemistry AIF-1/Iba1 NB100-1028
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3
reviewed by:
Verified Customer
ICC Mouse 11/21/2019
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Summary

ApplicationImmunocytochemistry
Sample Testedadult mouse neural stem cells
SpeciesMouse
Lot87C7G2P26
Other AIF-1/Iba1 NB100-1028
Enlarge
4
reviewed by:
J. Sebastián Jara
Whole-mount tissue immunolabeling Mouse 08/12/2019
View

Summary

ApplicationOther
Sample TestedDorsal root ganglion
SpeciesMouse
LotS7C7G2P34E161018

Comments

CommentsGood signal to noise ratio for 3D imaging.
***Novus Innovators Program - new species or application used on a primary antibody.
  5
reviewed by:
Verified Customer
IHC-Fr Feline 07/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedoptic nerve
SpeciesFeline
LotS9G2P3E240918
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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1
reviewed by:
Verified Customer
IHC-Fr Mouse 05/15/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotSC7G2P23
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotS7G7G2P19
  4
reviewed by:
Verified Customer
IHC-P Canine 08/07/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOptic nerve
SpeciesCanine
LotS7C7G2P15
IF AIF-1/Iba1 NB100-1028
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5
reviewed by:
Verified Customer
IF Mouse 04/23/2018
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Summary

ApplicationIF
Sample TestedMouse brain
SpeciesMouse
LotS7G7G2P19
Other AIF-1/Iba1 NB100-1028
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5
reviewed by:
Fuyi Chen
immunohistochemistry free floating tissue Human and Mouse 03/16/2018
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Summary

ApplicationOther
Sample TestedMouse brain
SpeciesHuman and Mouse
LotS7G7G2P15E031117
  5
reviewed by:
Haiyan Qiu
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedbrain and spinal cord
SpeciesMouse
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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3
reviewed by:
Verified Customer
IHC-Fr Mouse 09/22/2016
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
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4
reviewed by:
Verified Customer
IF Mouse 05/11/2016
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Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
LotS7C7P6
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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4
reviewed by:
Verified Customer
IHC-Fr Rat 10/08/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat frozen 4% paraformalehyde fixed brain sections
SpeciesRat
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Xin Luo
IHC-P Mouse 06/30/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
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5
reviewed by:
Jaycie Loewen
IF Mouse 07/22/2013
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Summary

ApplicationImmunofluorescence
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Grace zhou
IF Rat 07/16/2012
View

Summary

ApplicationImmunofluorescence
Sample Testedbrain and spinal cord
SpeciesRat
Lots5c2g2
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
5
reviewed by:
Verified Customer
IF 06/26/2012
View

Summary

ApplicationImmunofluorescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).

  1. What is the volume of the sample size (NB100-1028SS)?
    • According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml.  Therefore, the vial should contain 40 ul.
  2. The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?
    • With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.4
5
15
4
5
3
2
2
0
1
1

Verified Customer
04/27/2023
Application: IHC-Fr
Species: Mouse

Deepak Gupta
04/25/2023
Application: IHC-Fr
Species: Mouse

Verified Customer
10/29/2021
Application: IHC-P
Species: Canine

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot