AIF-1/Iba1 Antibody

Images

 
Immunocytochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Analysis of Iba1 in rat brain and spinal cord. Image courtesy of anonymous customer product review.
Immunocytochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Analysis of Iba1 in rat samples. Image courtesy of product review submitted by Tatyana Kanyshkova.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Iba-1 in mouse brain. Image from verified customer review.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining of frozen rat hippocampal tissue with AIF-1 antibody. Image from verified customer review.
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - (1ug/ml) staining of Human Frontal Cortex (A) Rat (B) Mouse (C) and Pig (D) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - Mouse hypothalamus. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
Peptide with sequence C-TGPPAKKAISELP corresponding to C-Terminus according to NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF1 / IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection Limit 1:128000
  • Immunocytochemistry 1:10-1:500
Application Notes
Western blot: Approx 18 kDa band observed in human brain (frontal cortex), 17 kDa in mouse brain and 16 kDa in rat brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3, and 16.8 kDa according to Mouse NP_062340.1 and Rat NP_058892.1). Primary incubation 1 hour at room temperature.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
AIF-1/Iba1 Lysate (NBL1-07408)
Reviewed Applications
Read 10 Reviews rated 4.6
using
NB100-1028 in the following applications:

Publications
Read Publications using
NB100-1028 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26490872).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • AIF-1IRT1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IBA1Em:AF129756.17
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NB100-1028)(56)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(9)
IHC
(7)
IHC-FR
(1)
IHC-Fr
(7)
IHC-FrFl
(3)
IHC-P
(3)
IHC-WhMt
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(21)
Rat
(11)
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using NB100-1028 Applications Species
Xu H, Qin W, Hu X et al. Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-kB signaling pathway in cerebral ischemia/reperfusion rats. J Neuroinflammation. Mar 15 2018 [PMID: 29544517] (ICC/IF, Rat) ICC/IF Rat
Zhang R, Wu Y, Xie F, Zhong Y. RGMa mediates reactive astrogliosis and glial scar formation through TGFb1/Smad2/3 signaling after stroke. Cell Death Differ. 2018 Feb 02 [PMID: 29396549] (ICC/IF, Rat) ICC/IF Rat
Cooper SG, Trivedi DP, Yamamoto R et al. Increased (Pro) renin Receptor Expression in Subfornical Organ Neurons in Hypertensive Humans. American Journal of Physiology-Heart and Circulatory Physiology 2017 Dec 22 [PMID: 29351470] (ICC/IF, Human) ICC/IF Human
Chang LH, Lin HC, Huang SS et al. Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation Sci Rep 2018 Jan 08 [PMID: 29311641] (IHC-Fr, Mouse) IHC-Fr Mouse
Marques-Torrejon MA, Gangoso E, Pollard SM. Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture. Dis Model Mech 2017 Dec 01 [PMID: 29196443] (Mouse) Mouse
Choi JY, Jang JS, Son DJ et al. Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment. Int J Mol Sci 2017 Nov 28 [PMID: 29182579] (Mouse) Mouse
Jiang J, Luo Y, Qin W et al. Electroacupuncture Suppresses the NF-kB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats Front Mol Neurosci. 2017 Nov 06 [PMID: 29163038] (ICC/IF, Rat) ICC/IF Rat
Kuboyama T, Wahane S, Huang Y et al. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation Sci Rep 2017 Aug 17 [PMID: 28819194] (IHC, Mouse) IHC Mouse
Langseth AJ, Kim J, Ugolino JE et al. Cell-type specific differences in promoter activity of the ALS-linked C9orf72 mouse ortholog Sci Rep 2017 Jul 18 [PMID: 28720882] (IHC-FR, Mouse) IHC-FR Mouse
Choi JY, Hwang CJ, Lee HP et al. Inhibitory effect of ethanol extract of Nannochloropsis oceanica on lipopolysaccharide-induced neuroinflammation, oxidative stress, amyloidogenesis and memory impairment Oncotarget 2017 Jul 11 [PMID: 28489589] (IHC-P, Mouse) IHC-P Mouse
Show All 56 Publications.

Reviews for AIF-1/Iba1 Antibody (NB100-1028) (10) 4.610

Average Rating: 4.6
(Based on 10 reviews)
We have 10 reviews tested in 3 species: Mouse, Rat, Mouse and Human.

Reviews using NB100-1028:
Filter by Applications
IF
(5)
ICC
(1)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(6)
Rat
(2)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence AIF-1/Iba1 NB100-1028
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reviewed by:
Anonymous
IF Mouse 04/23/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested Mouse brain
SpeciesMouse
LotS7G7G2P19
 AIF-1/Iba1 NB100-1028
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reviewed by:
Fuyi Chen
Mouse and Human 03/16/2018
View

Summary

Sample Tested Mouse brain
SpeciesMouse and Human
LotS7G7G2P15E031117
 
reviewed by:
Haiyan Qiu
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 09/22/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
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reviewed by:
Anonymous
IF Mouse 05/11/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
LotS7C7P6

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, RT

Primary Anitbody

Dilution Ratio1/500, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555
Secondary Manufacturer Cat#A21432
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Rat 10/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat frozen 4% paraformalehyde fixed brain sections
SpeciesRat
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
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reviewed by:
Xin Luo
IHC-P Mouse 06/30/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsTNB, 15 min, room temp

Primary Anitbody

Dilution Ratio1:20 in TNB, 60 min, room temp

Secondary Antibody

Secondary DescriptionDonkey anti-goat IgG, Cy5.5
Secondary Manufacturer Cat#NB120-6951
Secondary Concentration1:20

Details

Detection NotesEVOS microscope with APC cube
Fixation DetailsAntigen retrieval with citrate buffer, 98C for 30 min, cool 20 min
Wash DescriptionPBS, 2x of 4 min each
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Jaycie Loewen
IF Mouse 07/22/2013
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Grace zhou
IF Rat 07/16/2012
View

Summary

ApplicationImmunofluorescence
Sample Tested brain and spinal cord
SpeciesRat
Lots5c2g2

Blocking

Blocking DetailsPBS-T

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionANTI-GOAT
Secondary Manufacturer Cat#Jackson immunoresearch
Secondary Concentration1:1000

Details

Fixation Details4%PFA
Wash DescriptionPBS-T
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF 06/26/2012
View

Summary

ApplicationImmunofluorescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NB100-1028) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.6
5
7
4
2
3
1
2
0
1
0

Anonymous
04/23/2018
Application: IF
Species: Mouse

Fuyi Chen
03/16/2018
Application:
Species: Mouse and Human

 
Haiyan Qiu
04/01/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot