Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Human Lymph node (Molecular weight: 16.7 KDa) (A), Mouse Brain (B) and Rat Brain (C) lysate with AIF-1/Iba1 Antibody at 1 ug/mL (35 ug protein in RIPA ...read more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - A20 silencing prevents the anti-inflammatory effects of EA after focal cerebral ischemia reperfusion. Experiments were performed 24 h after ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining of paraffin embedded Mouse Lung using AIF-1/Iba1 Antibody. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining (1:500).
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). at 1:250. IHC image ...read more
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using. IHC image submitted by a verified customer review.
Immunohistochemistry Whole-Mount: AIF-1/Iba1 Antibody [NB100-1028] - Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining in rat hippocampus using AIF-1/Iba1 Antibody at 40X. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). AIF-1/Iba1 antibody validated for IHC-Whole mount, IHC-F from verified customer reviews. Dual ISH-IHC validation (PMID: 31220182).
16.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Expected from sequence similarity: Porcine. Mouse reactivity reported in scientific literature (PMID: 26490872). Canine, Feline reactivity reported from verified customer reviews.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AIF-1/Iba1 Antibody
allograft inflammatory factor 1
interferon gamma responsive transcript
Ionized calcium-binding adapter molecule 1
Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).
Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.
1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149
2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4
3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018
4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639
5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Morita A, Jullienne A, Salehi A et al. Temporal evolution of heme oxygenase-1 expression in reactive astrocytes and microglia in response to traumatic brain injury Brain Hemorrhages Feb 1 2020 (IHC, Mouse)
FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).
What is the volume of the sample size (NB100-1028SS)?
According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml. Therefore, the vial should contain 40 ul.
The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?
With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol:https://www.novusbio.com/support/support-by-application.html
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.