Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Mouse (A) and Rat (B) Brain lysate with antibody at 0.5 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - A20 silencing prevents the anti-inflammatory effects of EA after focal cerebral ischemia reperfusion. Experiments were performed 24 h after ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Reactive microglia (green) in peritumoral area of IC injected human glioblastoma cells in mouse brain. NeuN (red), DAPI (blue). Antibody at 1:250. ...read more
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC image submitted by a verified customer review.
Immunohistochemistry Whole-Mount: AIF-1/Iba1 Antibody [NB100-1028] - Mouse dorsal root ganglia. AIF-1/Iba1 expression in reactive macrophages. Maximum intensity z-projection from 3D images. IHC image submitted by a ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Feline optic nerve microglia were immunolabeled with AIF-1/Iba1 Antibody (green), DAPI (blue). Imaging was performed using an epifluorescence microscope. ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining in rat hippocampus using AIF-1/Iba1 Antibody. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Brown arrow= AIF-1/Iba1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and AIF-1/Iba1 positive cells. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain using AIF-1/Iba1 Antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Human glioma. PDIA3 staining in human GB specimens: the photograph shows a representative tumor field for PDIA3 and IBA1 (magnification: 40x). Double ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Canine liver stained for Iba1 (brown) and counterstained with Richard-Allan Scientific hematoxylin. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - AIF-1/Iba1 in canine brain. Primary antibody used at 1 ug/mL and incubated with tissue for 30 minutes at room temperature. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining of paraffin embedded Human Spleen with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
ICC/IF use reported in scientific literature (PMID: 26335282). Use in IHC-Whole mount reported in scientific literature (PMID: 26676316). This AIF-1/Iba1 antibody is validated for IHC-Whole mount and IHC-F from verified customer reviews. Dual ISH-IHC validation reported in scientific literature (PMID: 31220182).
16.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use in Rat reported in scientific literature (PMID: 33704579). Feline and canine reactivity reported from verified customer reviews.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AIF-1/Iba1 Antibody
allograft inflammatory factor 1
interferon gamma responsive transcript
Ionized calcium-binding adapter molecule 1
Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).
Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.
1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149
2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4
3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018
4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639
5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Shemer A, Grozovski J, Leng Tay T et al. Engrafted parenchymal brain macrophages differ from host microglia in transcriptome, epigenome and response to challenge bioRxiv Jul 16 2018 (IHC-Fr, Mouse)
Faust TE, Xin W, Lee B et al. Astrocyte redox dysregulation causes prefrontal hypoactivity: sulforaphane treats non-ictal pathophysiology in ALDH7A1-mediated epilepsy bioRxiv 2019 Oct 07 (IHC, Mouse)
McKinsey GL, Lizama CO, Keown-Lang AE, Niu A New Tools for Genetically Targeting Myeloid Populations in the Central Nervous System bioRxiv Dec 23 2019 (IHC, Mouse)
Boyles DA Determining the cellular targets and resulting pathology of rift valley fever virus infection of the rat CNS and reproductive system using microscopy Thesis Jan 1 2020
Morita A, Jullienne A, Salehi A et al. Temporal evolution of heme oxygenase-1 expression in reactive astrocytes and microglia in response to traumatic brain injury Brain Hemorrhages Feb 1 2020 (IHC, Mouse)
Ping S, Qiu X, Kyle M, Zhao LR Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke researchgate.net Jan 1 2020 (IHC, Mouse)
Thiesler H Polysialic acid and polysialic acid receptors as regulators of microglia and macrophage activation Thesis Jan 1 2020 (ICC/IF, Human)
Jiang L, Zhang Y, Jing F et al. P2X7R-mediated Autophagic Impairment Contributes to Central Sensitization in a chronic Migraine Model with Recurrent Nitroglycerin Stimulation in Mice Research Square Sep 3 2020 (ICC/IF, Mouse)
Zapata G Bptf is essential for murine neocortical development Thesis Jan 1 2020 (ICC/IF, Mouse)
Worthen RJ Regulation of Mouse Learning and Mood by the Anti-Inflammatory Cytokine Interleukin-10 Thesis Jan 1 2020
Xia D, Lianoglou S, Sandmann T, et al. Fibrillar A beta causes profound microglial metabolic perturbations in a novel APP knock-in mouse model bioRxiv Jan 20 2021 (ICC/IF, Mouse)
Following deparaffinization, pre-heated >=90C Target Retrieval Solution - Dako S1699 - was added to slides and then the slide container was placed into a vegetable steamer for 20min. Following antigen retrieval, slides were cooled for 15min, washed with 1X PBS, then placed in 3 percent hydrogen peroxide diluted in methanol. Following peroxide quench, slides were sequentially incubated - 10min each at room temperature with washes in between - in avidin and biotin solution -abcam ab64212- then Dako Serum-Free Protein Block - Dako X0909. After incubation with the protein block, slides were incubated with the primary antibody diluted 1:500 in Dako Antibody Diluent - Dako S3022 - for 30 min at room temperature. Slides were then incubated with rabbit anti-goat - Vector Labs BA5000 - diluted at 1:200 in Dako Serum-Free Protein Block - Dako X0909. Slides were then incubated with VECTASTAIN Elite ABC-HRP Reagent - Vector Labs PK-7100 - for 30min at room temperature. Staining was then developed using Dako DAB as a chromogen.
FAQs for AIF-1/Iba1 Antibody (NB100-1028). (Showing 1 - 2 of 2 FAQs).
What is the volume of the sample size (NB100-1028SS)?
According to our datasheet, NB100-1028SS is a package containing 0.02 mg, with the concentration of 0.5 mg/ml. Therefore, the vial should contain 40 ul.
The experimental method in the NB100-1028 instruction is not clear. We use frozen sections of brain tissue. But we can't produce satisfactory results. Could you provide us with a set of experimental methods in detail to help us get the results of the experiment?
With regards to this AIF-1/Iba1 Antibody NB100-1028, we unfortunately do not have any suggested protocol for this product. If you would like, you can please refer to our general protocol:https://www.novusbio.com/support/support-by-application.html
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