AIF-1/Iba1 Antibody

Images

 
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of human frontal cortex (A), Rat Brain (B), Mouse Brain (C), and Pig Brain (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by ...read more
Immunohistochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Staining in canine optic nerve.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Immunoreacitiy in a mouse spinal cord after focal injection of lysolecitithin into the ventral column white matter. Image supplied by a certified customer ...read more
Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain. Image supplied by a certified customer review.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Mouse spinal cord microglia staining in frozen sections. Image supplied by a certified customer review.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining in rat hippocampus at 40x. Image supplied by a certified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Staining in mouse brain. Image supplied by a certified customer review.
Immunohistochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Staining in Mouse brain. Image supplied by a certified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-FrFl, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF1 / IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection Limit 1:128000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
Application Notes
WB: ~17 kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). IHC: Use reported in scientific literature and customer reviews. However, variation betwen lots has been observed. ICC: Use reported in scientific literature (PMID: 26335282). Use in Immunohistochemistry, Western blot, Immunohistochemistry frozen, Immunohistochemistry-free floating, Immunohistochemistry-paraffin, and Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 26676316). Please contact technical support if you have any questions.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AIF-1/Iba1 Lysate (NBL1-07408)
Reviewed Applications
Read 12 Reviews rated 4.6
using
NB100-1028 in the following applications:

Publications
Read Publications using
NB100-1028 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26490872). Canine reactivity per customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • AIF-1IRT1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IBA1Em:AF129756.17
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-683
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NB100-1028
Species: Hu, Mu, Rt, Po, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-FrFl, IHC-WhMt

Publications for AIF-1/Iba1 Antibody (NB100-1028)(72)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(15)
IHC
(13)
IHC-FR
(1)
IHC-Fr
(7)
IHC-FrFl
(3)
IHC-P
(4)
IHC-WhMt
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(30)
Rat
(14)
All Species
Showing Publications 1 - 10 of 72. Show All 72 Publications.
Publications using NB100-1028 Applications Species
Mathew B, Ravindran S, Liu X et al. Mesenchymal stem cell-derived extracellular vesicles and retinal ischemia-reperfusion. Biomaterials Jan 9 2019 [PMID: 30654160] (ICC/IF, Human) ICC/IF Human
Abraham D, Feher J, Scuderi GL et al. Exercise and probiotics attenuate the development of Alzheimer's disease in transgenic mice: Role of microbiome Exp. Gerontol. Dec 6 2018 [PMID: 30529024] (IHC, Mouse) IHC Mouse
Sprenkle NT, Lahiri A, Simpkins JW, Meares GP. Endoplasmic reticulum stress is transmissible in vitro between cells of the central nervous system J. Neurochem. Dec 6 2018 [PMID: 30520047] (WB, Mouse) WB Mouse
Rivera PD, Hanamsagar R, Kan MJ et al. Removal of microglial-specific MyD88 signaling alters dentate gyrus doublecortin and enhances opioid addiction-like behaviors. Brain Behav. Immun. Nov 15 2018 [PMID: 30447281] (IHC, Mouse) IHC Mouse
Mao SPH, Park M, Cabrera RM et al. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res. Oct 26 2018 [PMID: 30367629] (ICC/IF, Mouse) ICC/IF Mouse
Zhang C, Lai MB, Pedler MG et al. Endothelial Cell-Specific Inactivation of TSPAN12 (Tetraspanin 12) Reveals Pathological Consequences of Barrier Defects in an Otherwise Intact Vasculature. Arterioscler. Thromb. Vasc. Biol. Nov 1 2018 [PMID: 30354230] (ICC/IF, Mouse) ICC/IF Mouse
Polis B, Srikanth KD, Elliott E et al. L-Norvaline Reverses Cognitive Decline and Synaptic Loss in a Murine Model of Alzheimer's Disease Neurotherapeutics Oct 1 2018 [PMID: 30288668] (IHC-P, Mouse) IHC-P Mouse
Bussian TJ, Aziz A, Meyer CF et al. Clearance of senescent glial cells prevents tau-dependent pathology and cognitive decline. Nature Sep 19 2018 [PMID: 30232451] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Xu H, Mu S, Qin W. Microglia TREM2 is required for electroacupuncture to attenuate neuroinflammation in focal cerebral ischemia/reperfusion rats Biochem. Biophys. Res. Commun. Aug 24 2018 [PMID: 30149915] (ICC/IF, Rat) ICC/IF Rat
Lenz KM, Pickett LA, Wright CL et al. Mast Cells in the Developing Brain Determine Adult Sexual Behavior. bioRxiv. [PMID: 30093566] (IHC, Rat) IHC Rat
Show All 72 Publications.

Reviews for AIF-1/Iba1 Antibody (NB100-1028) (12) 4.612

Average Rating: 4.6
(Based on 12 reviews)
We have 12 reviews tested in 4 species: Mouse, Rat, Canine, Mouse and Human.

Reviews using NB100-1028:
Filter by Applications
IHC-Fr
(3)
IHC-P
(2)
IF
(5)
ICC
(1)
All Applications
Filter by Species
Mouse
(7)
Rat
(2)
Canine
(1)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
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reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotS7G7G2P19
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-P Canine 08/07/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOptic nerve
SpeciesCanine
LotS7C7G2P15
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF Mouse 04/23/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested Mouse brain
SpeciesMouse
LotS7G7G2P19
 AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Fuyi Chen
Mouse and Human 03/16/2018
View

Summary

Sample Tested Mouse brain
SpeciesMouse and Human
LotS7G7G2P15E031117
 
reviewed by:
Haiyan Qiu
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 09/22/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord
SpeciesMouse
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF Mouse 05/11/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse spinal cord
SpeciesMouse
LotS7C7P6

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, RT

Primary Anitbody

Dilution Ratio1/500, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555
Secondary Manufacturer Cat#A21432
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS
Immunohistochemistry-Frozen AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IHC-Fr Rat 10/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat frozen 4% paraformalehyde fixed brain sections
SpeciesRat
Immunohistochemistry-Paraffin AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Xin Luo
IHC-P Mouse 06/30/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking DetailsTNB, 15 min, room temp

Primary Anitbody

Dilution Ratio1:20 in TNB, 60 min, room temp

Secondary Antibody

Secondary DescriptionDonkey anti-goat IgG, Cy5.5
Secondary Manufacturer Cat#NB120-6951
Secondary Concentration1:20

Details

Detection NotesEVOS microscope with APC cube
Fixation DetailsAntigen retrieval with citrate buffer, 98C for 30 min, cool 20 min
Wash DescriptionPBS, 2x of 4 min each
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Jaycie Loewen
IF Mouse 07/22/2013
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunofluorescence AIF-1/Iba1 NB100-1028
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reviewed by:
Grace zhou
IF Rat 07/16/2012
View

Summary

ApplicationImmunofluorescence
Sample Tested brain and spinal cord
SpeciesRat
Lots5c2g2

Blocking

Blocking DetailsPBS-T

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionANTI-GOAT
Secondary Manufacturer Cat#Jackson immunoresearch
Secondary Concentration1:1000

Details

Fixation Details4%PFA
Wash DescriptionPBS-T
Immunofluorescence AIF-1/Iba1 NB100-1028
Enlarge
reviewed by:
Anonymous
IF 06/26/2012
View

Summary

ApplicationImmunofluorescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NB100-1028) (0)

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Secondary Antibodies

 

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Recent Reviews

4.6
5
8
4
3
3
1
2
0
1
0

Kevin Thorburn
11/29/2018
Application: IHC-Fr
Species: Mouse

Anonymous
08/07/2018
Application: IHC-P
Species: Canine

Anonymous
04/23/2018
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot