Immunocytochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Analysis of Iba1 in rat brain and spinal cord. Image courtesy of anonymous customer product review.
Immunocytochemistry: AIF-1/Iba1 Antibody [NB100-1028] - Analysis of Iba1 in rat samples. Image courtesy of product review submitted by Tatyana Kanyshkova.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Iba-1 in mouse brain. Image from verified customer review.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB100-1028] - Staining of frozen rat hippocampal tissue with AIF-1 antibody. Image from verified customer review.
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of human (A), mouse (B), and rat (C) brain lysates (35 ug protein in RIPA buffer). Primary antibody incubation for 1 hour at RT. Detected by chemiluminescence
Peptide with sequence C-TGPPAKKAISELP corresponding to C-Terminus according to NP_001614.3.
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF1 / IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Porcine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Western blot: Approx 18 kDa band observed in human brain (frontal cortex), 17 kDa in mouse brain and 16 kDa in rat brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3, and 16.8 kDa according to Mouse NP_062340.1 and Rat NP_058892.1). Primary incubation 1 hour at room temperature.
17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 26490872).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AIF-1/Iba1 Antibody
allograft inflammatory factor 1
interferon gamma responsive transcript
Ionized calcium-binding adapter molecule 1
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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