Abhd5 Antibody - BSA Free

Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license.

Western Blot: Abhd5 Antibody [NB110-41576] - Abhd5 overexpression lysates (NBL1-07199).

Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.

Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Protein content of important players involved in lipid droplet dynamics in Caucasian and South Asian subjects before and after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 and (e) PLIN5 protein content. Data are presented as mean ± SEM and were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect.

The turnover of DAG and TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) and ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - A. ATGL (n = 8) & CGI-58 (n = 7) protein content in the three skeletal muscles (SOL, RG, & WG & heart).Ponceau stain for total protein was used as a loading control. Data are reported as mean±SE & bars with the same letter are not significantly different within the skeletal muscles (p<0.05). Insets: representative blots: lane 1, WG (white gastrocnemius); lane 2, RG (red gastrocnemius); lane 3, SOL (soleus). B. ATGL & CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - The turnover of DAG & TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) & ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the right ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Protein content of important players involved in lipid droplet dynamics in Caucasian & South Asian subjects before & after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 & (e) PLIN5 protein content. Data are presented as mean ± SEM & were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the left ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), G0/G1 switch gene 2 (G0S2), hormone sensitive lipase (HSL). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Abhd5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Abhd5 Antibody - BSA Free (NB110-41576) is a polyclonal antibody validated for use in WB and Simple Western. Anti-Abhd5 Antibody: Cited in 29 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region within residues 200-300 of the human Abhd5 protein. [Swiss-Prot# Q8WTS1]
• Localization: Cytoplasm. Colocalizatd with PLIN and ADRP on the surface of lipid droplets. The localization is dependant upon the metabolic status of the adipocytes and the activity of PKA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABHD5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:1000
  • Western Blot 1:500
• Application Notes: A band is seen at ~43 kDa in Western Blot. In ICC/IF cytoplasmic staining was observed in HeLa cells.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 55 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  Abhd5 Overexpression Lysate

• Control Peptide:

  Abhd5 Peptide (NB110-41576PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:23878361)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Abhd5 Antibody - BSA Free

• abhydrolase domain containing 5
• Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• CDS
• CGI58
• EC 2.3.1.51
• IECN2
• Lipid droplet-binding protein CGI-58
• MGC8731
• NCIE2CGI-58

Background

ABHD5 (abhydrolase domain containing protein 5; or lipid droplet-binding protein CGI-58) belongs to peptidase S33 family and it is a ubiquitously expressed enzyme which is critical to triacylglycerol (TAG) metabolism. Localized in the cytoplasm and lipid droplets, ABHD5 colocalized with PLIN/ADRP on lipid droplets surfaces. It is widely expressed in various tissues including lymphocytes, liver, skeletal muscle and brain. Biochemically, ABHD5 is a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis and regulate the cellular storage of TAG through activation of phospholipase PNPLA2. ABHD5 mutations have been linked to neutral lipid storage disorder called Chanarin-Dorfman syndrome which is characterized by ichthyosis, hepatic steatosis, and excessive storage of TAG in multiple cells and tissues. ABHD5 lacks TAG hydrolase activity, but increases the lipase activity of ATGL through a poorly understood mechanism. In vitro and in vivo studies established that modulating expression of ABHD5 significantly alters lipolysis and levels of TAG, and endogenous ABHD5 expression are limiting in murine adipose tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Abhd5 Antibody (NB110-41576)(29)

Publications using NB110-41576

• Sundararajan Mahalingam, Ramesh Bellamkonda, Kusum K Kharbanda, Madan Kumar Arumugam, Vikas Kumar, Carol A Casey, Lorenzo Leggio, Karuna Rasineni Role of ghrelin hormone in the development of alcohol-associated liver disease. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024-04-18 [PMID: 38640709]
• Mahalingam S, Bellamkonda R, Arumugam MK et al. Glucagon-like peptide 1 receptor agonist, exendin-4, reduces alcohol-associated fatty liver disease Biochemical pharmacology 2023-05-18 [PMID: 37209859] (WB, Rat)
• Bao X, Ma X, Huang R et al. Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice Journal of Molecular Cell Biology 2022-12-26 [PMID: 36107452]
• Chrzanowski-Smith OJ, Edinburgh RM, Smith E et al. Resting skeletal muscle PNPLA2 (ATGL) and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences Experimental physiology 2021-03-06 [PMID: 33675111] (WB, Human)
• Fievet A, Bellanger D, Rieunier G et al. Assessment of the Main Compounds of the Lipolytic System in Treadmill Running Rats: Different Response Patterns between the Right and Left Ventricle Int J Mol Sci 2019-05-24 [PMID: 31137663] (WB, Rat)
• Daemen S, Gemmink A, Brouwers B et al. Distinct lipid droplet characteristics and distribution unmask the apparent contradiction of the athlete's paradox Molecular Metabolism 2018-08-01 [PMID: 30174227] (WB, Human)
• Rogne M, Chu DT, Kuntziger TM et al. OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells. Mol. Biol. Cell 2018-04-24 [PMID: 29688805] (Human)
• Perreault L, Newsom SA, Strauss A, Kerege A. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle. JCI Insight. 2018-02-08 [PMID: 29415895] (WB, Human)
• Snook LA, Trottier SK, Worndl EA et al. Prior Endurance Training Enhances Beta-Adrenergic Signaling in Epidydimal Adipose from Mice Fed a High-Fat Diet Obesity (Silver Spring) 2017-10-01 [PMID: 28857453] (Mouse)
• Gemmink A, Bakker LE, Guigas B et al. Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians. Sci Rep. 2017-02-14 [PMID: 28195217] (WB, Human)
• Turnbull PC, Longo AB, Ramos SV et al. Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2015-10-28 [PMID: 26511521] (WB, Rat)
• Turnbull PC, Ramos SV, MacPherson RE et al. Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58). PLoS One 2015-01-01 [PMID: 25811590] (WB, Rat)
• Newsom SA, Everett AC, Park S et al. Lipid mixtures containing a very high proportion of saturated Fatty acids only modestly impair insulin signaling in cultured muscle cells. PLoS ONE. 2015-03-21 [PMID: 25793412] (WB, Mouse)
• MacPherson RE, Castelli LM, Miotto PM et al. A Maternal High Fat Diet Has Long-Lasting Effects on Skeletal Muscle Lipid and PLIN Protein Content in Rat Offspring at Young Adulthood. Lipids. 2015-01-01 [PMID: 25552350]
• Xie P, Zeng X, Xiao J et al. Transgenic CGI-58 expression in macrophages alleviates the atherosclerotic lesion development in ApoE knockout mice. Biochim. Biophys. Acta. 2014-08-29 [PMID: 25178844] (WB)
• Mowers J, Uhm M, Reilly SM et al. Inflammation produces catecholamine resistance in obesity via activation of PDE3B by the protein kinases IKKepsilon and TBK1. Elife 2013-12-24 [PMID: 24368730] (WB, Mouse)
• Macpherson RE, Vandenboom R, Roy BD, Peters SJ. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physiol Rep. 2013-09-01 [PMID: 24303154] (WB, Rat)
• Macdonald TL, Wan Z, Frendo-Cumbo S et al. IL-6 and epinephrine have divergent fiber type effects on intramuscular lipolysis. J Appl Physiol (1985). 2013-11-01 [PMID: 24052031] (WB, Mouse)
• Alsted TJ, Ploug T, Prats C et al. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. J Physiol 2013-10-15 [PMID: 23878361] (WB, Mouse)
• Sitnick MT, Basantani MK, Cai L et al. Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity. Diabetes 2013-10-01 [PMID: 23835334] (Mouse)
• Macpherson RE, Ramos S, Vandenboom R et al. Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction. Am J Physiol Regul Integr Comp Physiol 2013-02-13 [PMID: 23408028] (WB, Rat)
• Bosma M, Sparks LM, Hooiveld G et al. Overexpression of PLIN5 in skeletal muscle promotes oxidative gene expression and intramyocellular lipid content without compromising insulin sensitivity. Biochim Biophys Acta 2013-01-22 [PMID: 23353597] (WB, Rat)
• Koves TR, Sparks LM, Kovalik JP et al. PPAR coactivator-1alpha contributes to exercise-induced regulation of intramuscular lipid droplet programming in mice and humans. J Lipid Res 2013-02-01 [PMID: 23175776]
• Li X, Suh Y, Kim E et al. Alternative splicing and developmental and hormonal regulation of porcine comparative gene identification-58 (CGI-58) mRNA. J Anim Sci 2012-12-01 [PMID: 22829614]
• Bosma M, Hesselink MK, Sparks LM et al. Perilipin 2 improves insulin sensitivity in skeletal muscle despite elevated intramuscular lipid levels Diabetes 2012-11-01 [PMID: 22807032]
• Goossens GH, Moors CC, van der Zijl NJ et al. Valsartan Improves Adipose Tissue Function in Humans with Impaired Glucose Metabolism: A Randomized Placebo-Controlled Double-Blind Trial PLoS One 2012-01-01 [PMID: 22768174] (WB, Human)
• Timmers S, de Vogel-van den Bosch J, Hesselink M et al. Paradoxical Increase in TAG and DAG Content Parallel the Insulin Sensitizing Effect of Unilateral DGAT1 Overexpression in Rat Skeletal Muscle. PLoS ONE January 2011, Volume 6,Issue 1. [PMID: 21264296]
• Jocken JW, Moro C, Goossens GH et al. Skeletal Muscle Lipase Content and Activity in Obesity and Type 2 Diabetes. J Clin Endocrinol Metab. 2010-09-15 [PMID: 20843949]
• Alsted TJ, Nybo L, Schweiger M et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training. Am J Physiol Endocrinol Metab;296(3):E445-453. 2009-01-01 [PMID: 19106247]

Bioinformatics

• Gene Symbol: ABHD5