Abhd5 Antibody

Images

 
Western Blot: Abhd5 Antibody [NB110-41576] - Western Blot on Abhd5 overexpression lysates (NBL1-07199).
Western Blot: Abhd5 Antibody [NB110-41576] - Western Blot on MCF7 cell lysates.
Simple Western: Abhd5 Antibody [NB110-41576] - Simple Western lane view shows a specific band for Abhd5 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Abhd5 Antibody Summary

Immunogen
A synthetic peptide made to an internal region within residues 200-300 of the human Abhd5 protein. [Swiss-Prot# Q8WTS1]
Localization
Cytoplasm. Colocalizatd with PLIN and ADRP on the surface of lipid droplets. The localization is dependant upon the metabolic status of the adipocytes and the activity of PKA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABHD5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:1000
Application Notes
This Abhd5 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis, where a band is seen at ~43 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Skeletal Muscle Lysate (NB820-59253)
Abhd5 Lysate (NBL1-07199)
Control Peptide
Abhd5 Peptide (NB110-41576PEP)
Publications
Read Publications using
NB110-41576 in the following applications:

  • WB
    14 publications

Reactivity Notes

Human and rat. Mouse reactivity reported in scientific literature (PMID:23878361)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
IgG purified

Alternate Names for Abhd5 Antibody

  • abhydrolase domain containing 5
  • Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
  • CDS
  • CGI58
  • EC 2.3.1.51
  • IECN2
  • Lipid droplet-binding protein CGI-58
  • MGC8731
  • NCIE2CGI-58

Background

ABHD5 (abhydrolase domain containing protein 5; or lipid droplet-binding protein CGI-58) belongs to peptidase S33 family and it is a ubiquitously expressed enzyme which is critical to triacylglycerol (TAG) metabolism. Localized in the cytoplasm and lipid droplets, ABHD5 colocalized with PLIN/ADRP on lipid droplets surfaces. It is widely expressed in various tissues including lymphocytes, liver, skeletal muscle and brain. Biochemically, ABHD5 is a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis and regulate the cellular storage of TAG through activation of phospholipase PNPLA2. ABHD5 mutations have been linked to neutral lipid storage disorder called Chanarin-Dorfman syndrome which is characterized by ichthyosis, hepatic steatosis, and excessive storage of TAG in multiple cells and tissues. ABHD5 lacks TAG hydrolase activity, but increases the lipase activity of ATGL through a poorly understood mechanism. In vitro and in vivo studies established that modulating expression of ABHD5 significantly alters lipolysis and levels of TAG, and endogenous ABHD5 expression are limiting in murine adipose tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Abhd5 Antibody (NB110-41576)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


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WB
(14)
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Human
(4)
Mouse
(6)
Rat
(6)
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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB110-41576 Applications Species
Rogne M, Chu DT, Kuntziger TM et al. OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells. Mol. Biol. Cell Apr 24 2018 [PMID: 29688805] (Human) Human
MacPherson REK et al. The role of skeletal muscle PLIN proteins at rest and following lipolytic stimulation. Thesis, Brock University 2013 Dec 16 (WB, Rat) WB Rat
Perreault L, Newsom SA, Strauss A, Kerege A. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle. JCI Insight. 2018 Feb 08 [PMID: 29415895] (WB, Human) WB Human
Snook LA, Trottier SK, Worndl EA et al. Prior Endurance Training Enhances Beta-Adrenergic Signaling in Epidydimal Adipose from Mice Fed a High-Fat Diet Obesity (Silver Spring) 2017 Oct 01 [PMID: 28857453] (Mouse) Mouse
Gemmink A, Bakker LE, Guigas B et al. Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians. Sci Rep. 2017 Feb 14 [PMID: 28195217] (WB, Human) WB Human
Turnbull PC, Longo AB, Ramos SV et al. Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2015 Oct 28 [PMID: 26511521] (WB, Rat) WB Rat
Turnbull PC, Ramos SV, MacPherson RE et al. Characterization of Lipolytic Inhibitor G(0)/G(1) Switch Gene-2 Protein (G0S2) Expression in Male Sprague-Dawley Rat Skeletal Muscle Compared to Relative Content of Adipose Triglyceride Lipase (ATGL) and Comparitive Gene Identification-58 (CGI-58) PLoS ONE 2015 Mar 27 [PMID: 25811590] (WB, Rat) WB Rat
Newsom SA, Everett AC, Park S et al. Lipid mixtures containing a very high proportion of saturated Fatty acids only modestly impair insulin signaling in cultured muscle cells. PLoS ONE. 2015 Mar 21 [PMID: 25793412] (WB, Mouse) WB Mouse
MacPherson RE, Castelli LM, Miotto PM et al. A Maternal High Fat Diet Has Long-Lasting Effects on Skeletal Muscle Lipid and PLIN Protein Content in Rat Offspring at Young Adulthood. Lipids. 2015 Jan 01 [PMID: 25552350]
Xie P, Zeng X, Xiao J et al. Transgenic CGI-58 expression in macrophages alleviates the atherosclerotic lesion development in ApoE knockout mice. Biochim. Biophys. Acta. 2014 Aug 29 [PMID: 25178844] (WB) WB
Show All 24 Publications.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ABHD5