Abhd5 Antibody


Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication ...read more
Western Blot: Abhd5 Antibody [NB110-41576] - Abhd5 overexpression lysates (NBL1-07199).
Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ...read more
Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.
Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
1.0 mg/ml

Abhd5 Antibody Summary

A synthetic peptide made to an internal region within residues 200-300 of the human Abhd5 protein. [Swiss-Prot# Q8WTS1]
Cytoplasm. Colocalizatd with PLIN and ADRP on the surface of lipid droplets. The localization is dependant upon the metabolic status of the adipocytes and the activity of PKA.
Immunogen affinity purified
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  • Western Blot 1:500
  • Simple Western 1:1000
Application Notes
In Western blot analysis, where a band is seen at ~43 kDa. In ICC/IF cytoplasmic staining was observed in HeLa cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Abhd5 Overexpression Lysate
Control Peptide
Abhd5 Peptide (NB110-41576PEP)
Read Publications using
NB110-41576 in the following applications:

  • WB
    15 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:23878361)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Abhd5 Antibody

  • abhydrolase domain containing 5
  • Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
  • CDS
  • CGI58
  • EC
  • IECN2
  • Lipid droplet-binding protein CGI-58
  • MGC8731
  • NCIE2CGI-58


ABHD5 (abhydrolase domain containing protein 5; or lipid droplet-binding protein CGI-58) belongs to peptidase S33 family and it is a ubiquitously expressed enzyme which is critical to triacylglycerol (TAG) metabolism. Localized in the cytoplasm and lipid droplets, ABHD5 colocalized with PLIN/ADRP on lipid droplets surfaces. It is widely expressed in various tissues including lymphocytes, liver, skeletal muscle and brain. Biochemically, ABHD5 is a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis and regulate the cellular storage of TAG through activation of phospholipase PNPLA2. ABHD5 mutations have been linked to neutral lipid storage disorder called Chanarin-Dorfman syndrome which is characterized by ichthyosis, hepatic steatosis, and excessive storage of TAG in multiple cells and tissues. ABHD5 lacks TAG hydrolase activity, but increases the lipase activity of ATGL through a poorly understood mechanism. In vitro and in vivo studies established that modulating expression of ABHD5 significantly alters lipolysis and levels of TAG, and endogenous ABHD5 expression are limiting in murine adipose tissue.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Abhd5 Antibody (NB110-41576)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB110-41576 Applications Species
Fievet A, Bellanger D, Rieunier G et al. Assessment of the Main Compounds of the Lipolytic System in Treadmill Running Rats: Different Response Patterns between the Right and Left Ventricle Int J Mol Sci May 24 2019 [PMID: 31137663] (WB, Rat) WB Rat
Daemen S, Gemmink A, Brouwers B et al. Distinct lipid droplet characteristics and distribution unmask the apparent contradiction of the athlete's paradox Molecular Metabolism Aug 1 2018 [PMID: 30174227] (WB, Human) WB Human
Rogne M, Chu DT, Kuntziger TM et al. OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells. Mol. Biol. Cell Apr 24 2018 [PMID: 29688805] (Human) Human
Perreault L, Newsom SA, Strauss A, Kerege A. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle. JCI Insight. 2018 Feb 08 [PMID: 29415895] (WB, Human) WB Human
Snook LA, Trottier SK, Worndl EA et al. Prior Endurance Training Enhances Beta-Adrenergic Signaling in Epidydimal Adipose from Mice Fed a High-Fat Diet Obesity (Silver Spring) 2017 Oct 01 [PMID: 28857453] (Mouse) Mouse
Gemmink A, Bakker LE, Guigas B et al. Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians. Sci Rep. 2017 Feb 14 [PMID: 28195217] (WB, Human) WB Human
Turnbull PC, Longo AB, Ramos SV et al. Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2015 Oct 28 [PMID: 26511521] (WB, Rat) WB Rat
Turnbull PC, Ramos SV, MacPherson RE et al. Characterization of Lipolytic Inhibitor G(0)/G(1) Switch Gene-2 Protein (G0S2) Expression in Male Sprague-Dawley Rat Skeletal Muscle Compared to Relative Content of Adipose Triglyceride Lipase (ATGL) and Comparitive Gene Identification-58 (CGI-58) PLoS ONE 2015 Mar 27 [PMID: 25811590] (WB, Rat) WB Rat
Newsom SA, Everett AC, Park S et al. Lipid mixtures containing a very high proportion of saturated Fatty acids only modestly impair insulin signaling in cultured muscle cells. PLoS ONE. 2015 Mar 21 [PMID: 25793412] (WB, Mouse) WB Mouse
MacPherson RE, Castelli LM, Miotto PM et al. A Maternal High Fat Diet Has Long-Lasting Effects on Skeletal Muscle Lipid and PLIN Protein Content in Rat Offspring at Young Adulthood. Lipids. 2015 Jan 01 [PMID: 25552350]
Show All 25 Publications.

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Product General Protocols

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WB Video Protocol

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-41576AF405
Alexa Fluor 488 NB110-41576AF488
Alexa Fluor 647 NB110-41576AF647
Alexa Fluor 700 NB110-41576AF700
Biotin NB110-41576B
DyLight 488 NB110-41576G
DyLight 550 NB110-41576R
DyLight 650 NB110-41576C
HRP NB110-41576H

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Gene Symbol ABHD5