Lipase A Antibody

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Biological Strategies: Western Blot: Lipase A Antibody [NBP1-54155] - Western blot analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell ...read more
Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP1-54155] - ICC staining of LIPA in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: Lipase A Antibody [NBP1-54155] - IHC staining of LIPA in mouse stomach smooth muscle.
Western Blot: Lipase A Antibody [NBP1-54155] - WB detection of LIPA in HepG2 whole cell extract.
Simple Western: Lipase A Antibody [NBP1-54155] - Simple Western lane view shows a specific band for Lipase A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Lipase A Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human Lipase A protein (within residues 150-300). [Swiss-Prot P38571]
Localization
Lysosome.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:10000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This LIPA antibody is useful for ICC/IF, IHC-P and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-54155 in the following applications:

Publications
Read Publication using
NBP1-54155 in the following applications:

Reactivity Notes

Human and mouse. Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lipase A Antibody

  • Acid cholesteryl ester hydrolase
  • Cholesteryl esterase
  • EC 3.1.1
  • EC 3.1.1.13
  • LALCESDcholesterol ester hydrolase
  • Lipase A
  • lipase A, lysosomal acid, cholesterol esterase
  • lysosomal acid lipase
  • lysosomal acid lipase/cholesteryl ester hydrolase
  • Sterol esterase

Background

Lipase A (LIPA) is an enzyme essential for biotransformation, sequestration and breakdown of cholesterol. It is localized in lysosomes and is expressed at high levels in small intestinal villi, the zona fasciculate/reticularis of the adrenal cortex, pancreatic acini, renal tubular epithelium, hepatocytes, spleenocytes and thymic cells. LIPA is highly crucial for hydrolysis of cholesteryl esters and triglycerides internalized via receptor-mediated endocytosis of lipoproteins. It is also implicated in mediating LDL uptake effects on suppression of HMG-CoA reductase and activation of endogenous cholesteryl ester synthesis. Defective LIPA in liver and kidney may lead to lipoprotein cholesterol accumulation which results in pathologies including atherosclerosis, inflammation, NIDDM and chronic kidney disease. Defects in LIPA are the cause of Wolman disease (WOD) and cholesteryl ester storage disease (CESD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-54155
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Lipase A Antibody (NBP1-54155)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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Review for Lipase A Antibody (NBP1-54155) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-54155:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Lipase A NBP1-54155
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reviewed by:
Anonymous
WB Human 02/01/2018
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Summary

ApplicationWestern Blot
Sample Testedprostate adenoma tissue,HFL1 primary human fibroblasts extract
SpeciesHuman
LotA-4

Comments

CommentsPositive control: Human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell line lysates
Negative control: HFL1 primary human fibroblasts extract
20µg/lane protein
Incubate with primary rabbit anti-LIPA primary antibody (Novus Biologicals, NBP1-54155, lot A-4) diluted at 1:5000 in 3% TBS-T/BSA ON at 4°C.
Incubate with secondary goat anti-rabbit/HRP-conjugated antibody (Bio-Rad, cat 1706515) diluted 1:3000 in 3% TBS-T/BSA for 1h-2h at RT.
Image acquisition 30 sec exposure
Strip membrane
Incubate membrane with primary mouse beta-Actin loading control antibody (Novus Biologicals, NB600501, lot 64M4789V) diluted at 1:5000 in 3% TBS-T/BSA and with secondary anti-mouse/HRP-conjugated antibody (Bio-Rad, 170547) at 1:3000 in 3% TBS-T/BSA.
Image acquisition 30 sec exposure
Experimental notes & Conclusion:
NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45kDa in different lysates of human cancer cell lines. The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein.
Conclusion
Passed. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.

Validation lab name:
Giannis Lamprou, Avgi Tsolou, Michael Koukourakis; Department of Radiation Oncology - Radiobiology - Radiopathology, Medical School - Democritus University of Thrace - University Hospital of Alexandroupolis

Via antibodies-online Validation Program.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lipase A Antibody (NBP1-54155) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Lipase A.

How do Lipase A and the CD36 Antibody Relate to Each Other
Obesity, diabetes and metabolic disorders are dramatically on the increase, linked to disorders such as heart disease, stroke and cancer. To combat this, research groups are studying metabolism at both a cellular and a systemic level. Although we at N...  Read full blog post.

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Recent Reviews

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1
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Anonymous
02/01/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol LIPA
Entrez
Uniprot