Western Blot: Lipase A Antibody [NBP1-54155] - WB detection of LIPA in HepG2 whole cell extract.
Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP1-54155] - ICC staining of LIPA in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: Lipase A Antibody [NBP1-54155] - IHC staining of LIPA in mouse stomach smooth muscle.
Simple Western: Lipase A Antibody [NBP1-54155] - Simple Western lane view shows a specific band for Lipase A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This LIPA antibody is useful for ICC/IF, IHC-P and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Human and mouse. Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for Lipase A Antibody
Acid cholesteryl ester hydrolase
LALCESDcholesterol ester hydrolase
lipase A, lysosomal acid, cholesterol esterase
lysosomal acid lipase
lysosomal acid lipase/cholesteryl ester hydrolase
Lipase A (LIPA) is an enzyme essential for biotransformation, sequestration and breakdown of cholesterol. It is localized in lysosomes and is expressed at high levels in small intestinal villi, the zona fasciculate/reticularis of the adrenal cortex, pancreatic acini, renal tubular epithelium, hepatocytes, spleenocytes and thymic cells. LIPA is highly crucial for hydrolysis of cholesteryl esters and triglycerides internalized via receptor-mediated endocytosis of lipoproteins. It is also implicated in mediating LDL uptake effects on suppression of HMG-CoA reductase and activation of endogenous cholesteryl ester synthesis. Defective LIPA in liver and kidney may lead to lipoprotein cholesterol accumulation which results in pathologies including atherosclerosis, inflammation, NIDDM and chronic kidney disease. Defects in LIPA are the cause of Wolman disease (WOD) and cholesteryl ester storage disease (CESD).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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