Biological Strategies: Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 ...read more
Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP1-54155] - ICC staining of LIPA in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.
Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.
Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
This LIPA antibody is useful for ICC/IF, IHC-P and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, 50% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for Lipase A Antibody
Acid cholesteryl ester hydrolase
LALCESDcholesterol ester hydrolase
lipase A, lysosomal acid, cholesterol esterase
lysosomal acid lipase
lysosomal acid lipase/cholesteryl ester hydrolase
Lipase A (LIPA) is an enzyme essential for biotransformation, sequestration and breakdown of cholesterol. It is localized in lysosomes and is expressed at high levels in small intestinal villi, the zona fasciculate/reticularis of the adrenal cortex, pancreatic acini, renal tubular epithelium, hepatocytes, spleenocytes and thymic cells. LIPA is highly crucial for hydrolysis of cholesteryl esters and triglycerides internalized via receptor-mediated endocytosis of lipoproteins. It is also implicated in mediating LDL uptake effects on suppression of HMG-CoA reductase and activation of endogenous cholesteryl ester synthesis. Defective LIPA in liver and kidney may lead to lipoprotein cholesterol accumulation which results in pathologies including atherosclerosis, inflammation, NIDDM and chronic kidney disease. Defects in LIPA are the cause of Wolman disease (WOD) and cholesteryl ester storage disease (CESD).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
prostate adenoma tissue,HFL1 primary human fibroblasts extract
Positive control: Human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell line lysates Negative control: HFL1 primary human fibroblasts extract 20µg/lane protein Incubate with primary rabbit anti-LIPA primary antibody (Novus Biologicals, NBP1-54155, lot A-4) diluted at 1:5000 in 3% TBS-T/BSA ON at 4°C. Incubate with secondary goat anti-rabbit/HRP-conjugated antibody (Bio-Rad, cat 1706515) diluted 1:3000 in 3% TBS-T/BSA for 1h-2h at RT. Image acquisition 30 sec exposure Strip membrane Incubate membrane with primary mouse beta-Actin loading control antibody (Novus Biologicals, NB600501, lot 64M4789V) diluted at 1:5000 in 3% TBS-T/BSA and with secondary anti-mouse/HRP-conjugated antibody (Bio-Rad, 170547) at 1:3000 in 3% TBS-T/BSA. Image acquisition 30 sec exposure Experimental notes & Conclusion: NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45kDa in different lysates of human cancer cell lines. The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Conclusion Passed. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.
Validation lab name: Giannis Lamprou, Avgi Tsolou, Michael Koukourakis; Department of Radiation Oncology - Radiobiology - Radiopathology, Medical School - Democritus University of Thrace - University Hospital of Alexandroupolis
Via antibodies-online Validation Program.
Product General Protocols
View specific protocols for Lipase A Antibody (NBP1-54155):
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