A20/TNFAIP3 Antibody - BSA Free

Western Blot: A20/TNFAIP3 Antibody [NBP1-77024] - Analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody [NBP1-77024] - Human lung tissue with TNFAIP3 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] - Immunofluorescence of A20/TNFAIP3 in Human Lung cells with A20/TNFAIP3 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

A20/TNFAIP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit A20/TNFAIP3 Antibody - BSA Free (NBP1-77024) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-A20/TNFAIP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of human A20/TNFAIP3 . The immunogen is located within amino acids 340 - 390 of A20/TNFAIP3 Antibody. Amino Acid Squence: VQHEYKKWQENSDQARR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFAIP3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  A20/TNFAIP3 Protein (NBP1-77024PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for A20/TNFAIP3 Antibody - BSA Free

• A20
• A20TNFA1P2
• EC 3.4.19.12
• EC 6.3.2.-
• MGC104522
• MGC138687
• OTU domain-containing protein 7C
• OTUD7C
• OTUD7CMGC138688
• Putative DNA-binding protein A20
• TNF alpha-induced protein 3
• TNFA1P2
• TNFAIP3
• tumor necrosis factor alpha-induced protein 3
• tumor necrosis factor inducible protein A20
• tumor necrosis factor, alpha-induced protein 3
• Zinc finger protein A20

Background

A20, TNFalpha-induced protein 3, TNFAIP3 (theoretical molecular weight 95 kDa) is an important regulator of pro-inflammatory signaling pathways such as NF-kB activation and tumor necrosis factor (TNF)-mediated programmed cell death (1, 2). In macrophages, A20/TNFAIP3 controls the release of IL-1 beta/IL-18 by regulating NLRP3 inflammasome activity and CXCL9/CXCL10 production via STAT1 signaling. By regulating the expression of inflammatory molecules such as IL-6 and anti-apoptotic proteins in dendritic cells, A20/TNFAIP3 maintains T-cell and B-cell homeostasis.

A20/TNFAIP3 is a highly conserved protein sharing >90% amino acid sequence identity across various mammalian species and is highly expressed in T-cell and B-cells. Coding and non-coding single nucleotide polymorphisms (SNPs) of A20/TNFAIP3 have been associated with multiple autoinflammatory and autoimmune diseases including type 1 diabetes, rheumatoid arthritis, Crohn's disease, and systemic lupus erythematosus (SLE) (3). The two domains of A20/TNFAIP3 cooperate to regulate NF-kB signaling. Its N-terminal ovarian tumor (OTU) domain contains a catalytic cysteine (C103) which functions as a K63 deubiquitinase, whereas the 7 zinc fingers that make up the C-terminal domain mediate K48 polyubiquitination. To regulate NF-kB signaling, A20/TNFAIP3 removes K63-polyubiquitin chains from receptor-interacting protein 1 (RIP1) and NF-kB essential modulator (NEMO), thus preventing interactions with downstream partners. A20/TNFAIP3 also contributes to the degradation of RIP1 and Ubc13 through the addition of K48 polyubiquitin chains (4).

References

1.Dixit VM1, Green S, Sarma V, Holzman LB, Wolf FW, O'Rourke K, Ward PA, Prochownik EV, Marks RM. (1990) Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin. J Biol Chem. 265(5):2973-8. PMID: 2406243

2.Verstrepen L, Verhelst K, van Loo G, Carpentier I, Ley SC, Beyaert R. (2010) Expression, biological activities and mechanisms of action of A20 (TNFAIP3). Biochem Pharmacol. 80(12):2009-20. PMID: 20599425

3.Mele A, Cervantes JR, Chien V, Friedman D, Ferran C. (2014) Single nucleotide polymorphisms at the TNFAIP3/A20 locus and susceptibility/resistance to inflammatory and autoimmune diseases. Adv Exp Med Biol. 809:163-83. PMID: 25302371

4.Das T, Chen Z, Hendriks RW, Kool M. (2018) A20/Tumor Necrosis Factor alpha-Induced Protein 3 in Immune Cells Controls Development of Autoinflammation and Autoimmunity: Lessons from Mouse Models. Front Immunol. 9:104. PMID: 29515565

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A20/TNFAIP3 Antibody (NBP1-77024)(1)

Publication using NBP1-77024

• Bao Z, Fan L, Zhao L et al. Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis Front. Mol. Neurosci. 2019-09-19 [PMID: 31607859] (WB, Rat)

FAQs for A20/TNFAIP3 Antibody (NBP1-77024). (Showing 1 - 1 of 1 FAQ).

1. Following cleavage of A20/TNFAIP3, two full length fragments are released, one with 37 kDa and other one was approx. at 52 kDa. There are mentioning of 75Kda fragment and 37 KDa fragment in scientific papers but not 52 KDa. What is the significance of that specific band?
   • There is data available that explains 52 KDa band in A20/TNFAIP3. Using this antibody in Western Blot will result multiple bands. In LPS treatment, 52 KDa putative cleavage band was observed; however, the same band was not found in untreated THP1 cells.

Bioinformatics

• Gene Symbol: TNFAIP3
• Uniprot:
  • Q60769(Mouse)