TRAF-1 Antibody - BSA Free

Western Blot: TRAF-1 Antibody [NB100-56170] - Analysis of TRAF-1 in normal human tissues using this antibody. TRAF-1 is observed at 50-50 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-1 degradation fragments.

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-56170] - Formalin-fixed, paraffin-embedded human tissue sections stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A, A1: Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A, Hodgkin'sDisease, the Reed-Sternberg cells are positive for TRAF-1 expression. B, lymph node, dendritic-like cells are positive for TRAF-1 expression. A1 and B1 are higher magnifications from A and B, respectively.

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-56170] - Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.

• Reactivity: Hu, Bv, Ca
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRAF-1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids between 1 and 50 of human TRAF1 was used as immunogen, GenBank no. NP_005649.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  TRAF-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for TRAF-1 Antibody - BSA Free

• EBI6
• EBI6MGC:10353
• Epstein-Bar virus-induced protein 6
• Epstein-Barr virus-induced protein 6
• TNF receptor-associated factor 1
• TRAF1
• TRAF-1

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. This antibody recognizes TRAF1; human TRAF1 is a 416 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-1 Antibody (NB100-56170)(1)

Publication using NB100-56170

• Zapata JM, Krajewska M, Krajewski S et al. TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies. J Immunol. 2000-11-01 [PMID: 11046039] (IHC-P, WB, Human)
  
  Details:
  WB: Figs 1, 2, 4, 5; IHC-paraffin: Fig 3.

Bioinformatics

• Gene Symbol: TRAF1
• Entrez:
  • 7185(Human)