A20/TNFAIP3 Antibodies
A20, TNF Alpha Induced Protein 3 (TNFAIP3), a zinc finger protein containing ubiquitin and deubiquitinase activities, is an important regulator of proinflammatory signaling including the NF-kappa B pathway and TNF-mediated apoptosis. We offer antibodies to detect TNFAIP3 that have been cited in 40+ publications. Browse our extensive catalog for polyclonal, monoclonal, a recombinant rabbit monoclonal antibody, and conjugated anti-A20 antibodies. Validated research applications for TNFAIP3 antibodies include Chromatin Immunoprecipitation (ChIP), CyTOF-ready, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IP, Flow Cytometry, Simple Western, and Western Blot. Our A20 antibodies can be used in a variety of model species including Human, Mouse, Rat, and Rhesus Macaque. Choose the A20/TNFAIP3 antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each A20/TNFAIP3 antibody is fully covered by our Guarantee+.
Alternate Names for A20/TNFAIP3 Antibodies
anti-A20/TNFAIP3 antibody, anti-TNFAIP3 antibody, anti-A20TNFA1P2 antibody, anti-EC 3.4.19.12 antibody, anti-EC 6.3.2.- antibody, anti-MGC104522 antibody, anti-MGC138687 antibody, anti-OTU domain-containing protein 7C antibody, anti-OTUD7CMGC138688 antibody, anti-Putative DNA-binding protein A20 antibody, anti-TNF alpha-induced protein 3 antibody, anti-tumor necrosis factor alpha-induced protein 3 antibody, anti-tumor necrosis factor inducible protein A20 antibody, anti-tumor necrosis factor, alpha-induced protein 3 antibody, anti-Zinc finger protein A20 antibody
17 Results for "a20-tnfaip3" in Primary Antibodies
HAF018). A specific band was detected for A20/TNFAIP3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI-H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for A20/TNFAIP3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="A20/TNFAIP3 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
![Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Western-Blot-NBP1-77533-img0005.jpg "Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.")
![Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Flow-Cytometry-NBP1-77533-img0013.jpg)
![Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Simple-Western-NBP1-77533-img0008.jpg)
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Immunohistochemistry-Paraffin-NBP1-77533-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-A20/TNFAIP3 (59A426) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Immunocytochemistry-Immunofluorescence-NBP1-77533-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-A20/TNFAIP3 Antibody [59A426] NBP1-77533 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Immunocytochemistry-Immunofluorescence-NBP1-77533-img0018.jpg)
![Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on RH-30 cells with A20/TNFAIP3 Antibody (59A426) NBP1-77533PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-59A426-Flow-Cytometry-NBP1-77533-img0017.jpg)
![Western Blot: A20/TNFAIP3 Antibody [NBP1-77024] - Analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-Western-Blot-NBP1-77024-img0004.jpg "Western Blot: A20/TNFAIP3 Antibody [NBP1-77024] - Analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody [NBP1-77024] - Human lung tissue with TNFAIP3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-77024-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] - Immunofluorescence of A20/TNFAIP3 in Human Lung cells with A20/TNFAIP3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77024_rabbit-polyclonal-a20-tnfaip3-antibody-271120242092072.jpg)
![Western Blot: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Western blot analysis of A20/TNFAIP3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Western-Blot-NBP2-67676-img0007.jpg "Western Blot: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Western blot analysis of A20/TNFAIP3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0004.jpg)
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunohistochemistry-Paraffin-NBP2-67676-img0005.jpg)
![Flow Cytometry: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Flow-Cytometry-NBP2-67676-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody (SN07-31) [NBP2-67676] - Staining TNFAIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-SN07-31-Immunocytochemistry-Immunofluorescence-NBP2-67676-img0003.jpg)
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # 
VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # 
VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ![Western Blot: A20/TNFAIP3 Antibody [NBP1-77351] - SK-N-SH cell lysate with TNFAIP3 antibody at 1 ug/mL](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-Western-Blot-NBP1-77351-img0001.jpg "Western Blot: A20/TNFAIP3 Antibody [NBP1-77351] - SK-N-SH cell lysate with TNFAIP3 antibody at 1 ug/mL")
![Immunohistochemistry: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351] - Immunohistochemistry of A20/TNFAIP3 in human lung tissue with A20/TNFAIP3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77351_rabbit-polyclonal-a20-tnfaip3-antibody-2711202419451235.jpg)
![Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351] - Immunofluorescence of A20/TNFAIP3 in Human Lung cells with A20/TNFAIP3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77351_rabbit-polyclonal-a20-tnfaip3-antibody-2711202419463436.jpg)
![Western Blot: A20/TNFAIP3 Antibody (0K8B0) [NBP3-15681] - Western blot analysis of extracts of various cell lines, using A20/TNFAIP3 antibody (NBP3-15681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-0K8B0-Western-Blot-NBP3-15681-img0002.jpg "Western Blot: A20/TNFAIP3 Antibody (0K8B0) [NBP3-15681] - Western blot analysis of extracts of various cell lines, using A20/TNFAIP3 antibody (NBP3-15681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (0K8B0) [NBP3-15681] - Immunohistochemistry of paraffin-embedded human colon carcinoma using A20/TNFAIP3 Rabbit mAb (NBP3-15681) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-0K8B0-Immunohistochemistry-Paraffin-NBP3-15681-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody [NBP2-68857] - Staining of human cell line REH shows localization to vesicles.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68857-img0001.jpg "Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody [NBP2-68857] - Staining of human cell line REH shows localization to vesicles.")
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (TNFAIP3/2813) [NBP3-07730] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with A20/TNFAIP3 Mouse Monoclonal Antibody (A20/TNFAIP3/2813).](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-TNFAIP3-2813-Immunohistochemistry-Paraffin-NBP3-07730-img0002.jpg "Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (TNFAIP3/2813) [NBP3-07730] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with A20/TNFAIP3 Mouse Monoclonal Antibody (A20/TNFAIP3/2813).")
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (TNFAIP3/2813) [NBP3-07730] - Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with A20/TNFAIP3 Mouse Monoclonal Antibody (A20/TNFAIP3/2813).](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-TNFAIP3-2813-Immunohistochemistry-Paraffin-NBP3-07730-img0001.jpg)
![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody [NBP1-89337] - Staining of human lymph node shows cytoplasmic positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/A20-TNFAIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-89337-img0004.jpg "Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody [NBP1-89337] - Staining of human lymph node shows cytoplasmic positivity in germinal center and non-germinal center cells.")
