Endothelial-Cell-Markers reagents: Novus Biologicals

Sort By:

Products (346)

Full Site

page of 29

346 Results for "Endothelial-Cell-Markers"

List

Grid

NB100-2284

CD31/PECAM-1 Antibody

(5 Reviews)

(151 Publications)

0.1 ml

NB100-2284

Staining of CD31/PECAM-1 in Rat Liver sample. IHC-P image submitted by a verified customer review.

Human lung adenocarcinoma using. Antibody at 1:100 with citrate epitope retrieval at pH 6.0.

Analysis in rat muscle blood vessels using. IHC-P image submitted by a verifed customer review.

Matrix-HA supplemented with strontium was implanted subcutaneously in mice, where immunochemistry analysis and histology occurred of the newly formed tissues. (C) Within each matrix: Matrix-HA, Matrix-8Sr-HA and Matrix-50Sr-HA, were indicated by immunostaining of CD31 for the newly formed tissues. (D) Number of vessels within each tissue was quantified using NDP view software. Unit is square mm. Immunostaining analysis of slides occurred for 2 samples per condition and 3 sections were analyzed per sample and per group of matrix. Citation: Ehret C, Aid-Launais R, Sagardoy T, Siadous R, Bareille R, Rey S, et al. (2017) Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation. PLoS ONE 12(9): e0184663. //doi.org/10.1371/journal.pone.0184663

Imaging of HUVEC monolayer on glass substrate. Fixation with 4% PFA. Blocking with Goat Serum at 1:100. Incubation 2 hours at room temperature. ICC/IF image submitted by a verified customer review.

Analysis of canine kidney tissue using CD31/PECAM-1 antibody. Antibody was used at a 1:50 concentration in Casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9 for two hours at 75C. Image from verified customer review.

Analysis in pig SC cells using CD31/PECAM-1 Antibody (red). Blue color showing nucleus labeling. ICC/IF image submitted by a verified customer review.

CD31-PECAM-1-Antibody-Immunohistochemistry-NB100-2284-img0021.jpg

CD31 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verfied customer review.

Immunohistochemistry evaluation of CD31 expression. (a) Representative images of CD31 staining of vessels in the three groups of implanted matrices (scale bar = 100 µm). (b) Quantification of vessel density inside the implanted matrices at 5 weeks post implantation (n = 6; Average ± SD). NS and **denote Non Significant and p < 0.01, respectively.

Immunohistochemistry: Rabbit Polyclonal CD31/PECAM-1 Antibody [NB100-2284] - Staining of CD31 in human lung tissue. Nuclei in blue and CD31 (vessel) in green. Mouse anti-CD31 (1:100, overnight incubation at +4°C), followed by donkey anti-mouse 488 (1:400, 3h at RT). Image from a verified customer review.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - Antibodies selected for characterization of stromal cells & vasculature in FFPE specimens are species-specific. (A, B) Labeling of a patient colon tumor (A) & Colo205 human cell line xenograft (B) with anti-human mitochondrial antibody (single arrow- tumor cells; double arrows- stroma). (C, D) Labeling of a patient pancreatic tumor (C) & Colo205 human cell line xenograft (D) with anti-human CD34. (E, F) Labeling of a patient lung tumor (E) & Colo205 xenograft (F) with anti-human CD31. (G, H) Labeling of a patient lung tumor (G) & Colo205 xenograft (H) with anti-mouse CD34. (bars = 50 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - At the time of second passage, vessels in successfully growing xenografts of four different tumor types surveyed were of murine origin: A-C Colon; D-F Lung; G-I Pancreatic; J-L Renal Cell Carcinoma. For each tumor type, a representative section of an original patient specimen labeled with anti-huCD31 is shown (A, D, G, J). For each tumor, sections of the first passage xenografted tumor when it was resected are also shown; while no huCD31(+) vessels were identified in the xenografts (B, E, H, K), msCD34(+) vessels were abundant (C, F, I, L). (bars = 50 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - Vascularization of an engrafted patient colon tumor. A patient colon tumor was implanted in a cohort of mice & vessel development was analyzed over 8 weeks. Vessels in the original patient specimen labeled strongly for huCD31 (A) & not for msCD34 (D). Representative sections showing loss of huCD31(+) vessels (B- 4 weeks, C- 7 weeks) & presence of msCD34(+) vessels (E- 4 weeks, F- 7 weeks) are shown. The graph (G) summarizes this process; huCD31(+) vessels were rapidly lost, & by one week, mouse vessels were the predominate vessels present in the colon tumors (no data for msCD34 at 2 weeks; bars = 100 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: CD31/PECAM-1 Antibody [NB100-2284] - KCl pulse decreased membrane, but increased nuclear, detection of NHE1 in b.End3 endothelial cells.(A) Representative immunofluorescence images of bEnd.3 cells at two time-points after KCl or aCSF pulse. (B) Representative immunoblots of NHE1, alpha -tubulin, PECAM, & lamin B in cytosol, membrane, & nuclear fractions of bEnd.3 cells harvested at 5 min after KCl or aCSF pulse. (C = cytosol, M = membrane, N = nuclear) Values represent the mean ratio of NHE1 detection ± SEM (n = 11–12). (C) Representative immunoblots indicating NHE1 & alpha -tubulin as a loading control in whole cell lysate of bEnd.3 cells harvested at 5 min after KCl or aCSF pulse. Values represent the % of aCSF-treated relative expression ± SEM (n = 6). (D) Representative immunoblots of NHE1 & alpha -tubulin as a loading control in whole lysate of microvessels harvested at 90 min after cortical injection of KCl or aCSF. Values represent the % of naive relative expression ± SEM (n = 6). # denotes significantly different vs naïve (p<0.01), as assessed by one-way ANOVA (E) Intracellular pH during aCSF or KCl pulse. All data represent mean ± SEM (n = 45). (F) Extracellular pH during aCSF or KCl pulse. All data represent mean ± SEM (n = 6) in triplicate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32469979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NB100-2284] - Impact of T‐AuNPs on endothelial cells in vivo. Immunofluorescent staining for endothelial cells using an anti‐CD 31 antibody revealed an intact endothelial cell monolayer in the uninjured control carotid artery (N = 3). As expected post balloon angioplasty, there is no endothelial cell monolayer of the injured left carotid arteries in the injury alone, injury + T‐AuNP, & injury + S‐AuNP treatment conditions. Green indicates autofluorescence of the elastic lamina. Magenta indicates endothelial cells. Images obtained using 25× magnification with exposure time of 400 msec. TUNEL staining for apoptosis revealed no evidence of endothelial cell apoptosis in the uninjured control carotid arteries, with a prominent endothelial cell monolayer (white arrowhead). Mild apoptosis was noted in the media of balloon‐injured left carotid arteries, as expected. Black arrows indicate apoptotic cells. Representative images obtained using 40× magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28242820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - The growth of a patient mesothelioma xenograft was supported by development of a murine vascular network. A patient mesothelioma was implanted in a cohort of mice & monitored for tumor growth. Once tumors began to actively grow, representative tumors were resected at weekly intervals & analyzed for vessel content. Staining for huCD31 was prominent in the original patient specimen (A), much reduced at 4 weeks (B) & negligible at 9 weeks (C). In contrast, patient specimens were not stained for msCD34 (D), whereas at 4 weeks large numbers of vessels stained for msCD34 (E) & by 9 weeks, msCD34 labeled vessels were predominant (F). The graph (G) summarizes the loss of detectable human vessels & acquisition of murine vessels over a 9 week period. (bars = 100 μ). Image collected & cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NB100-2284] - TGF‐ beta 1 released by MSCs contributes to induced myofibroblast differentiation & granulation tissue formationA–C2.5 × 105 of TGF‐ beta 1 siRNA or control siRNA‐transfected AT‐MSCs were intradermally injected around each of CD18−/− murine wound. PBS mock injection served as negative control. Wound tissue was harvested at day 2, 5, & 7 post‐wounding for quantification of human TGF‐ beta 1 mRNA (A) at day 2 by qPCR, total TGF‐ beta 1 (B), & active TGF‐ beta 1 (C) protein at day 5 by ELISA. Data are expressed as mean ± SEM, n = 3 wounds per group, **P < 0.01, by one‐way ANOVA with Tukey's test.D–GExpression of alpha ‐SMA (D & E) & CD31 (F & G) at days 5 & 7 by immunostaining on tissue sections. The dashed lines indicate the border of the wound bed & epidermis or eschar. e, epidermis; es, eschar; wb, wound bed. Scale bars: 100 μm. Quantification data are expressed as mean ± SEM, n = 3 wounds per group, *P < 0.05, ***P < 0.001, by one‐way ANOVA with Tukey's test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Rabbit Polyclonal CD31/PECAM-1 Antibody [NB100-2284] - CD31 positive vessels in a canine splenic hemangiosarcoma.Tissue slides were loaded into automated research stainer, dewaxed and pretreated with EDTA-based epitope retrieval ER2 solution for 20 mins at 100°C. After a peroxide incubation for 5 min, the rabbit antibody against CD31 (NB100-2284) (1:100) was incubated for 60 mins at room temperature followed by Bond Polymer (anti-rabbit HRP) incubation for 8 min at room temperature. Mixed DAB reagent (Polymer Refine Detection Kit) was incubated for 10 mins, and Hematoxylin (Refine Detection Kit) counterstaining for 10 mins. After staining, sample slides were washed in water, dehydrated using ethanol gradient (70%, 90%, 100%), washed three times, and mounted in mounting medium. Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NB400-144

CD36 Antibody - BSA Free

(9 Reviews)

(138 Publications)

0.1 ml

NB400-144

Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of CD36 in human adipocytes.

Western Blot: CD36 Antibody [NB400-144] - Mouse primary muscle cell lysate. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD36 Antibody [NB400-144] - Bone marrrow derived mouse macrophages stained with CD36 antibody. ICC/IF image submitted by a verified customer review.

Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.

ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular senescence, while elevated gamma H2AX level indicates accumulated DNA damage. (b) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in mouse embryonic fibroblasts (MEFs). (c) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in brain tissues isolated from 3-, 10-, and 18-month-old male mice. (d) SA-beta -Gal staining in HSFs treated with sh-ATM or scramble shRNA. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Immunoblots showing increased gamma H2AX and unaffected LC3I/II in HSFs treated with sh-ATM or scramble shRNA. (g) Immunoblots showing protein levels of pS1981 ATM, gamma H2AX, and cleaved caspase-3 in HSFs treated with 10 μM of CQ for indicated time. (h) SA-beta -Gal staining in HSFs expressing either scramble or ATM shRNA treated with 1 μM CQ or DMSO (12 hr). Scale bar, 100 µm. (i) Quantification of SA-beta -Gal-positive staining of (h) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001; ‘N.S.’ indicates no significant difference. (j) HSFs at passage 20 were continuously cultured with 1 μM CQ or DMSO, and cell number was calculated at each passage. Data represent means ± SEM. ***p<0.01. (k) Immunoblots showing protein levels of gamma H2AX, p62, and LC3 in MEFs treated with 1 μM CQ or DMSO. Note that CQ had little effect on the expression levels of p62 and LC3. (l) MEFs at passage one were continuously cultured in 20% O2 with 1 μM CQ or DMSO, and cell number was determined at each passage. Data represent means ± SEM. ***p<0.01.10.7554/eLife.34836.006Figure 1—source data 1.Statistical analysis for SA-beta -Gal positive staining.10.7554/eLife.34836.007Figure 1—source data 2.Statistical analysis for EdU positive staining.Sta

Western Blot: Rabbit Polyclonal CD36 Antibody [NB400-144] - The western blot was conducted on avian (passerine) skeletal muscle tissue homogenate. 6ug of total protein was loaded and GAPDH was probed to ensure total protein loads were similar across samples. Rabbit polyclonal CD36 antibody (NB400-144) was used at 1:5000 dilution (diluted in 5% milk). CD36 and GAPDH were probed sequentially - not multiplexed. CD36 produced a band above 40kDa and GAPDH at 36kDa. Image from a verified customer review.

$Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-144] - Timp4-deficiency results in defective lipid digestion & absorption. Fecal triglyceride (TG) content (Ai) in chow- or HFD-fed WT & Timp4−/− mice. Fecal lipid analysis by thin layer chromatography in chow-fed (Aii) & HFD-fed (iii) WT & Timp4−/− mice. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed & HFD-fed WT & Timp4−/− mice. Western blot (C) & mRNA (D) levels for CD36 in enterocyte fraction of chow-fed & HFD-fed WT & Timp4−/− mice (collected from the proximal small intestine). (E) Triglyceride levels in enterocytes collected from the proximal small intestine. Averaged data are presented as mean ± S.E.M & analyzed by ANOVA. * indicates significance (p < 0.05). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: CD36 Antibody - BSA Free [NB400-144] - Timp4-deficiency results in defective lipid digestion & absorption. Fecal triglyceride (TG) content (Ai) in chow- or HFD-fed WT & Timp4−/− mice. Fecal lipid analysis by thin layer chromatography in chow-fed (Aii) & HFD-fed (iii) WT & Timp4−/− mice. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed & HFD-fed WT & Timp4−/− mice. Western blot (C) & mRNA (D) levels for CD36 in enterocyte fraction of chow-fed & HFD-fed WT & Timp4−/− mice (collected from the proximal small intestine). (E) Triglyceride levels in enterocytes collected from the proximal small intestine. Averaged data are presented as mean ± S.E.M & analyzed by ANOVA. * indicates significance (p < 0.05). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: CD36 Antibody - BSA Free [NB400-144] - MiR-29a inhibits the expression of fatty acid translocase CD36 by targeting 3’ untranslated region (UTR). (A) qPCR analysis of Cd36 in live tissue. (B) Representative immunoblotting bands & densitometric results of CD36 in liver tissue. (C) qPCR analysis of cd36 expression of HepG2 cells in vitro after 48h transfection of scramble sequence or miR-29a-mimic. Data obtained from six independent experiments. (D) Upper panel, sequence information, & mutual pairing status of CD36-3’UTR, mmu-miR29a, & CD36-3’UTR Mut. Note that red characters represent mismatching sites. HepG2 was first transfected with CD36-3’UTR or CD36-3’UTR mutant luciferase reporter construct then treated with control medium (ctrl), miRNA-scramble, or miR-29a mimic, & finally lysed to detect the luciferase signal. Data are expressed as mean ± SE. * p < 0.05, ** p < 0.01, & *** p < 0.001 between the indicated groups. WT, wild type mice. HFD, high-fat diet. miR-29a, mice harboring overexpression of miR-29a. mmu-miR29a, mouse-origin miR-29a. ctrl, control. Mut, mutant. UTR, untranslated region. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31652636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD36 Antibody - BSA Free [NB400-144] - Integrin beta 3 modulates SMC transdifferentiation. a–c Mice were fed a HFD for 6 or 16 weeks as indicated, & then transverse aortic root sections were stained. In a, b sections from ApoE(−/−), SMMHC-CreERT2, ROSA26R(mTmG/+) mice were stained for SMA, GFP (fate marker), nuclei (DAPI), & either integrin beta 3 (a) or CD36 (b). Dashed yellow lines separate cap from core (a, b) & core from media (b). n = 5. In c sections from ApoE(−/−) mice that were also wild type or null for Itgb3 were stained for SMMHC, CD68, & nuclei (DAPI). n = 3. Boxed regions (a, c) are shown as close-ups on right; in c CD68+SMMHC+ cells in the media (arrowheads) & plaque (arrows) of the Itgb3 null atherosclerotic aorta are indicated. Med, tunica media; Lu, lumen; Pl, plaque. Scale bars, 25 μm. d–h Aortic SMCs were isolated from ApoE(−/−) mice & then subjected to siRNA-mediated knockdown with si-Itgb3 vs. scrambled (Scr; d–f) or with si-Itgb3, si-Tlr4 vs. si-Itgb3 (g, h). Levels of indicated transcripts from qRT-PCR are relative to Gapdh & normalized to either Scr in d, f or to si-Itgb3 in g, h. For d, g, n = 4–5 in duplicate. In e silenced SMCs were cultured with DiI-conjugated ox-LDL for 10 h & stained with DAPI; n = 5. In f, h silenced SMCs were exposed to soluble cholesterol:methyl-beta -cyclodextrin complexes for 3 days, & then mRNA levels were assessed; n = 4–7 in duplicate. *, **, ***, ^, **** vs. control (Scr in d, f & si-Itgb3 in g, h), p < 0.05, p < 0.01, p < 0.005, p < 0.001, & p < 0.0005, respectively. NS, not significant. Student’s t-test was used, & error bars represent standard deviations. i Schematic of the effect of beta 3 reduction via TLR4 & CD36 in SMCs on transdifferentiation Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04447-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CD36 Antibody - BSA Free [NB400-144] - The increase in CD36 levels caused by lipids in Sirt3-deficient hepatocytes is mediated by Nrf2. VLDLR mRNA abundance (a) & protein levels of VLDLR & NQO1, an Nrf-2-target gene, (b) were assessed in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) & exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. c, fatty acid uptake in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) & exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h was measured by the uptake of BODIPY-C16. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. mRNA abundance (d) & protein levels of VLDLR (e) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA & incubated in the presence or absence 0.3 mM palmitate (Pal) for 24 h. Protein levels of CD36 (f), NQO1 (g) & PPAR gamma (h) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA & incubated in the presence or absence 0.3 mM palmitate (Pal) or the Nrf2 inhibitor ML385 (10 μM) for 24 h. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. CT siRNA cells incubated with palmitate & ML385 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32912335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Pm, Rb

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB400-144AF350
Alexa Fluor 405	NB400-144AF405
Alexa Fluor 488	NB400-144AF488
Alexa Fluor 532	NB400-144AF532
Alexa Fluor 594	NB400-144AF594
Alexa Fluor 647	NB400-144AF647
Alexa Fluor 700	NB400-144AF700
Alexa Fluor 750	NB400-144AF750
Alexa Fluor Plus 488	NB400-144AFP488
Alexa Fluor Plus 555	NB400-144AFP555
Alexa Fluor Plus 594	NB400-144AFP594
Alexa Fluor Plus 647	NB400-144AFP647
Alexa Fluor Plus 680	NB400-144AFP680
Allophycocyanin	NB400-144APC
Biotin	NB400-144B
CoraFluor 1	NB400-144CL1
DyLight 350	NB400-144UV
DyLight 405	NB400-144V
DyLight 488	NB400-144G
DyLight 550	NB400-144R
DyLight 594	NB400-144DL594
DyLight 650	NB400-144C
DyLight 680	NB400-144FR
DyLight 755	NB400-144IR
FITC	NB400-144F
HRP	NB400-144H
Janelia Fluor 525	NB400-144JF525
Janelia Fluor 549	NB400-144JF549
Janelia Fluor 646	NB400-144JF646
Janelia Fluor 669	NB400-144JF669
PE	NB400-144PE
PerCP	NB400-144PCP
mFluor Violet 450 SE	NB400-144MFV450
mFluor Violet 500 SE	NB400-144MFV500
mFluor Violet 610 SE	NB400-144MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB400-144SS

compare

(select up to 3 products from this page to compare)

NB600-562

CD31/PECAM-1 Antibody (JC/70A) - BSA Free

(4 Reviews)

(54 Publications)

0.1 ml

NB600-562

CD31/PECAM-1 was detected in immersion fixed THP-1 (red, positive) but not THP-1 KO (negative) cells using 8 ug/mL Mouse Anti-Human CD31/PECAM-1 (JC/70A) Monoclonal Antibody (Catalog # NB600-562) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Mouse IgG Secondary Antibody (red, Catalog # NL007)) and and counterstained with DAPI (blue).

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for CD31 mRNA (ACD RNAScope probe, catalog # 487381; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (NB600-562) at 1:25 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).

Rabbit Heart, CD31 Stained Red. Image from verified customer review.

A cell surface stain was performed on HUVEC cells with ( NB600-562, blue) along with a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at RT. (Panel A). A negative control (HeLa cells) was also stained to ensure antibody specificity (Panel B).

CD31-PECAM-1-Antibody-JC-70A-Immunohistochemistry-NB600-562-img0022.jpg

Analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.

Analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.

Comparison of the expression of NMDAR1 on vessels and EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects and (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 and NMDAR1 was evident in two groups (yellow, denoted by white arrows). (CF) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) and two mesenchymal markers: FSP1 (red, in panels C and D) and -SMA (red, in panels E and F); DAPI was used as a nuclear stain (blue); co-expression of CD31 and FSP1 or -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 m). Microvascular Injury in Ketamine-Induced Bladder Dysfunction. <i>PLoS One</i> (2016)

A surface stain was performed on Jurkat cells with the Dylight 550 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562R, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody (JC/70A) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Imaging of Feline Endothelial cells with antibody dilution of 1:25. CD31/PECAM-1 (Red), DAPI (Blue). This image was submitted via customer Review.

FFPE human tonsil stained with CD31/PECAM-1 Antibody (JC/70A).

A cell surface stain was performed on Jurkat cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF488, blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 10 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A cell surface stain was performed on THP-1 cells with the Alexa Fluor 488 conjugate of CD31/PECAM-1 Antibody (JC/70A) NB600-562AF488 (blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

A surface stain was performed on THP-1 cells with the Alexa Fluor 647 conjugate of CD31/PECAM-1 Antibody (JC/70A) (NB600-562AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) - BSA Free [NB600-562] - Immunohistochemial comparison of the expression of NMDAR1 on vessels & EndoMT markers in the urinary bladder.Biopsy specimens of frozen sections from the urinary bladders of (A, C, E) normal control subjects & (B, D, F) KC patients. (A, B) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & NMDAR1 (red); DAPI was used as a nuclear stain (blue); co-expression of CD31 & NMDAR1 was evident in two groups (yellow, denoted by white arrows). (C–F) Double-immunofluorescence staining with antibody to endothelial marker CD31 (green) & two mesenchymal markers: FSP1 (red, in panels C & D) & alpha -SMA (red, in panels E & F); DAPI was used as a nuclear stain (blue); co-expression of CD31 & FSP1 or alpha -SMA was evident in three groups (yellow, denoted by white arrows) (white bars: 50 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27529746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

IF staining of IPAH patient CD31/PECAM-1 lung slice. Image from a verified customer review.

Reactivity:

Hu, Mu, Fe, Rb

Species Glossary

Reactivity: Hu, Mu, Fe, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-562AF350
Alexa Fluor 405	NB600-562AF405
Alexa Fluor 488	NB600-562AF488
Alexa Fluor 532	NB600-562AF532
Alexa Fluor 594	NB600-562AF594
Alexa Fluor 647	NB600-562AF647
Alexa Fluor 700	NB600-562AF700
Alexa Fluor 750	NB600-562AF750
Alexa Fluor Plus 405	NB600-562AFP405
Alexa Fluor Plus 488	NB600-562AFP488
Alexa Fluor Plus 555	NB600-562AFP555
Alexa Fluor Plus 594	NB600-562AFP594
Alexa Fluor Plus 647	NB600-562AFP647
Alexa Fluor Plus 680	NB600-562AFP680
Allophycocyanin	NB600-562APC
Biotin	NB600-562B
CoraFluor 1	NB600-562CL1
DyLight 350	NB600-562UV
DyLight 405	NB600-562V
DyLight 488	NB600-562G
DyLight 550	NB600-562R
DyLight 594	NB600-562DL594
DyLight 650	NB600-562C
DyLight 680	NB600-562FR
DyLight 755	NB600-562IR
FITC	NB600-562F
HRP	NB600-562H
Janelia Fluor 525	NB600-562JF525
Janelia Fluor 549	NB600-562JF549
Janelia Fluor 646	NB600-562JF646
Janelia Fluor 669	NB600-562JF669
PE	NB600-562PE
PerCP	NB600-562PCP
mFluor Violet 450 SE	NB600-562MFV450
mFluor Violet 500 SE	NB600-562MFV500
mFluor Violet 610 SE	NB600-562MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-562SS
Azide and BSA Free	NBP2-80641

compare

(select up to 3 products from this page to compare)

NB100-64796

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

(1 Review)

(61 Publications)

0.25 mg

NB100-64796

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis of CD31 in 4th day cutaneous wound section of diabetic rat using CD31/PECAM-1 Antibody (TLD-3A12). Image from verified customer review.

CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Paraffin-NB100-64796-img0014.jpg

(a) HE staining for wound tissues of different groups of rats (magnification ×40). (b) IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification ×200). n = 6, ∗∗P < 0.01.

Reactivity:

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64796AF350
Alexa Fluor 405	NB100-64796AF405
Alexa Fluor 488	NB100-64796AF488
Alexa Fluor 532	NB100-64796AF532
Alexa Fluor 594	NB100-64796AF594
Alexa Fluor 647	NB100-64796AF647
Alexa Fluor 700	NB100-64796AF700
Alexa Fluor 750	NB100-64796AF750
Allophycocyanin	NB100-64796APC
Biotin	NB100-63701
Biotin	NB100-64796B
CoraFluor 1	NB100-64796CL1
DyLight 350	NB100-64796UV
DyLight 405	NB100-64796V
DyLight 488	NB100-64796G
DyLight 550	NB100-64796R
DyLight 594	NB100-64796DL594
DyLight 650	NB100-64796C
DyLight 680	NB100-64796FR
DyLight 755	NB100-64796IR
FITC	NB100-63703-100ug
FITC	NB100-64796F
HRP	NB100-64796H
Janelia Fluor 525	NB100-64796JF525
Janelia Fluor 549	NB100-64796JF549
Janelia Fluor 646	NB100-64796JF646
Janelia Fluor 669	NB100-64796JF669
PE	NB100-64796PE
PerCP	NB100-64796PCP
mFluor Violet 450 SE	NB100-64796MFV450
mFluor Violet 500 SE	NB100-64796MFV500
mFluor Violet 610 SE	NB100-64796MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64796SS

compare

(select up to 3 products from this page to compare)

NB100-2527

LOX Antibody - BSA Free

(5 Reviews)

(40 Publications)

0.1 ml

NB100-2527

Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - LOX in fibrotic mice lung tissue. Primary antibody at 1:100, incubated overnight at 4C. Image from verified customer review.

<b>Genetic Strategies Validation. </b>LOX-Antibody-Knockdown-Validated-NB100-2527-img0016.jpg

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

<b>Biological Strategies Validation. </b>LOX-Antibody-Immunohistochemistry-NB100-2527-img0014.jpg

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.

Western Blot: LOX Antibody [NB100-2527] - Analysis of LOX in human kidney using NB100-2527.

Immunohistochemistry: LOX Antibody [NB100-2527] - Staining of LOX in mouse stomach.

Western Blot: LOX Antibody [NB100-2527] - Patupilone counteracts hypoxia- but not IR-induced LOX-secretion. (A) Western blot of basal & IR-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (B) Western blot of basal & hypoxia-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (C) LOX gene transcription 16 h after irradiation & hypoxia, determined by RT-PCR, averaged over 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2527AF350
Alexa Fluor 405	NB100-2527AF405
Alexa Fluor 488	NB100-2527AF488
Alexa Fluor 532	NB100-2527AF532
Alexa Fluor 594	NB100-2527AF594
Alexa Fluor 647	NB100-2527AF647
Alexa Fluor 700	NB100-2527AF700
Alexa Fluor 750	NB100-2527AF750
Alexa Fluor Plus 405	NB100-2527AFP405
Alexa Fluor Plus 488	NB100-2527AFP488
Alexa Fluor Plus 555	NB100-2527AFP555
Alexa Fluor Plus 594	NB100-2527AFP594
Alexa Fluor Plus 647	NB100-2527AFP647
Alexa Fluor Plus 680	NB100-2527AFP680
Allophycocyanin	NB100-2527APC
Biotin	NB100-2527B
CoraFluor 1	NB100-2527CL1
DyLight 350	NB100-2527UV
DyLight 405	NB100-2527V
DyLight 488	NB100-2527G
DyLight 550	NB100-2527R
DyLight 594	NB100-2527DL594
DyLight 650	NB100-2527C
DyLight 680	NB100-2527FR
DyLight 755	NB100-2527IR
FITC	NB100-2527F
HRP	NB100-2527H
Janelia Fluor 525	NB100-2527JF525
Janelia Fluor 549	NB100-2527JF549
Janelia Fluor 646	NB100-2527JF646
Janelia Fluor 669	NB100-2527JF669
PE	NB100-2527PE
PerCP	NB100-2527PCP
mFluor Violet 450 SE	NB100-2527MFV450
mFluor Violet 500 SE	NB100-2527MFV500
mFluor Violet 610 SE	NB100-2527MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2527SS

compare

(select up to 3 products from this page to compare)

NB600-1008

LYVE-1 Antibody - Azide and BSA Free

(3 Reviews)

(37 Publications)

0.2 mg

NB600-1008

Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

LYVE-1 Antibody-Immunohistochemistry-NB600-1008-img0057.jpg

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.

LYVE-1-Antibody-Immunohistochemistry-NB600-1008-img0052.jpg

Hypoxia, blood vessel & proliferation staining. IHC staining in the Biopsy 23 & Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 & LYVE1. 15× Magnification. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice.a Reduction of Zeb1 mRNA in the heterozygous Zeb−/+ corneas compared to the homozygous Zeb1+/+ corneas. b Significant NV score & vessel size reduction in the Zeb1−/+ corneas as compared to their Zeb1+/+ siblings. c1 A representative stereoscopic image of the Zeb1−/+ & d1 Zeb1+/+ corneas & c2–d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 & the lymphatic vessel marker LYVE-1. *p ≤ 0.05; **p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32620870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1008SS

compare

(select up to 3 products from this page to compare)

NB600-1475

CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

(2 Reviews)

(31 Publications)

0.1 ml

NB600-1475

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified CD31/PECAM-1 Antibody (MEC13.3), followed by anti-rat IgG FITC

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Paraffin-NB600-1475-img0015.jpg

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via customer Review.

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475DL594 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 594.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475G (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CD31/PECAM-1 (MEC13.3) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC13.3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB600-1475R) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1475AF350
Alexa Fluor 405	NB600-1475AF405
Alexa Fluor 488	NB600-1475AF488
Alexa Fluor 532	NB600-1475AF532
Alexa Fluor 594	NB600-1475AF594
Alexa Fluor 647	NB600-1475AF647
Alexa Fluor 700	NB600-1475AF700
Alexa Fluor 750	NB600-1475AF750
Alexa Fluor Plus 405	NB600-1475AFP405
Alexa Fluor Plus 488	NB600-1475AFP488
Alexa Fluor Plus 555	NB600-1475AFP555
Alexa Fluor Plus 594	NB600-1475AFP594
Alexa Fluor Plus 647	NB600-1475AFP647
Alexa Fluor Plus 680	NB600-1475AFP680
Allophycocyanin	NB600-1475APC
Biotin	NB600-1475B
CoraFluor 1	NB600-1475CL1
DyLight 350	NB600-1475UV
DyLight 405	NB600-1475V
DyLight 488	NB600-1475G
DyLight 550	NB600-1475R
DyLight 594	NB600-1475DL594
DyLight 650	NB600-1475C
DyLight 680	NB600-1475FR
DyLight 755	NB600-1475IR
FITC	NB600-1475F
HRP	NB600-1475H
Janelia Fluor 525	NB600-1475JF525
Janelia Fluor 549	NB600-1475JF549
Janelia Fluor 646	NB600-1475JF646
Janelia Fluor 669	NB600-1475JF669
PE	NB600-1475PE
PerCP	NB600-1475PCP
mFluor Violet 450 SE	NB600-1475MFV450
mFluor Violet 500 SE	NB600-1475MFV500
mFluor Violet 610 SE	NB600-1475MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1475SS
Azide and BSA Free	NBP2-80640

compare

(select up to 3 products from this page to compare)

NB600-1071

CD34 Antibody (MEC 14.7) - BSA Free

(30 Publications)

0.1 ml

NB600-1071

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD34 was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rat Anti-Mouse CD34, Monoclonal Antibody (Catalog #NB600-1071) at 1:500 dilution at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD34 was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rat Anti-Mouse CD34, Monoclonal Antibody (Catalog #NB600-1071) at 1:500 dilution at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.

Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as a negative control.

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Notably, the antibody specifically stained the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the colon.

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the heart tissue.

Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).

Reactivity:

Mu, Hu(-)

Species Glossary

Reactivity: Mu, Hu(-)

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1071AF350
Alexa Fluor 405	NB600-1071AF405
Alexa Fluor 488	NB600-1071AF488
Alexa Fluor 532	NB600-1071AF532
Alexa Fluor 594	NB600-1071AF594
Alexa Fluor 647	NB600-1071AF647
Alexa Fluor 700	NB600-1071AF700
Alexa Fluor 750	NB600-1071AF750
Alexa Fluor Plus 405	NB600-1071AFP405
Alexa Fluor Plus 488	NB600-1071AFP488
Alexa Fluor Plus 555	NB600-1071AFP555
Alexa Fluor Plus 594	NB600-1071AFP594
Alexa Fluor Plus 647	NB600-1071AFP647
Alexa Fluor Plus 680	NB600-1071AFP680
Allophycocyanin	NB600-1071APC
Allophycocyanin/Cy7	NB600-1071APCCY7
Biotin	NB600-1071B
CoraFluor 1	NB600-1071CL1
DyLight 350	NB600-1071UV
DyLight 405	NB600-1071V
DyLight 488	NB600-1071G
DyLight 550	NB600-1071R
DyLight 594	NB600-1071DL594
DyLight 650	NB600-1071C
DyLight 680	NB600-1071FR
DyLight 755	NB600-1071IR
FITC	NB600-1071F
HRP	NB600-1071H
Janelia Fluor 525	NB600-1071JF525
Janelia Fluor 549	NB600-1071JF549
Janelia Fluor 646	NB600-1071JF646
Janelia Fluor 669	NB600-1071JF669
PE	NB600-1071PE
PE/Atto594	NB600-1071PEATT594
PE/Cy5.5	NB600-1071PECY55
PE/Cy7	NB600-1071PECY7
PerCP	NB600-1071PCP
mFluor Violet 450 SE	NB600-1071MFV450
mFluor Violet 500 SE	NB600-1071MFV500
mFluor Violet 610 SE	NB600-1071MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1071SS
Azide and BSA Free	NBP2-80642

compare

(select up to 3 products from this page to compare)

NB110-68095

Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

(3 Reviews)

(27 Publications)

0.1 ml

NB110-68095

<b>Biological Strategies Validation. </b>Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Immunocytochemistry-Immunofluorescence-NB110-68095-img0010.jpg

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a verified customer review.

Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Flow-Cytometry-NB110-68095-img0011.jpg

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.

Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Expression of scavenger receptors & immune lectins in rat LSECs & KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), & log2 (iBAQ+ 1)) for scavenger receptors (SR) & C-type lectins in the KC & LSEC transcriptomes & proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC & LSEC transcriptomes & proteomes. The bar height reflects good correlation between the transcriptome & proteome data for gene products of Clec4g, Clec4m, Stab1, & Stab2 in both cell types. The abundance of gene products of Marco & Cd5l were well correlated between the KC transcriptome & proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs & C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, & double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), & to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green & red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 & Fc gamma RIIb2 (SE-1) specifically labeled LSECs & the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, & SR-B1 was observed in both LSECs & KCs Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of scavenger receptors and immune lectins in rat LSECs and KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), and log2 (iBAQ+ 1)) for scavenger receptors (SR) and C-type lectins in the KC and LSEC transcriptomes and proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC and LSEC transcriptomes and proteomes. The bar height reflects good correlation between the transcriptome and proteome data for gene products of Clec4g, Clec4m, Stab1, and Stab2 in both cell types. The abundance of gene products of Marco and Cd5l were well correlated between the KC transcriptome and proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs and C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, and double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), and to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green and red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 and Fc gamma RIIb2 (SE-1) specifically labeled LSECs and the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, and SR-B1 was observed in both LSECs and KCs Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-68095AF350
Alexa Fluor 405	NB110-68095AF405
Alexa Fluor 488	NB110-68095AF488
Alexa Fluor 532	NB110-68095AF532
Alexa Fluor 594	NB110-68095AF594
Alexa Fluor 647	NB110-68095AF647
Alexa Fluor 700	NB110-68095AF700
Alexa Fluor 750	NB110-68095AF750
Alexa Fluor Plus 405	NB110-68095AFP405
Alexa Fluor Plus 488	NB110-68095AFP488
Alexa Fluor Plus 555	NB110-68095AFP555
Alexa Fluor Plus 594	NB110-68095AFP594
Alexa Fluor Plus 647	NB110-68095AFP647
Alexa Fluor Plus 680	NB110-68095AFP680
Allophycocyanin	NB110-68095APC
Allophycocyanin/Cy7	NB110-68095APCCY7
Biotin	NB110-68095B
CoraFluor 1	NB110-68095CL1
DyLight 350	NB110-68095UV
DyLight 405	NB110-68095V
DyLight 488	NB110-68095G
DyLight 550	NB110-68095R
DyLight 594	NB110-68095DL594
DyLight 650	NB110-68095C
DyLight 680	NB110-68095FR
DyLight 755	NB110-68095IR
FITC	NB110-68095F
HRP	NB110-68095H
Janelia Fluor 525	NB110-68095JF525
Janelia Fluor 549	NB110-68095JF549
Janelia Fluor 646	NB110-68095JF646
Janelia Fluor 669	NB110-68095JF669
PE	NB110-68095PE
PE/Atto594	NB110-68095PEATT594
PE/Cy5.5	NB110-68095PECY55
PE/Cy7	NB110-68095PECY7
PerCP	NB110-68095PCP
mFluor Violet 450 SE	NB110-68095MFV450
mFluor Violet 500 SE	NB110-68095MFV500
mFluor Violet 610 SE	NB110-68095MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-68095SS
Azide and BSA Free	NBP2-80759

compare

(select up to 3 products from this page to compare)

NB100-1642

CD31/PECAM-1 Antibody (MEC 7.46) - BSA Free

(29 Publications)

0.1 ml

NB100-1642

CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0011.jpg

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.

Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0010.jpg

Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

CD31/PECAM-1 (MEC 7.46) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC 7.46) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1642AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry for vessel staining (CD31) and beta -amyloid comparing 13- and 24-month old arcA beta mice. Overviews (scale bars = 200 μm) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 μm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26834622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry for vessel staining (CD31) and beta -amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 μm) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 μm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26834622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1642AF350
Alexa Fluor 405	NB100-1642AF405
Alexa Fluor 488	NB100-1642AF488
Alexa Fluor 532	NB100-1642AF532
Alexa Fluor 594	NB100-1642AF594
Alexa Fluor 647	NB100-1642AF647
Alexa Fluor 700	NB100-1642AF700
Alexa Fluor 750	NB100-1642AF750
Alexa Fluor Plus 405	NB100-1642AFP405
Alexa Fluor Plus 488	NB100-1642AFP488
Alexa Fluor Plus 555	NB100-1642AFP555
Alexa Fluor Plus 594	NB100-1642AFP594
Alexa Fluor Plus 647	NB100-1642AFP647
Alexa Fluor Plus 680	NB100-1642AFP680
Allophycocyanin	NB100-1642APC
Biotin	NB100-1642B
CoraFluor 1	NB100-1642CL1
DyLight 350	NB100-1642UV
DyLight 405	NB100-1642V
DyLight 488	NB100-1642G
DyLight 550	NB100-1642R
DyLight 594	NB100-1642DL594
DyLight 650	NB100-1642C
DyLight 680	NB100-1642FR
DyLight 755	NB100-1642IR
FITC	NB100-1642F
HRP	NB100-1642H
Janelia Fluor 525	NB100-1642JF525
Janelia Fluor 549	NB100-1642JF549
Janelia Fluor 646	NB100-1642JF646
Janelia Fluor 669	NB100-1642JF669
PE	NB100-1642PE
PerCP	NB100-1642PCP
mFluor Violet 450 SE	NB100-1642MFV450
mFluor Violet 500 SE	NB100-1642MFV500
mFluor Violet 610 SE	NB100-1642MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1642SS
Azide and BSA Free	NBP2-80639

compare

(select up to 3 products from this page to compare)

NB100-77668

Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

(29 Publications)

0.5 mg

NB100-77668

Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Mouse embryonic cell lysate (A). Antibody at 2 ug/mL.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77668AF350
Alexa Fluor 405	NB100-77668AF405
Alexa Fluor 488	NB100-77668AF488SS
Alexa Fluor 488	NB100-77668AF488
Alexa Fluor 532	NB100-77668AF532
Alexa Fluor 594	NB100-77668AF594
Alexa Fluor 647	NB100-77668AF647
Alexa Fluor 700	NB100-77668AF700
Alexa Fluor 750	NB100-77668AF750
Alexa Fluor Plus 405	NB100-77668AFP405
Alexa Fluor Plus 488	NB100-77668AFP488
Alexa Fluor Plus 555	NB100-77668AFP555
Alexa Fluor Plus 594	NB100-77668AFP594
Alexa Fluor Plus 647	NB100-77668AFP647
Alexa Fluor Plus 680	NB100-77668AFP680
Allophycocyanin	NB100-77668APC-0.1ml
Allophycocyanin	NB100-77668APC-500ul
Allophycocyanin/Cy7	NB100-77668APCCY7
Biotin	NB100-77668B-100ul
DyLight 350	NB100-77668UV-0.1ml
DyLight 350	NB100-77668UV
DyLight 405	NB100-77668V
DyLight 405	NB100-77668V-0.1ml
DyLight 488	NB100-77668G
DyLight 488	NB100-77668G-0.1ml
DyLight 550	NB100-77668R
DyLight 594	NB100-77668DL594
DyLight 650	NB100-77668C
DyLight 680	NB100-77668FR-0.1ml
DyLight 680	NB100-77668FR
DyLight 755	NB100-77668IR
DyLight 755	NB100-77668IR-0.1ml
FITC	NB100-77668F-500ul
HRP	NB100-77668H
Janelia Fluor 549	NB100-77668JF549
Janelia Fluor 646	NB100-77668JF646
PE	NB100-77668PE-0.1ml
PE	NB100-77668PE
PE/Atto594	NB100-77668PEATT594
PE/Cy5.5	NB100-77668PECY55
PE/Cy7	NB100-77668PECY7
PerCP	NB100-77668PCP
PerCP	NB100-77668PCP-0.1ml
mFluor Violet 450 SE	NB100-77668MFV450
mFluor Violet 500 SE	NB100-77668MFV500
mFluor Violet 610 SE	NB100-77668MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-77668SS
Azide and BSA Free	NBP2-80903

compare

(select up to 3 products from this page to compare)

NBP1-71663

CD31/PECAM-1 Antibody - BSA Free

(2 Reviews)

(17 Publications)

0.1 ml

NBP1-71663

<b>Biological Strategies Validation. </b>CD31-PECAM-1-Antibody-Immunohistochemistry-NBP1-71663-img0012.jpg

CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0013.jpg

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).

Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-71663AF350
Alexa Fluor 405	NBP1-71663AF405
Alexa Fluor 488	NBP1-71663AF488
Alexa Fluor 532	NBP1-71663AF532
Alexa Fluor 594	NBP1-71663AF594
Alexa Fluor 647	NBP1-71663AF647
Alexa Fluor 700	NBP1-71663AF700
Alexa Fluor 750	NBP1-71663AF750
Alexa Fluor Plus 405	NBP1-71663AFP405
Alexa Fluor Plus 488	NBP1-71663AFP488
Alexa Fluor Plus 555	NBP1-71663AFP555
Alexa Fluor Plus 594	NBP1-71663AFP594
Alexa Fluor Plus 647	NBP1-71663AFP647
Alexa Fluor Plus 680	NBP1-71663AFP680
Allophycocyanin	NBP1-71663APC
Biotin	NBP1-71663B
CoraFluor 1	NBP1-71663CL1
DyLight 350	NBP1-71663UV
DyLight 405	NBP1-71663V
DyLight 488	NBP1-71663G
DyLight 550	NBP1-71663R
DyLight 594	NBP1-71663DL594
DyLight 650	NBP1-71663C
DyLight 680	NBP1-71663FR
DyLight 755	NBP1-71663IR
FITC	NBP1-71663F
HRP	NBP1-71663H
Janelia Fluor 525	NBP1-71663JF525
Janelia Fluor 549	NBP1-71663JF549
Janelia Fluor 646	NBP1-71663JF646
Janelia Fluor 669	NBP1-71663JF669
PE	NBP1-71663PE
PerCP	NBP1-71663PCP
mFluor Violet 450 SE	NBP1-71663MFV450
mFluor Violet 500 SE	NBP1-71663MFV500
mFluor Violet 610 SE	NBP1-71663MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-71663SS

compare

(select up to 3 products from this page to compare)

page of 29

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD31/PECAM-1 Antibody

CD36 Antibody - BSA Free

CD31/PECAM-1 Antibody (JC/70A) - BSA Free

CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

LOX Antibody - BSA Free

LYVE-1 Antibody - Azide and BSA Free

CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

CD34 Antibody (MEC 14.7) - BSA Free

Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

CD31/PECAM-1 Antibody (MEC 7.46) - BSA Free

Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

CD31/PECAM-1 Antibody - BSA Free