LYVE-1 Antibody

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Positive staining for Lyve-1 on FFPE Mouse Liver, 20x magnification. Antibody diluted 1:800. Image from verified customer review.

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse kidney stained with LYVE-1 antibody. Image from verified customer review.

Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice. c1 A representative stereoscopic image of the Zeb1-/+ and d1 Zeb1+/+ corneas and c2-d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 and the lymphatic vessel marker LYVE-1. *p = 0.05; **p = 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32620870/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Hypoxia, blood vessel and proliferation staining. IHC staining in the Biopsy 23 and Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 and LYVE1. 15x Magnification. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

LYVE-1 Antibody Summary

• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant mouse soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
• Localization: Plasma membrane
• Marker: Lymphatic Vessel Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LYVE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 3-10 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:800
  • Western Blot 2-5 ug/mL
• Application Notes: Use in IHC free floating reported in scientific literature (PMID: 30514806). Use in ICC/IF reported in secitific publication PMID: 32620870
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in (PMID: 24363089).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for LYVE-1 Antibody

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

LYVE-1 (Lymphatic Vessel Endothelial Receptor 1) is a receptor for the extracellular matrix mucopolysaccharide hyaluronan (HA) and is primarily expressed by lymphatic endothelial cells in addition to the sinusoidal endothelium of the liver and spleen (1-3). HA, also called hyaluronic acid, is a main component of the extracellular matrix and functions largely in cell adhesion, migration, and tissue remodeling (2). HA undergoes a turnover process that involves release from the tissues to the afferent lymph, degradation within the lymph nodes, and removal of fragments by the liver (2,3). LYVE-1, in addition to other lymphatic proteins including VEGFR3, Prox1, and podoplanin, is a common marker for differentiating between the blood and lymphatic systems (2,3). Furthermore, LYVE-1 is closely related to the leukocyte receptor CD44, having ~44% sequence similarity (1-3). Like CD44, the LYVE-1 protein contains an extracellular HA-binding link domain, with N- and C-terminal extensions, located at the end of a glycosylated juxtamembrane domain stalk region, followed by a transmembrane region, and a cytoplasmic tail (1-3). The key features of the HA-binding like molecule are the three disulfide bridges formed by six cysteine residues (1-3). LYVE-1 is synthesized as a protein of 322 amino acids in length with a theoretical molecular weight of 35 kDa, although due to O-glycosylation often appears in SDS-PAGE at a molecular weight ranging from ~60-70 kDa (2,4). The role of HA-binding is further elucidated by the identification of LYVE-1 as a docking receptor for dendritic cells and macrophages, binding their surface HA to control the entry and migration into lymph vessels (1).

LYVE-1 has been an important marker in studies of embryonic and tumor lymphangiogenesis, as many cancers are characterized by early metastasis to the lymph nodes (1-3, 5). One study of five different vascular tumors in infants used immunohistochemical analysis and found positive LYVE-1 expression in infantile hemangioma, tufted angioma, and kaposiform hemangioendothelioma (5). LYVE-1 along with other markers such as GLUT-1, CD31, CD34, Prox-1, and WT-1 can be used to help provide immunohistologic profiles of various tumors and, when used in conjunction with clinical and histopathologic approaches, may offer better overall diagnosis and disease treatment (5).

References

1. Jackson D. G. (2019). Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology : Journal of the International Society for Matrix Biology. https://doi.org/10.1016/j.matbio.2018.02.001

2. Jackson D. G. (2004). Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0811.x

3. Jackson D. G. (2003). The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine. https://doi.org/10.1016/s1050-1738(02)00189-5

4. Unitprot (Q9Y5Y7)

5. Johnson, E. F., Davis, D. M., Tollefson, M. M., Fritchie, K., & Gibson, L. E. (2018). Vascular Tumors in Infants: Case Report and Review of Clinical, Histopathologic, and Immunohistochemical Characteristics of Infantile Hemangioma, Pyogenic Granuloma, Noninvoluting Congenital Hemangioma, Tufted Angioma, and Kaposiform Hemangioendothelioma. The American Journal of Dermatopathology. https://doi.org/10.1097/DAD.0000000000000983

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LYVE-1 Antibody (NB600-1008)(24)

Publications using NB600-1008

• Latif A, Fisher LE, Dundas AA et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing Advanced materials (Deerfield Beach, Fla.) 2022-11-28 [PMID: 36440539] (IHC-P, Human)
• Tran NL, Ferreira LM, Alvarez-Moya B et al. Continuous sensing of IFNalpha by hepatic endothelial cells shapes a vascular antimetastatic barrier eLife 2022-10-25 [PMID: 36281643] (IHC-Fr, Mouse)
• Liu Z, Zhang C, Hao J et al. Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis Experimental and Therapeutic Medicine 2022-08-11 [PMID: 36160894] (IHC-P, Rat)
  
  Details:
  Dilution used for IHC 1:100
• Fumagalli V, Venzin V, Di Lucia P et al. Group 1 ILCs regulate T cell-mediated liver immunopathology by controlling local IL-2 availability Science immunology 2022-02-25 [PMID: 35213210]
• De Simone G, Andreata F, Bleriot C Et al. Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming Immunity 2021-08-26 [PMID: 34469774] (IF/IHC, Mouse)
• Burr AC, Velazquez JV, Ulu A Et al. Lung Inflammatory Response to Environmental Dust Exposure in Mice Suggests a Link to Regional Respiratory Disease Risk Journal of inflammation research 2021-08-21 [PMID: 34456580] (IHC-Fr, Mouse)
• Inverso D, Shi J, Lee KH et al. A spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie-Wnt signaling axis in the liver Developmental cell 2021-05-21 [PMID: 34038707] (IHC-P, Mouse)
• Black LM, Winfree S, Khochare SD et al. Quantitative 3-dimensional imaging and tissue cytometry reveals lymphatic expansion in acute kidney injury Laboratory investigation; a journal of technical methods and pathology 2021-05-20 [PMID: 34017058]
• Talamini L, Picchetti P, Ferreira LM et al. Organosilica Cages Target Hepatic Sinusoidal Endothelial Cells Avoiding Macrophage Filtering ACS nano 2021-05-19 [PMID: 34009950]
• Jin L, Zhang Y, Liang W et al. Zeb1 promotes corneal neovascularization by regulation of vascular endothelial cell proliferation Commun Biol 2020-07-03 [PMID: 32620870] (ICC/IF, Mouse)
• Stolley JM, Johnston TS, Soerens AG et al. Retrograde migration supplies resident memory T cells to lung-draining LN after influenza infection J. Exp. Med. 2020-08-03 [PMID: 32568362] (ICC/IF, Mouse)
• Moon Eh, Kim Yh, Vu Pn Et Al. TMEM100 is a key factor for specification of lymphatic endothelial progenitors Angiogenesis 2020-02-28 [PMID: 32112176] (IHC-Wh, Mouse)
• Benechet A, De Simone G, Lucia P et al. Dynamics and Genomic Landscape of CD8 + T Cells Undergoing Hepatic Priming Nature. 2019-10-01 [PMID: 31582858] (IHC-P)
• Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma Burrack A, Spartz E, Raynor et al. Cell Rep. [PMID: 31433988] (IHC-F, Mouse)
• Drosos I, Pavlaki M, Ortega Carrillo MDP et al. Increased Lymphangiogenesis and Lymphangiogenic Growth Factor Expression in Perivascular Adipose Tissue of Patients with Coronary Artery Disease J Clin Med 2019-07-09 [PMID: 31324038] (IF/IHC, Human)
• Kotagama K, Schorr AL, Steber HS, Mangone M Evidence for novel tubular-bundle structures entangled in the fascia of the inner abdominal wall of a rat Micron 2019-05-08 [PMID: 31121482] (IHC-P, Rat)
• Xu K, Lee JY, Kaneko Y et al. Human stem cells transplanted into the rat stroke brain migrate to spleen via lymphatic and inflammation pathways Haematologica 2018-12-04 [PMID: 30514806] (IHC-Fr-Fl, Human)
• Klar AS, Bottcher-Haberzeth S, Biedermann T et al. Analysis of blood and lymph vascularization patterns in tissue-engineered human dermo-epidermal skin analogs of different pigmentation Pediatr Surg Int 2013-12-22 [PMID: 24363089] (IF/IHC, Rat)
• Biedermann T, Klar AS, Bottcher-Haberzeth S et al. Tissue-engineered dermo-epidermal skin analogs exhibit de novo formation of a near natural neurovascular link 10 weeks after transplantation. Pediatr Surg Int 2014-02-01 [PMID: 24363058] (IHC-Fr, Rat)
• Chaudary N, Pintilie M, Schwock J et al. Characterization of the Tumor-Microenvironment in Patient-Derived Cervix Xenografts (OCICx). Cancers (Basel) 2012-01-01 [PMID: 24213469]
• An S, Tiruthani K, Wang Y et al. Locally Trapping the C-C Chemokine Receptor Type 7 by Gene Delivery Nanoparticle Inhibits Lymphatic Metastasis Prior to Tumor Resection Small 2019-01-28 [PMID: 23071319] (IF/IHC, Mouse)
• Kastenmuller W, Torabi-Parizi P, Subramanian N et al. A Spatially-Organized Multicellular Innate Immune Response in Lymph Nodes Limits Systemic Pathogen Spread Cell Cell 2012-09-14 [PMID: 22980983] (IF/IHC, Mouse)
• Kastenmuller K et al. Protective T cell immunity in mice following protein-TLR7/8 agonist-conjugate immunization requires aggregation, type I IFN, and multiple DC subsets. J Clin Invest 2011-05-01 [PMID: 21540549]
• Duignan IJ, Corcoran E, Pennello A et al. Pleiotropic stromal effects of vascular endothelial growth factor receptor 2 antibody therapy in renal cell carcinoma models. Neoplasia13(1):49-59. 2011-01-01 [PMID: 21245940]

Reviews for LYVE-1 Antibody (NB600-1008) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Amleto Fiocchi IHC-P Mouse 06/07/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse liver
• Species: Mouse
• Lot: Lot 1710R10-1

Comments

• Comments: Antibody working dilution 1:800; Citrate pH 6.0. Detection kit Polymer-HRP.

reviewed by: Amleto Fiocchi IHC Mouse 06/29/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Formalin fixed paraffin embedded mouse liver
• Species: Mouse

Comments

• Comments: WD [1:800], HIER with Citrate Buffer pH 6.0 10mM; secondary ab RTU Polymer-HRP

reviewed by: Verified Customer IHC-P Mouse 03/15/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse kidney
• Species: Mouse

Bioinformatics

• Gene Symbol: LYVE1
• Entrez:
  • 114332(Mouse)
• Uniprot:
  • Q8BHC0()