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LYVE-1 Antibody

Images

 
Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Positive staining for Lyve-1 on FFPE Mouse Liver, 20x magnification. Antibody diluted 1:800. Image from verified customer review.
Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse kidney stained with LYVE-1 antibody. Image from verified customer review.
Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice. c1 A representative stereoscopic image of the Zeb1-/+ and d1 Zeb1+/+ corneas and c2-d2 their whole flat-mount ...read more
Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.
Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Hypoxia, blood vessel and proliferation staining. IHC staining in the Biopsy 23 and Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 and LYVE1. 15x Magnification. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

LYVE-1 Antibody Summary

Immunogen
Produced from sera of rabbits immunized with highly pure recombinant mouse soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
Localization
Plasma membrane
Marker
Lymphatic Vessel Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYVE1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 3-10 ug/mL
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:800
  • Western Blot 2-5 ug/mL
Application Notes
Use in IHC free floating reported in scientific literature (PMID: 30514806). Use in ICC/IF reported in secitific publication PMID: 32620870
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-1008 in the following applications:

Publications
Read Publications using
NB600-1008 in the following applications:

Reactivity Notes

Rat reactivity reported in (PMID: 24363089).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for LYVE-1 Antibody

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • UNQ230/PRO263
  • XLKD1

Background

LYVE-1 (Lymphatic Vessel Endothelial Receptor 1) is a receptor for the extracellular matrix mucopolysaccharide hyaluronan (HA) and is primarily expressed by lymphatic endothelial cells in addition to the sinusoidal endothelium of the liver and spleen (1-3). HA, also called hyaluronic acid, is a main component of the extracellular matrix and functions largely in cell adhesion, migration, and tissue remodeling (2). HA undergoes a turnover process that involves release from the tissues to the afferent lymph, degradation within the lymph nodes, and removal of fragments by the liver (2,3). LYVE-1, in addition to other lymphatic proteins including VEGFR3, Prox1, and podoplanin, is a common marker for differentiating between the blood and lymphatic systems (2,3). Furthermore, LYVE-1 is closely related to the leukocyte receptor CD44, having ~44% sequence similarity (1-3). Like CD44, the LYVE-1 protein contains an extracellular HA-binding link domain, with N- and C-terminal extensions, located at the end of a glycosylated juxtamembrane domain stalk region, followed by a transmembrane region, and a cytoplasmic tail (1-3). The key features of the HA-binding like molecule are the three disulfide bridges formed by six cysteine residues (1-3). LYVE-1 is synthesized as a protein of 322 amino acids in length with a theoretical molecular weight of 35 kDa, although due to O-glycosylation often appears in SDS-PAGE at a molecular weight ranging from ~60-70 kDa (2,4). The role of HA-binding is further elucidated by the identification of LYVE-1 as a docking receptor for dendritic cells and macrophages, binding their surface HA to control the entry and migration into lymph vessels (1).

LYVE-1 has been an important marker in studies of embryonic and tumor lymphangiogenesis, as many cancers are characterized by early metastasis to the lymph nodes (1-3, 5). One study of five different vascular tumors in infants used immunohistochemical analysis and found positive LYVE-1 expression in infantile hemangioma, tufted angioma, and kaposiform hemangioendothelioma (5). LYVE-1 along with other markers such as GLUT-1, CD31, CD34, Prox-1, and WT-1 can be used to help provide immunohistologic profiles of various tumors and, when used in conjunction with clinical and histopathologic approaches, may offer better overall diagnosis and disease treatment (5).

References

1. Jackson D. G. (2019). Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology : Journal of the International Society for Matrix Biology. https://doi.org/10.1016/j.matbio.2018.02.001

2. Jackson D. G. (2004). Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0811.x

3. Jackson D. G. (2003). The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine. https://doi.org/10.1016/s1050-1738(02)00189-5

4. Unitprot (Q9Y5Y7)

5. Johnson, E. F., Davis, D. M., Tollefson, M. M., Fritchie, K., & Gibson, L. E. (2018). Vascular Tumors in Infants: Case Report and Review of Clinical, Histopathologic, and Immunohistochemical Characteristics of Infantile Hemangioma, Pyogenic Granuloma, Noninvoluting Congenital Hemangioma, Tufted Angioma, and Kaposiform Hemangioendothelioma. The American Journal of Dermatopathology. https://doi.org/10.1097/DAD.0000000000000983

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LYVE-1 Antibody (NB600-1008)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IF/IHC, IHC, IHC-F, IHC-Fr, IHC-Fr-Fl, IHC-P, IHC-Wh.


Filter By Application
ICC/IF
(2)
IF/IHC
(5)
IHC
(3)
IHC-F
(1)
IHC-Fr
(4)
IHC-Fr-Fl
(1)
IHC-P
(6)
IHC-Wh
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(13)
Rat
(4)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB600-1008 Applications Species
Banda CH, Shiraishi M, Mitsui K et al. Structural and functional analysis of the newt lymphatic system Scientific reports 2023-04-27 [PMID: 37106059] (IHC-P) IHC-P
Azizoglu D, Perez K, Zheng S et al. Liver size is predetermined in the neonate by adding lobules at the periphery bioRxiv 2023-10-14 (IHC, Mouse) IHC Mouse
Latif A, Fisher LE, Dundas AA et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing Advanced materials (Deerfield Beach, Fla.) 2022-11-28 [PMID: 36440539] (IHC-P, Human) IHC-P Human
Tran NL, Ferreira LM, Alvarez-Moya B et al. Continuous sensing of IFNalpha by hepatic endothelial cells shapes a vascular antimetastatic barrier eLife 2022-10-25 [PMID: 36281643] (IHC-Fr, Mouse) IHC-Fr Mouse
Liu Z, Zhang C, Hao J et al. Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis Experimental and Therapeutic Medicine 2022-08-11 [PMID: 36160894] (IHC-P, Rat)

Details:
Dilution used for IHC 1:100
IHC-P Rat
Fumagalli V, Venzin V, Di Lucia P et al. Group 1 ILCs regulate T cell-mediated liver immunopathology by controlling local IL-2 availability Science immunology 2022-02-25 [PMID: 35213210]
Virenque A Characterizing Autophagy in the Cold Preservation of Small Bowel Grafts Thesis 2023-01-01 [PMID: 34876200] (IHC, Mouse) IHC Mouse
De Simone G, Andreata F, Bleriot C Et al. Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming Immunity 2021-08-26 [PMID: 34469774] (IF/IHC, Mouse) IF/IHC Mouse
Burr AC, Velazquez JV, Ulu A Et al. Lung Inflammatory Response to Environmental Dust Exposure in Mice Suggests a Link to Regional Respiratory Disease Risk Journal of inflammation research 2021-08-21 [PMID: 34456580] (IHC-Fr, Mouse) IHC-Fr Mouse
Inverso D, Shi J, Lee KH et al. A spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie-Wnt signaling axis in the liver Developmental cell 2021-05-21 [PMID: 34038707] (IHC-P, Mouse) IHC-P Mouse
Show All 30 Publications.

Reviews for LYVE-1 Antibody (NB600-1008) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB600-1008:
Filter by Applications
IHC-P
(2)
IHC
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin LYVE-1 NB600-1008
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5
reviewed by:
Verified Customer
IHC-P Mouse 06/07/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse liver
SpeciesMouse
LotLot 1710R10-1

Comments

CommentsAntibody working dilution 1:800; Citrate pH 6.0. Detection kit Polymer-HRP.
Immunohistochemistry LYVE-1 NB600-1008
Enlarge
4
reviewed by:
Verified Customer
IHC Mouse 06/29/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedFormalin fixed paraffin embedded mouse liver
SpeciesMouse

Comments

CommentsWD [1:800], HIER with Citrate Buffer pH 6.0 10mM; secondary ab RTU Polymer-HRP
Immunohistochemistry-Paraffin LYVE-1 NB600-1008
Enlarge
5
reviewed by:
Sarah Bowhay
IHC-P Mouse 03/15/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse kidney
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LYVE-1 Antibody (NB600-1008) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on LYVE-1.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
06/07/2019
Application: IHC-P
Species: Mouse

Verified Customer
06/29/2017
Application: IHC
Species: Mouse

Sarah Bowhay
03/15/2016
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol LYVE1
Entrez
Uniprot