LOX Antibody

Images

 
Biological Strategies: Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned ...read more
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - Expression of Lox and Loxl1-Loxl4 mRNAs in orthotopic rat prostate tumours and in the surrounding normal rat prostate tissue. Representative sections from a ...read more
Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - LOX in fibrotic mice lung tissue. Primary antibody at 1:100, incubated overnight at 4C. Image from verified customer review.
Genetic Strategies: Knockdown Validated: LOX Antibody [NB100-2527] - IR-induced LOX promotes the invasive capacity of naive tumor cells. Western blot of LOX in conditioned media derived from untreated and ...read more
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Biological Strategies: Immunohistochemistry: LOX Antibody [NB100-2527] - IR-enhanced LOX in tumor xenografts. Tumor tissue from A549-derived tumor xenografts treated with 1 x 10 Gy and 2 x 10 Gy (with 12 h ...read more
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.
Western Blot: LOX Antibody [NB100-2527] - Analysis of LOX in human kidney using NB100-2527.
Immunohistochemistry: LOX Antibody [NB100-2527] - Staining of LOX in mouse stomach.
Expression of Lox and Loxl1-Loxl4 mRNAs in orthotopic rat prostate tumours and in the surrounding normal rat prostate tissue. (c) Representative sections from a 10-day orthotopic AT-1 rat prostate tumour (T), ...read more
Evidence that hypoxia induces nuclear entry of FIH. (A) Protein levels of HIF1 alpha , HIF2 alpha , FIH and PHD2 in U2OS cells during hypoxia (1% O2) treatment at the indicated time points. GAPDH was used as a loading ...read more
RNase L inhibits SG but not PB assembly and promotes RLB assembly.A, Western blotting analysis of PKR and RNase L in parental (WT), RNase L–KO (RL-KO), PKR-KO, and RNase L/PKR double knockout (RL/PKR-KO) A549 cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LOX Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human LOX protein (within residues 200-300). [Swiss-Prot P28300].
Localization
Secreted, extracellular space.
Predicted Species
Chicken (92%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Knockdown Validated
  • Simple Western 1:25
  • Western Blot 0.5-4ug/ml
Application Notes
This LOX antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western blot. In Western blot, bands are observed ~58 kDa representing glycosylated Lox and ~32 kDa representing the mature, secreted form of Lox. In ICC/IF nuclear staining was observed in HeLa cells, which is expected for the mature form of LOX according to published literature (PMID 17287363 and 10996848).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
LOX Overexpression Lysate
Control Peptide
LOX Peptide (NB100-2527PEP)
Reviewed Applications
Read 5 Reviews rated 4
using
NB100-2527 in the following applications:

Publications
Read Publications using
NB100-2527 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27169768). Immunogen displays the following percentage of sequence identity for non-tested species: bovine (92%), chicken (92%), xenopus (85%). Porcine reactivity reported in scientific literature (PMID: 30036726).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.2
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LOX Antibody

  • AAT10
  • Lysyl Oxidase/LOX
  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into the extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development. Aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX Antibody (NB100-2527)(36)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, Porcine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(8)
IHC
(3)
IHC-P
(7)
WB
(13)
All Applications
Filter By Species
Human
(14)
Mouse
(6)
Rat
(3)
Feline
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NB100-2527 Applications Species
Cheng W, Lawson C, Liu H et al. Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy Animals 2023-06-26 [PMID: 37443910] (IHC-P, Feline) IHC-P Feline
Sasaki M, Hara T, Wang JX et al. Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitis bioRxiv : the preprint server for biology 2023-03-28 [PMID: 37034590] (WB) WB
Wang Q, Sundar IK, Lucas JH et al. Circadian clock molecule REV-ERB? regulates lung fibrotic progression through collagen stabilization Nature communications 2023-03-09 [PMID: 36894533] (IHC-P, WB, Mouse, Human) IHC-P, WB Mouse, Human
Ning Y, Zhou IY, Roberts JD Jr et al. Molecular MRI quantification of extracellular aldehyde pairs for early detection of liver fibrogenesis and response to treatment Science Translational Medicine 2022-09-21 [PMID: 36130015] (IHC) IHC
Jiang C, Wang M, Yao W et al. Comprehensive Analysis on Prognosis and Immune Infiltration of Lysyl Oxidase Family Members in Pancreatic Adenocarcinoma With Experimental Verification Frontiers in Molecular Biosciences 2022-04-01 [PMID: 35433829] (IHC) IHC
Novotny T, Eckhardt A, Doubkova M et al. The possible role of hypoxia in the affected tissue of relapsed clubfoot Scientific reports 2022-03-15 [PMID: 35292718] (IF/IHC, Human) IF/IHC Human
Yang YL, Tsai MC, Chang YH et al. MIR29A Impedes Metastatic Behaviors in Hepatocellular Carcinoma via Targeting LOX, LOXL2, and VEGFA International Journal of Molecular Sciences 2021-06-01 [PMID: 34206143]
Cho W, Kim MS, Lee KH Ionizing radiation attracts tumor targeting and apoptosis by radiotropic lysyl oxidase traceable nanoparticles Nanomedicine 2019-12-10 [PMID: 31830613] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Xu XH, Jia Y, Zhou X et al. Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-b/collagen pathway in preeclampsia Exp. Mol. Med. 2019-02-21 [PMID: 30804321] (IF/IHC, Human) IF/IHC Human
Vadasz Z, Balbir Gurman A, Meroni P et al. Lysyl oxidase-a possible role in systemic sclerosis-associated pulmonary hypertension: a multicentre study Rheumatology (Oxford) 2019-02-15 [PMID: 30770717] (ICC/IF, Human) ICC/IF Human
Show All 36 Publications.

Reviews for LOX Antibody (NB100-2527) (5) 45

Average Rating: 4
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, cat and Rat.

Reviews using NB100-2527:
Filter by Applications
IHC-P
(2)
IF
(1)
WB
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
cat and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin LOX NB100-2527
Enlarge
4
reviewed by:
Wan-Ching Cheng
IHC-P cat and Rat 08/03/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC-P Sample Tested
Speciescat and Rat
LotH-5
Immunofluorescence LOX NB100-2527
Enlarge
4
reviewed by:
Verified Customer
IF Human 06/07/2018
View

Summary

ApplicationImmunofluorescence
Sample Testedfibroblasts
SpeciesHuman
LotH-3
Immunohistochemistry-Paraffin LOX NB100-2527
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 01/14/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lung
SpeciesMouse
LotG-1
Western Blot LOX NB100-2527
Enlarge
4
reviewed by:
Saray Varona
WB Mouse 03/14/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotG-1
  4
reviewed by:
Verified Customer
WB Human 06/19/2009
View

Summary

ApplicationWestern Blot
Sample TestedRenal cell carcinoma cell line UMRC2, Sample Amount: 100ug
SpeciesHuman
LotA

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX Antibody (NB100-2527). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I was wondering if you have any blocking peptides for NB100-59729, NB100-2527 and NB100-2530?
    • Catalog # NB100-2527PEP is the blocking peptide for catalog # NB100-2527.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional LOX Products

Research Areas for LOX Antibody (NB100-2527)

Find related products by research area.

Blogs on LOX.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

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Recent Reviews

4
5
0
4
5
3
0
2
0
1
0

Wan-Ching Cheng
08/03/2018
Application: IHC-P
Species: cat and Rat

Verified Customer
06/07/2018
Application: IF
Species: Human

Verified Customer
01/14/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol LOX