LOX Antibody

Images

 
Western Blot: LOX Antibody [NB100-2527] - Analysis of LOX in human kidney using NB100-2527.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.
Immunohistochemistry: LOX Antibody [NB100-2527] - Staining of LOX in mouse stomach.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - LOX in fibrotic mice lung tissue. Primary antibody at 1:100, incubated overnight at 4C. Image from verified customer review.
Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band detected for LOX in HeLa cell lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system ...read more
Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.3 mg/ml

LOX Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human LOX protein (within residues 200-300). [Swiss-Prot P28300].
Localization
Secreted, extracellular space.
Predicted Species
Porcine (100%), Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-4ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This LOX antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western blot. In Western blot bands are observed ~58 kDa representing glycosylated Lox and ~32 kDa representing the mature, secreted form of Lox. In ICC/IF nuclear staining was observed in HeLa cells, which is expected for the mature form of LOX according to published literature (PMID 17287363 and 10996848).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Kidney Lysate (NB820-59231)
Kidney Lysate (NB820-59661)
LOX Lysate (NBL1-12640)
LOX Lysate (NBP2-64795)
LOX Lysate (NBP2-65238)
Control Peptide
LOX Peptide (NB100-2527PEP)
Reviewed Applications
Read 5 Reviews rated 4
using
NB100-2527 in the following applications:

Publications
Read Publications using
NB100-2527 in the following applications:

  • IHC
    3 publications
  • 4 publications
  • WB
    8 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27169768). Predicted to react with rat pig based on 100% sequence homology. Immunogen sequence has 92% homology to cow and chicken and 85% homology to Xenopus.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LOX Antibody

  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2076
Species: Hu, Po, Ca, Fe, Gt, GP, Sh
Applications: WB, IHC, IHC-P
NBP1-32550
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-59438
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-65875
Species: Hu
NB110-58748
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF
NBP2-01740
Species: Hu, Rt, Ca, Mk, Pm
Applications: WB, ICC/IF, IHC, IHC-P
H00004016-D01P
Species: Hu
Applications: WB, ICC/IF
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-29941
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-75964
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32954
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56713
Species: Hu
Applications: WB, ICC/IF
NB110-58748
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF
AF1927
Species: Hu
Applications: WB, IP, ICC
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB100-2527
Species: Hu, Mu, Rt, Po, Bv, Ch
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for LOX Antibody (NB100-2527)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(3)
IHC-P
(4)
WB
(8)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Rat
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-2527 Applications Species
Pezzoli D, Di Paolo J, kumra H et al. Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds. Biomaterials. Jul 12 2018 [PMID: 30036726] (IHC-P, Porcine) IHC-P Porcine
Wei MM, Wang SS, Zheng JL et al. Herbal compound Teng-Long-Bu-Zhong-Tang inhibits metastasis in human RKO colon carcinoma Oncol Lett 2017 Dec 01 [PMID: 29344222] (IHC-P, Human) IHC-P Human
De Donato M, Petrillo M, Martinelli E et al. Uncovering the role of nuclear Lysyl oxidase (LOX) in advanced high grade serous ovarian cancer. Gynecol. Oncol. May 8 2017 [PMID: 28495238] (IHC, Human) IHC Human
Dodson RB, Miller TA, Powers KN et al. Intrauterine Growth Restriction Influences Vascular Remodeling and Stiffening in the Weanling Rat More than Sex or Diet. Am. J. Physiol. Heart Circ. Physiol. Nov 23 2016 [PMID: 27881387] (Rat) Rat
Gao AE, Sullivan KE, Black LD. Lysyl oxidase expression in cardiac fibroblasts is regulated by alpha2beta1 integrin interactions with the cellular microenvironment. Biochem. Biophys. Res. Commun. Jun 17 2016 [PMID: 27169768] (WB, IHC, Rat) WB, IHC Rat
Nilsson G, Kannius-Janson M. Forkhead Box F1 promotes breast cancer cell migration by upregulating lysyl oxidase and suppressing Smad2/3 signaling. BMC Cancer 2016 Feb 23 [PMID: 26908052] (WB) WB
Nilsson M, Adamo H, Bergh A, Halin Bergstrom S Inhibition of Lysyl Oxidase and Lysyl Oxidase-Like Enzymes Has Tumour-Promoting and Tumour-Suppressing Roles in Experimental Prostate Cancer. Sci Rep 2016 Jan 25 [PMID: 26804196] (IHC) IHC
Hinderer S, Shena N, Ringuette LJ et al. In vitro elastogenesis: instructing human vascular smooth muscle cells to generate an elastic fiber-containing extracellular matrix scaffold. Biomed Mater 2015 Jun [PMID: 25784676] (Human) Human
Ovet H, Oztay F. The Copper Chelator Tetrathiomolybdate Regressed Bleomycin-Induced Pulmonary Fibrosis in Mice, by Reducing Lysyl Oxidase Expressions. Biol Trace Elem Res. 2014 Oct 28 [PMID: 25349139] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Shen CJ, Sharma A, Vuong DV et al. Ionizing radiation induces tumor cell lysyl oxidase secretion. BMC Cancer 2014 Jul 22 [PMID: 25052686] (WB, IHC-P, Human)

Details:
LOX antibody used for WB in conditioned medium from multiple human tumor cell lines in response to irradiation/hypoxia and treatment with microtubule stabilizing agent Patupilone (Epothilone B, EPO906) and in IHC-P on A549-derived tumor xenografts treated with irradiation. WB images shows the detection of LOX zymogen (50 kD) and active form (32 kD) - Figures 1, 3, 4.
WB, IHC-P Human
Show All 19 Publications.

Reviews for LOX Antibody (NB100-2527) (5) 45

Average Rating: 4
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, cat and Rat.

Reviews using NB100-2527:
Filter by Applications
IHC-P
(2)
IF
(1)
WB
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
cat and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin LOX NB100-2527
Enlarge
reviewed by:
Wan-Ching Cheng
IHC-P cat and Rat 08/03/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC-P Sample Tested
Speciescat and Rat
LotH-5
Immunofluorescence LOX NB100-2527
Enlarge
reviewed by:
Anonymous
IF Human 06/07/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested fibroblasts
SpeciesHuman
LotH-3
Immunohistochemistry-Paraffin LOX NB100-2527
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 01/14/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lung
SpeciesMouse
LotG-1
Western Blot LOX NB100-2527
Enlarge
reviewed by:
Saray Varona
WB Mouse 03/14/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotG-1

Blocking

Blocking Details5% milk powder diluted in TBS-Tween 0.05% one hour 25ºtemperature

Primary Anitbody

Dilution RatioLOX diluited in blocking buffer 1:1000, overnight, 4º temperature

Secondary Antibody

Secondary DescriptionPolyclonal Goat Anti-rabbit Immunoglobulins HRP
Secondary Manufacturer Cat#Dako Code No. P 0448
Secondary Concentration1:2000

Details

Detection NotesSuper Signal WestDura, 1minute
 
reviewed by:
Anonymous
WB Human 06/19/2009
View

Summary

ApplicationWestern Blot
Sample TestedRenal cell carcinoma cell line UMRC2, Sample Amount: 100ug
SpeciesHuman
LotA

Blocking

Blocking DetailsBlocking Buffer: 10% MILK, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/200, Incubation Dilution Buffer: 5% milk, Incubation Time: 5 hours, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-Rabbit

Details

Detection NotesDetection Method: ECL, Exposure Time: 10 seconds, Specific Bands: 68 kDa, 32 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX Antibody (NB100-2527). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I was wondering if you have any blocking peptides for NB100-59729, NB100-2527 and NB100-2530?
    • Catalog # NB100-2527PEP is the blocking peptide for catalog # NB100-2527.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2527AF405
Alexa Fluor 488 Labeled NB100-2527AF488
Alexa Fluor 647 Labeled NB100-2527AF647
Alexa Fluor 700 Labeled NB100-2527AF700
Biotin Labeled NB100-2527B
DyLight 405 Labeled NB100-2527V
DyLight 488 Labeled NB100-2527G
DyLight 550 Labeled NB100-2527R
DyLight 650 Labeled NB100-2527C
FITC Labeled NB100-2527F
HRP Labeled NB100-2527H

Additional Array Products

Bioinformatics Tool for LOX Antibody (NB100-2527)

Discover related pathways, diseases and genes to LOX Antibody (NB100-2527). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LOX Antibody (NB100-2527)

Discover more about diseases related to LOX Antibody (NB100-2527).
 

Pathways for LOX Antibody (NB100-2527)

View related products by pathway.

PTMs for LOX Antibody (NB100-2527)

Learn more about PTMs related to LOX Antibody (NB100-2527).
 

Research Areas for LOX Antibody (NB100-2527)

Find related products by research area.

Blogs on LOX.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
5
3
0
2
0
1
0

Wan-Ching Cheng
08/03/2018
Application: IHC-P
Species: cat and Rat

Anonymous
06/07/2018
Application: IF
Species: Human

Anonymous
01/14/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol LOX