Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC]

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Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC] [NB110-68095F] - Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SE-1
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC] Summary

Immunogen
Rat Hepatic Sinusoidal Endothelial Cells
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen 1-5 ug/ml
  • Immunohistochemistry-Paraffin 1-5 ug/ml
Application Notes
This Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) is useful for Immunohistochemistry and Western blot, where a band can be seen around 45-50 kDa. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections.
Reviewed Applications
Read 2 Reviews rated 4
using
NB110-68095F in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [FITC]

  • Hepatic cells
  • Hepatic Sinusoidal Endothelial Cells
  • HSE
  • SE1
  • Sinusoidal Endothelial Cells

Background

Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepatic Sinusoidal Endothelial Cells Antibody (NB110-68095F) (0)

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Reviews for Hepatic Sinusoidal Endothelial Cells Antibody (NB110-68095F) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.
We have 2 reviews tested in 2 application: Flow, IHC-Fr.

Reviews using NB110-68095F:
Filter by Applications
Flow
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(2)
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Images Ratings Applications Species Date Details
Flow Cytometry Hepatic Sinusoidal Endothelial Cells NB110-68095F
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reviewed by:
Maria Martell
Flow Rat 02/28/2017
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Summary

ApplicationFlow Cytometry
Sample TestedLiver sinusoidal endothelial cells
SpeciesRat
LotA-3-020817-F
Immunohistochemistry-Frozen Hepatic Sinusoidal Endothelial Cells NB110-68095F
Enlarge
reviewed by:
Maria Martell
IHC-Fr Rat 01/05/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen Rat Liver Tissue sections
SpeciesRat
LotA-1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Hepatic Sinusoidal Endothelial Cells Antibody (NB110-68095F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB110-68095APC
Biotin Labeled NB110-68095B
DyLight 350 Labeled NB110-68095UV
DyLight 405 Labeled NB110-68095V
DyLight 488 Labeled NB110-68095G
DyLight 550 Labeled NB110-68095R
DyLight 650 Labeled NB110-68095C
DyLight 680 Labeled NB110-68095FR
DyLight 755 Labeled NB110-68095IR
HRP Labeled NB110-68095H
PE Labeled NB110-68095PE

Additional Hepatic Sinusoidal Endothelial Cells Products

Hepatic Sinusoidal Endothelial Cells NB110-68095F

Research Areas for Hepatic Sinusoidal Endothelial Cells Antibody (NB110-68095F)

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Recent Reviews

4
5
0
4
2
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0
2
0
1
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Maria Martell
02/28/2017
Application: Flow
Species: Rat

Maria Martell
01/05/2017
Application: IHC-Fr
Species: Rat