CD34 Antibody (MEC 14.7) [Biotin]

Images

 
There are currently no images for CD34 Antibody (NB600-1071B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clone
MEC 14.7
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin

CD34 Antibody (MEC 14.7) [Biotin] Summary

Immunogen
murine transformed endothelioma cell line t-end.
Localization
Membrane; Single-pass type I membrane protein.
Marker
Hematopoietic Stem Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD34
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This CD34 (MEC 14.7) antibody is useful for Immunohistochemistry (on both paraffin-embedded and frozen sections), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation and ELISA. Antigen retrieval is required for IHC-Paraffin.
Readout System

Reactivity Notes

Mouse. Rat reactivity reported in scientific literature (PMID: 28352335). This antibody does not detect Human CD34 and is an execellent tool for marking host derived endothelial cells/vasculature in human cancer xenografts on mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD34 Antibody (MEC 14.7) [Biotin]

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 Antibody MEC 14.7 (NB600-1071) is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore, it can be used for isolation and characterization of hematopoietic progenitor cells. In Western blot, the reported molecular mass of CD34 is ranging from 100 to 120 kDa, apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NB100-1642
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, Flow-CS
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-25250
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22377
Species: Hu
Applications: WB, IHC, IHC-P
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD34 Antibody (NB600-1071B) (0)

There are no publications for CD34 Antibody (NB600-1071B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD34 Antibody (NB600-1071B) (0)

There are no reviews for CD34 Antibody (NB600-1071B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD34 Antibody (NB600-1071B). (Showing 1 - 1 of 1 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-1071APC
DyLight 350 Labeled NB600-1071UV
DyLight 405 Labeled NB600-1071V
DyLight 488 Labeled NB600-1071G
DyLight 550 Labeled NB600-1071R
DyLight 650 Labeled NB600-1071C
DyLight 680 Labeled NB600-1071FR
DyLight 755 Labeled NB600-1071IR
FITC Labeled NB600-1071F
HRP Labeled NB600-1071H
PE Labeled NB600-1071PE
PerCP Labeled NB600-1071PCP

Additional CD34 Products

Bioinformatics Tool for CD34 Antibody (NB600-1071B)

Discover related pathways, diseases and genes to CD34 Antibody (NB600-1071B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD34 Antibody (NB600-1071B)

Discover more about diseases related to CD34 Antibody (NB600-1071B).
 

Pathways for CD34 Antibody (NB600-1071B)

View related products by pathway.

PTMs for CD34 Antibody (NB600-1071B)

Learn more about PTMs related to CD34 Antibody (NB600-1071B).
 

Research Areas for CD34 Antibody (NB600-1071B)

Find related products by research area.

Blogs on CD34.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial cells. HSCs can be found in bone ma...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD34 Antibody (MEC 14.7) [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol CD34
Entrez