Panendothelial Cell Antigen Antibody (MECA-32) [DyLight 550]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
MECA-32
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 550

Order Details

Panendothelial Cell Antigen Antibody (MECA-32) [DyLight 550] Summary

Immunogen
Mouse lymph node stromal cells.
Specificity
Pan endothelial cells except for CNS & muscle capillaries.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
Plvap
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Panendothelial Cell Antigen Antibody (MECA-32) [DyLight 550]

  • FELS
  • Fenestrated endothelial-linked structure protein
  • gp68
  • MECA32
  • MECA-32
  • Panendothelial Cell Antigen
  • plasmalemma vesicle associated protein
  • Plasmalemma Vesicle Protein 1
  • PV-1 protein

Background

MECA-32 is a single-pass type II membrane homodimer glycoprotein (50-55 kDa) primarily expressed on most endothelial cells in embryonic and adult mouse tissues, except in the brain, skeletal, and cardiac muscle, where expression is more constrained. In the brain, it is only expressed in the circumventricular organs and the neurohemal tissues. In skeletal and cardiac muscle, MECA-32 is confined in small arterioles and venules. In cases such as cardiac transplantation or inflammation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to the first 16 days of gestation and disappears thereafter. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Panendothelial Cell Antigen Antibody (NB100-77668R) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol Plvap