EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

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Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight ...read more
Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD ...read more
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - An intracellular stain was performed on rat FR cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 ...read more
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD ...read more
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [Uniprot: P24942]
Localization
Membrane and ER
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow (Intracellular) 1:500
  • Flow Cytometry 1:200-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 2 ug/ml
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB100-1869 in the following applications:

Publications
Read Publications using
NB100-1869 in the following applications:

  • 1 publication
  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • SPR
    1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

  • EA6
  • EA6FLJ25094
  • EAAT1
  • EAAT1GLAST-1
  • excitatory amino acid transporter 1
  • GLAST
  • GLAST1
  • GLAST-1
  • GLASTGLAST1
  • SLC1A3
  • Sodium-dependent glutamate/aspartate transporter 1
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Solute carrier family 1 member 3

Background

Excitatory amino acid transporter 1 (SLC1A3), also known as sodium-dependent glutamate/aspartate transporter 1, GLAST-1, solute carrier family 1 member 3, EAAT1, and EA6, transports and regulates L-glutamate, D-aspartate, and L-aspartate. SLC1A3 ends the glutamate postsynaptic actions by removing remaining glutamate from the cleft and by reducing extracellular glutamate. SLC1A3 is a member of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. In addition, SLC1A3 may also be important for the prevention of glutamate excitotoxicity. The SLC1A3 protein is located on the cell membrane. SLC1A3 is prominently expressed in the cerebellum, frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. The SLC1A3 protein is subject to glycosylation and phosphorylation. SLC1A3 has two known isoforms (59.5 and 55 kDa) formed by alternative splicing. Mutations in the SLC1A3 gene have been linked to episodic ataxia and hemiplegia (PMID: 16116111) while other mutations result in a form of SLC1A3 (E219D) which influences Tourette syndrome (PMID: 21233784).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, Flow, ICC/IF, IHC, SPR, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(3)
IHC
(1)
SPR
(1)
WB
(9)
All Applications
Filter By Species
Human
(3)
Mouse
(7)
Rat
(5)
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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-1869 Applications Species
Shabanipour S, Jiao X, Rahimi-Balaei M et al. Upregulation of Neural Cell Adhesion Molecule 1 and Excessive Migration of Purkinje Cells in Cerebellar Cortex Frontiers in Neuroscience Jan 21 2022 [PMID: 35126044] (Flow, Mouse) Flow Mouse
Tallon C, Picciolini S, Yoo SW Et al. Inhibition of neutral sphingomyelinase 2 reduces extracellular vesicle release from neurons, oligodendrocytes, and activated microglial cells following acute brain injury Biochemical pharmacology Oct 19 2021 [PMID: 34678224] (SPR, Mouse) SPR Mouse
Wilcox JM, Consoli DC, Tienda AA Et al. Altered synaptic glutamate homeostasis contributes to cognitive decline in young APP/PSEN1 mice Neurobiology of disease Aug 24 2021 [PMID: 34450329] (WB, Mouse) WB Mouse
Balzano T, Leone P, Ivaylova G Et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines Aug 12 2021 [PMID: 34440206] (WB, Rat) WB Rat
La Sala G, Di Pietro C, Matteoni R et al. Gpr37l1/prosaposin receptor regulates Ptch1 trafficking, Shh production, and cell proliferation in cerebellar primary astrocytes Journal of neuroscience research Dec 17 2020 [PMID: 33350496]
Van Gastel N, Spinelli Jb, Sharda A Et Al. Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance Cell Metab. Sep 1 2020 [PMID: 32763164]
Hosseini S, Michaelsen-Preusse K, Grigoryan G et al. Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS Cell Rep May 19 2020 [PMID: 32433975] (WB, ICC/IF, IHC, Mouse) WB, ICC/IF, IHC Mouse
Weng, Q;Wang, J;Wang, J;He, D;Cheng, Z;Zhang, F;Verma, R;Xu, L;Dong, X;Liao, Y;He, X;Potter, A;Zhang, L;Zhao, C;Xin, M;Zhou, Q;Aronow, BJ;Blackshear, PJ;Rich, JN;He, Q;Zhou, W;Suva, ML;Waclaw, RR;Potter, SS;Yu, G;Lu, QR; Single-Cell Transcriptomics Uncovers Glial Progenitor Diversity and Cell Fate Determinants during Development and Gliomagenesis Cell Stem Cell Mar 23 2019 [PMID: 30982771] (ICC/IF, Human) ICC/IF Human
Cabrera-Pastor A, Arenas YM, Taoro-Gonzalez L et al. Chronic hyperammonemia alters extracellular glutamate, glutamine and GABA and membrane expression of their transporters in rat cerebellum. Modulation by extracellular cGMP Neuropharmacology Jan 11 2019 [PMID: 30641078] (WB, Rat) WB Rat
Di Pietro C, La Sala G, Matteoni R et al. Genetic ablation of Gpr37l1 delays tumor occurrence in Ptch1+/- mouse models of medulloblastoma. Exp. Neurol. Nov 16 2018 [PMID: 30452905] (ICC/IF, Mouse) ICC/IF Mouse
Show All 20 Publications.

Review for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1869:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Human 09/08/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869). (Showing 1 - 2 of 2 FAQs).

  1. Would it be possible to know the protein sequence of 20 residue C-terminal synthetic peptide (Rat) used to generate the SLC1A3 Antibody? I would like to try it in Drosophila brains and would like to know if the epitope is conserved.
    • Unfortunately, the sequence used to generate NB100-1869 is considered proprietary, so I am unable to send it to you.
  2. Do you offer cat# NB100-91662 and NB100-1869AF488 labelled with Cy5 or APC ?
    • NB100-1869 is validated for Flow, both types of IHC (frozen and paraffin), and ICC/IF ... and already has several conjugates available.https://www.novusbio.com/products/eaat1-glast-1-slc1a3-antibody_nb100-1869. We could add this antibody conjugated to APC or Cy5 into our system if interested. NB100-91662 is not validated for Flow but has been validated for IHC (parrafin only IHC-P) ... and already has several conjugates available. We could add both conjugates, but if these are going to be used for Flow it would not be guaranteed.

Secondary Antibodies

 

Isotype Controls

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Verified Customer
09/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC1A3
Entrez
Uniprot