EAAT1/GLAST-1/SLC1A3 Antibody


Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight ...read more
Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were ...read more
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were ...read more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

Order Details

EAAT1/GLAST-1/SLC1A3 Antibody Summary

A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [Uniprot: P24942]
Membrane and ER
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:200-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Flow (Intracellular) 1:500
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
EAAT1/GLAST-1/SLC1A3 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
NB100-1869 in the following applications:

Read Publications using
NB100-1869 in the following applications:

  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody

  • EA6
  • EA6FLJ25094
  • EAAT1
  • excitatory amino acid transporter 1
  • GLAST1
  • GLAST-1
  • SLC1A3
  • Sodium-dependent glutamate/aspartate transporter 1
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Solute carrier family 1 member 3


Excitatory amino acid transporter 1 (SLC1A3), also known as sodium-dependent glutamate/aspartate transporter 1, GLAST-1, solute carrier family 1 member 3, EAAT1, and EA6, transports and regulates L-glutamate, D-aspartate, and L-aspartate. SLC1A3 ends the glutamate postsynaptic actions by removing remaining glutamate from the cleft and by reducing extracellular glutamate. SLC1A3 is a member of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. In addition, SLC1A3 may also be important for the prevention of glutamate excitotoxicity. The SLC1A3 protein is located on the cell membrane. SLC1A3 is prominently expressed in the cerebellum, frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. The SLC1A3 protein is subject to glycosylation and phosphorylation. SLC1A3 has two known isoforms (59.5 and 55 kDa) formed by alternative splicing. Mutations in the SLC1A3 gene have been linked to episodic ataxia and hemiplegia (PMID: 16116111) while other mutations result in a form of SLC1A3 (E219D) which influences Tourette syndrome (PMID: 21233784).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-1869 Applications Species
La Sala G, Di Pietro C, Matteoni R et al. Gpr37l1/prosaposin receptor regulates Ptch1 trafficking, Shh production, and cell proliferation in cerebellar primary astrocytes Journal of neuroscience research Dec 17 2020 [PMID: 33350496]
Hosseini S, Michaelsen-Preusse K, Grigoryan G et al. Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS Cell Rep May 19 2020 [PMID: 32433975] (WB, ICC/IF, IHC, Mouse) WB, ICC/IF, IHC Mouse
Weng, Q;Wang, J;Wang, J;He, D;Cheng, Z;Zhang, F;Verma, R;Xu, L;Dong, X;Liao, Y;He, X;Potter, A;Zhang, L;Zhao, C;Xin, M;Zhou, Q;Aronow, BJ;Blackshear, PJ;Rich, JN;He, Q;Zhou, W;Suva, ML;Waclaw, RR;Potter, SS;Yu, G;Lu, QR; Single-Cell Transcriptomics Uncovers Glial Progenitor Diversity and Cell Fate Determinants during Development and Gliomagenesis Cell Stem Cell Mar 23 2019 [PMID: 30982771] (ICC/IF, Human) ICC/IF Human
Yang X, Ren H, Wood K et al. Depletion of microglia augments the dopaminergic neurotoxicity of MPTP The FASEB Journal 2018 Jan 22 [PMID: 29401614] (Mouse) Mouse
Reyes-Aguirre LI, Lamas M. Oct4 Methylation-Mediated Silencing As an Epigenetic Barrier Preventing Muller Glia Dedifferentiation in a Murine Model of Retinal Injury. Front Neurosci. Nov 29 2016 [PMID: 27895551] (FLOW, Mouse) FLOW Mouse
Pinner AL, Tucholski J, Haroutunian V et al. Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia Schizophr. Res. Feb 11 2016 [PMID: 26876311] (WB, Human) WB Human
Arnold JC, Salvatore MF. Exercise-mediated increase in nigral tyrosine hydroxylase is accompanied by increased nigral GFR-a1 and EAAC1 expression in aging rats. ACS Chem Neurosci. 2015 Nov 24 [PMID: 26599339] (WB, Rat) WB Rat
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (WB, Human) WB Human
Vollbrecht PJ, Simmler LD, Blakely RD, Deutch AY. Dopamine denervation of the prefrontal cortex increases expression of the astrocytic glutamate transporter GLT-1. J. Neurochem. 2/24/2014 [PMID: 24611756] (WB, Rat) WB Rat
Chotibut T, Davis RW, Arnold JC et al. Ceftriaxone increases glutamate uptake and reduces striatal tyrosine hydroxylase loss in 6-OHDA Parkinson's model. Mol Neurobiol. 2013 Dec 3 [PMID: 24297323] (WB, Rat) WB Rat
Show All 15 Publications.

Review for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1869:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 09/08/2015


ApplicationWestern Blot
Sample TestedHuman cancer cell


Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000


Detection NotesRegular Protocol

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869). (Showing 1 - 2 of 2 FAQs).

  1. Would it be possible to know the protein sequence of 20 residue C-terminal synthetic peptide (Rat) used to generate the SLC1A3 Antibody? I would like to try it in Drosophila brains and would like to know if the epitope is conserved.
    • Unfortunately, the sequence used to generate NB100-1869 is considered proprietary, so I am unable to send it to you.
  2. Do you offer cat# NB100-91662 and NB100-1869AF488 labelled with Cy5 or APC ?
    • NB100-1869 is validated for Flow, both types of IHC (frozen and paraffin), and ICC/IF... and already has several conjugates available.https://www.novusbio.com/products/eaat1-glast-1-slc1a3-antibody_nb100-1869. We could add this antibody conjugated to APC or Cy5 into our system if interested. NB100-91662 is not validated for Flow but has been validated for IHC (parrafin only IHC-P)... and already has several conjugates available. We could add both conjugates, but if these are going to be used for Flow it would not be guaranteed.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1869AF350
Alexa Fluor 405 NB100-1869AF405
Alexa Fluor 488 NB100-1869AF488
Alexa Fluor 532 NB100-1869AF532
Alexa Fluor 594 NB100-1869AF594
Alexa Fluor 647 NB100-1869AF647
Alexa Fluor 700 NB100-1869AF700
Alexa Fluor 750 NB100-1869AF750
Allophycocyanin NB100-1869APC
Biotin NB100-1869B
DyLight 350 NB100-1869UV
DyLight 405 NB100-1869V
DyLight 488 NB100-1869G
DyLight 550 NB100-1869R
DyLight 594 NB100-1869DL594
DyLight 650 NB100-1869C
DyLight 680 NB100-1869FR
DyLight 755 NB100-1869IR
FITC NB100-1869F
HRP NB100-1869H
Janelia Fluor 549 NB100-1869JF549
Janelia Fluor 646 NB100-1869JF646
PE NB100-1869PE
PerCP NB100-1869PCP

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Recent Reviews


Application: WB
Species: Human


Gene Symbol SLC1A3