EAAT1/GLAST-1/SLC1A3 Antibody

Images

 
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Western blot analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.
Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at (1:250) in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - IHC-P analysis of SLC1A3 on mouse brain.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on HeLa cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869F (blue) and a matched isotype control (orange). Cells were ...read more
Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - IHC staining of SLC1A3 in rat cerebellum sections
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/ml) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight ...read more
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/ml) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U-937 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869F (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

EAAT1/GLAST-1/SLC1A3 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942]
Localization
Membrane and ER
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:200-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Flow (Intracellular) 1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
EAAT1/GLAST-1/SLC1A3 Lysate (NBP2-64783)
Reviewed Applications
Read 1 Review rated 3
using
NB100-1869 in the following applications:

Publications
Read Publications using
NB100-1869 in the following applications:

  • 1 publication
  • WB
    5 publications

Reactivity Notes

Based upon 100% immunogen sequence similarity, this antibody is predicted to cross-react with Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody

  • EA6
  • EA6FLJ25094
  • EAAT1
  • EAAT1GLAST-1
  • excitatory amino acid transporter 1
  • GLAST
  • GLAST1
  • GLAST-1
  • GLASTGLAST1
  • SLC1A3
  • Sodium-dependent glutamate/aspartate transporter 1
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Solute carrier family 1 member 3

Background

Excitatory amino acid transporter 1 (SLC1A3), also known as sodium-dependent glutamate/aspartate transporter 1, GLAST-1, solute carrier family 1 member 3, EAAT1, and EA6, transports and regulates L-glutamate, D-aspartate, and L-aspartate. SLC1A3 ends the glutamate postsynaptic actions by removing remaining glutamate from the cleft and by reducing extracellular glutamate. SLC1A3 is a member of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. In addition, SLC1A3 may also be important for the prevention of glutamate excitotoxicity. The SLC1A3 protein is located on the cell membrane. SLC1A3 is prominently expressed in the cerebellum, frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. The SLC1A3 protein is subject to glycosylation and phosphorylation. SLC1A3 has two known isoforms (59.5 and 55 kDa) formed by alternative splicing. Mutations in the SLC1A3 gene have been linked to episodic ataxia and hemiplegia (PMID: 16116111) while other mutations result in a form of SLC1A3 (E219D) which influences Tourette syndrome (PMID: 21233784).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1869
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: FLOW, WB.


Filter By Application
FLOW
(1)
WB
(5)
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Human
(2)
Mouse
(2)
Rat
(3)
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Showing Publications 1 - 9 of 9.
Publications using NB100-1869 Applications Species
Yang X, Ren H, Wood K et al. Depletion of microglia augments the dopaminergic neurotoxicity of MPTP The FASEB Journal 2018 Jan 22 [PMID: 29401614] (Mouse) Mouse
Reyes-Aguirre LI, Lamas M. Oct4 Methylation-Mediated Silencing As an Epigenetic Barrier Preventing Muller Glia Dedifferentiation in a Murine Model of Retinal Injury. Front Neurosci. Nov 29 2016 [PMID: 27895551] (FLOW, Mouse) FLOW Mouse
Pinner AL, Tucholski J, Haroutunian V et al. Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia Schizophr. Res. Feb 11 2016 [PMID: 26876311] (WB, Human) WB Human
Arnold JC, Salvatore MF. Exercise-mediated increase in nigral tyrosine hydroxylase is accompanied by increased nigral GFR-a1 and EAAC1 expression in aging rats. ACS Chem Neurosci. 2015 Nov 24 [PMID: 26599339] (WB, Rat) WB Rat
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (WB, Human) WB Human
Vollbrecht PJ, Simmler LD, Blakely RD, Deutch AY. Dopamine denervation of the prefrontal cortex increases expression of the astrocytic glutamate transporter GLT-1. J. Neurochem. 2/24/2014 [PMID: 24611756] (WB, Rat) WB Rat
Chotibut T, Davis RW, Arnold JC et al. Ceftriaxone increases glutamate uptake and reduces striatal tyrosine hydroxylase loss in 6-OHDA Parkinson's model. Mol Neurobiol. 2013 Dec 3 [PMID: 24297323] (WB, Rat) WB Rat
Salvatore MF, Davis RW, Arnold JC, Chotibut T. Transient striatal GLT-1 blockade increases EAAC1 expression, glutamate reuptake, and decreases tyrosine hydroxylase phosphorylation at ser(19). Experimental Neurology. 2012 Jan 17. [PMID: 22285253]
Kobayashi S Millhorn DE. Hypoxia regulates glutamate metabolism membrane transport in rat PC12 cells. J Neurochem 76:1935-48. 2001 [PMID: 11259512]

Review for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1869:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869). (Showing 1 - 2 of 2 FAQs).

  1. Would it be possible to know the protein sequence of 20 residue C-terminal synthetic peptide (Rat) used to generate the SLC1A3 Antibody? I would like to try it in Drosophila brains and would like to know if the epitope is conserved.
    • Unfortunately, the sequence used to generate NB100-1869 is considered proprietary, so I am unable to send it to you.
  2. Do you offer cat# NB100-91662 and NB100-1869AF488 labelled with Cy5 or APC ?
    • NB100-1869 is validated for Flow, both types of IHC (frozen and paraffin), and ICC/IF... and already has several conjugates available.https://www.novusbio.com/products/eaat1-glast-1-slc1a3-antibody_nb100-1869. We could add this antibody conjugated to APC or Cy5 into our system if interested. NB100-91662 is not validated for Flow but has been validated for IHC (parrafin only IHC-P)... and already has several conjugates available. We could add both conjugates, but if these are going to be used for Flow it would not be guaranteed.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB100-1869V
FITC Labeled NB100-1869F
PE Labeled NB100-1869PE

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Diseases for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869)

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Recent Reviews

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Anonymous
09/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC1A3
Entrez
Uniprot