Human Stomach Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Stomach Whole Tissue Lysate (Adult Normal) [NB820-59260] - analysis of Beta Actin in human stomach tissue lysate using anti-Beta Actin antibody (Cat. #NBP1-47423) followed by goat anti-mouse kappa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Stomach Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Stomach

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 5
using
NB820-59260 in the following application:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Stomach Lysate (NB820-59260) (0)

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Review for Stomach Lysate (NB820-59260) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB820-59260:
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WB
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Images Ratings Applications Species Date Details
Western Blot Stomach NB820-59260
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5
reviewed by:
Ming Lu
WB Human 10/22/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Stomach Whole Tissue Lysate (Adult Normal)
SpeciesHuman
LotA310005
CommentsNovusbio is very generous to give this normal human stomach lysate for 1mg, which will last for a long time if you use 20ug each time. The lysate worked well in my study.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratiob-actin (Novus NBP1-47423), 1:1000, 4C overnight in blocking buffer

Secondary Antibody

Secondary Descriptionanti mouse light chain kappa Novus NB7549
Secondary Manufacturer Cat#Novus NB7549
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives strong band at correct MW.

Comments

CommentsNovusbio is very generous to give this normal human stomach lysate for 1mg, which will last for a long time if you use 20ug each time. The lysate worked well in my study.

Product General Protocols

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Video Protocols

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FAQs for Stomach Lysate (NB820-59260) (0)

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Other Available Formats

NB820-59260

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Recent Reviews

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Ming Lu
10/22/2014
Application: WB
Species: Human