Human Stomach Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Stomach
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Lysate Details for Array
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
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Stomach Lysate (NB820-59260) (1)
Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
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Reviews using NB820-59260:
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Ming Lu WB
Application Western Blot Sample Tested Human Stomach Whole Tissue Lysate (Adult Normal) Species Human Lot A310005 Comments Novusbio is very generous to give this normal human stomach lysate for 1mg, which will last for a long time if you use 20ug each time. The lysate worked well in my study. Blocking
Blocking Details 2% cold fish geletin in TBS buffer, room temperature for 1hour Primary Anitbody
Dilution Ratio b-actin (Novus NBP1-47423), 1:1000, 4C overnight in blocking buffer Secondary Antibody
Secondary Description anti mouse light chain kappa Novus NB7549 Secondary Manufacturer Cat# Novus NB7549 Secondary Concentration 1:5000 Details
Detection Notes 1 min exposure gives strong band at correct MW. Comments
Comments Novusbio is very generous to give this normal human stomach lysate for 1mg, which will last for a long time if you use 20ug each time. The lysate worked well in my study.
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