Western Blot: GABPA Antibody [NBP1-32105] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody diluted at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-32105] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal diluted at 1:1000. ...read more
Genetic Strategies: Knockdown Validated: Rabbit Polyclonal GABPA Antibody [NBP1-32105] - Primary rat neonatal ventricular cardiomyocytes lysates with siRNA targeting Gabpa (siGabpa) and control (siCont). After 5 ...read more
Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-32105] - Rat spleen. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: GABPA Antibody [NBP1-32105] - Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 ug of GABPA antibody [N2C2], Internal. Western blot analysis was performed using GABPA ...read more
GABPA Antibody [NBP1-32105] - Mouse lymph node. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Knockdown Validated Validated for Knockdown from a verified customer review.
Western Blot 1:500-1:3000
51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-32105 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for GABPA Antibody
E4TF1ATranscription factor E4TF1-60
GA binding protein transcription factor, alpha subunit 60kDa
GA-binding protein alpha chain
GA-binding protein transcription factor, alpha subunit (60kD)
GABP subunit alpha
human nuclear respiratory factor-2 subunit alpha, 10
nuclear respiratory factor 2 alpha subunit
Nuclear respiratory factor 2 subunit alpha
This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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