EAAT2/GLT1 Antibody - Azide Free

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Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.
Immunocytochemistry/ Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed ...read more
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, In vivo, ISH
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide Free

Order Details

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Catalog# & Formulation Size Price

EAAT2/GLT1 Antibody - Azide Free Summary

Immunogen
A synthetic peptide from extracellular domain of mouse Excitatory amino acid transporter 2 (EAAT2, EAA2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and human.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC1A2
Purity
Whole Antiserum
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • In vivo assay
  • In-situ Hybridization
  • Western Blot 1:300-1:2000
Application Notes
Although not tested this antibody may work in IHC-Frozen. Use in flow was reported in scientific literature (PMID: 23793269). Use in In-vivo and in ICC/IF reported in scientific literature (PMID 25581361). Use in In-situ Hybridization reported in scientific literature (PMID: 26150391).
Publications
Read Publications using
NBP1-20136 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Whole Antiserum
Reconstitution Instructions
Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for EAAT2/GLT1 Antibody - Azide Free

  • EAAT2
  • GLT1
  • member 2
  • SLC1A2
  • solute carrier family 1 (glial high affinity glutamate transporter), member 2
  • Solute carrier family 1 member 2

Background

Mediates dihydrokainate-sensitive glutamate transport; may play a role in presynaptic terminal glutamate transport and synaptic transmission.; FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum, retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, In vivo, ISH

Publications for EAAT2/GLT1 Antibody (NBP1-20136)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: DB, FLOW, Flow, ICC/IF, IHC, IHC-P, ISH, In vivo, WB.


Filter By Application
DB
(1)
FLOW
(1)
Flow
(1)
ICC/IF
(2)
IHC
(1)
IHC-P
(1)
ISH
(1)
In vivo
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Rat
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-20136 Applications Species
Filippini A, Mutti V, Faustini G et al. Extracellular clusterin limits the uptake of alpha-synuclein fibrils by murine and human astrocytes Glia Oct 12 2020 [PMID: 33045109] (WB, Mouse) WB Mouse
Yamashiro LH, Wilson SC, Morrison HM et al. Interferon-independent STING signaling promotes resistance to HSV-1 in vivo Nat Commun Jul 7 2020 [PMID: 32636381] (Mouse) Mouse
Napit PR, Ali MH, Shakya M et al. NLRX1 Enhances Glutamate Uptake and Inhibits Glutamate Release by Astrocytes Cells Apr 30 2019 [PMID: 31052241] (Flow, Mouse) Flow Mouse
Blanco-Suarez E, Liu TF, kopelevich A, Allen NJ. Astrocyte-Secreted Chordin-like 1 Drives Synapse Maturation and Limits Plasticity by Increasing Synaptic GluA2 AMPA Receptors. Neuron. Oct 12 2018 [PMID: 30344043] (IHC, Mouse) IHC Mouse
Zhang M, Ngo J, Pirozzi F et al. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells. Stem Cell Res Ther. Mar 15 2018 [PMID: 29544541] (ICC/IF, Human) ICC/IF Human
Schwarz JM. Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue J Vis Exp. 2015 Jun 13 [PMID: 26065673]
Murphy-Royal C, Dupuis JP, Varela JA et al. Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission Nat. Neurosci. 2015 Feb 01 [PMID: 25581361] (ISH, In vivo, ICC/IF, Rat) ISH, In vivo, ICC/IF Rat
Schwarz JM, Smith SH, Bilbo SD. FACS analysis of neuronal-glial interactions in the nucleus accumbens following morphine administration. Psychopharmacology (Berl) 2013 Jun 22 [PMID: 23793269] (FLOW, Rat) FLOW Rat
Gur G, Topuz R, Kizilay G The Effect of Ceftriaxone in Valproic Acid-Induced Mouse Model of Autism Advanced Pharmaceutical Bulletin Oct 6 2021 (WB, IHC-P, Mouse) WB, IHC-P Mouse
Radulescu AR, Todd GC, Williams CL et al. Estimating the glutamate transporter surface density in distinct sub-cellular compartments of mouse hippocampal astrocytes PLoS computational biology Feb 1 2022 [PMID: 35120128] (DB, Mouse) DB Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EAAT2/GLT1 Antibody (NBP1-20136). (Showing 1 - 1 of 1 FAQs).

  1. I am writing to you to ask for further information on the GLT-1 antibody (NBP1-20136). It says on your website that this is an antibody which binds to an extracellular epitope on GLT-1. Could you please tell me where this is? Also, do you know if this antibody can be used in live cell imaging or has it only been used in fixed samples?
    • In regards to your inquiry, this product maps to amino acids 143-238. This antibody has never been tested on live cell imaging. So far we have only tested its use on Immunohistochemistry-Paraffin embedded tissues and cell lysates. Due to the fact that it is provided with no preservatives there may be a chance it will work on live cells, but since we have not specifically tested that we cannot guarantee it to work. Please see our Innovators Reward Program for details if you would like to try using this product in a live cell experiment.

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Bioinformatics

Gene Symbol SLC1A2
Entrez
Uniprot