xCT Antibody [DyLight 755]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 755

xCT Antibody [DyLight 755] Summary

Immunogen
This xCT Antibody was prepared from a synthetic peptide made to an N-terminal region of the human xCT protein (between residues 1-50) [UniProt Q9UPY5].
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC7A11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21540084).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for xCT Antibody [DyLight 755]

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • SLC7A11
  • solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-318IR). (Showing 1 - 1 of 1 FAQ).

  1. Do you have a slc7a11 antibody that is conjugated, reacts to mouse, works with cell surface staining, does not need permeabilization?
    • SLC7A11 or xCT antibody NB300-318AF594 targets a cytoplasmic region of the protein, so it should work without permeabilization. However, the data does say it was permeabilized with saponin, so we cannot say for certain if it will work without permeabilization.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-318AF350
Alexa Fluor 405 NB300-318AF405
Alexa Fluor 488 NB300-318AF488
Alexa Fluor 532 NB300-318AF532
Alexa Fluor 594 NB300-318AF594
Alexa Fluor 647 NB300-318AF647
Alexa Fluor 700 NB300-318AF700
Alexa Fluor 750 NB300-318AF750
Biotin NB300-318B
DyLight 350 NB300-318UV
DyLight 405 NB300-318V
DyLight 488 NB300-318G
DyLight 550 NB300-318R
DyLight 594 NB300-318DL594
DyLight 650 NB300-318C
DyLight 680 NB300-318FR
DyLight 755 NB300-318IR
FITC NB300-318F
HRP NB300-318H
Janelia Fluor 549 NB300-318JF549
Janelia Fluor 646 NB300-318JF646
PE NB300-318PE

Additional xCT Products

Bioinformatics Tool for xCT Antibody (NB300-318IR)

Discover related pathways, diseases and genes to xCT Antibody (NB300-318IR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for xCT Antibody (NB300-318IR)

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Pathways for xCT Antibody (NB300-318IR)

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PTMs for xCT Antibody (NB300-318IR)

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Research Areas for xCT Antibody (NB300-318IR)

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Bioinformatics

Gene Symbol SLC7A11