ATF4 Antibody

Images

 
Western Blot: ATF4 Antibody [NB100-852] - Ethanol feeding induced ATF4 expression in mouse liver which was suppressed by Lipin1 overexpression in adipose tissue. Image from verified customer review.
Western Blot: ATF4 Antibody [NB100-852] - Lysate of cell line K562 (35 ug protein in RIPA buffer). Primary antibody at 0.3 ug/ml, 1 hr incubation. Detected by chemiluminescence.
Western Blot: ATF4 Antibody [NB100-852] - Raji cell lysate (RIPA buffer, 35 ug total protein). Primary antibody at 1 ug/ml, 1 hr incubation. ATF4 antibody detected using chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ATF4 Antibody Summary

Immunogen
Peptide with sequence C-EEVRKARGKKRVP corresponding to C-Terminus according to NP_877962.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ATF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 37 kDa band observed in lysate of K562 cell lines (calculated MW of 38.6 kDa band according to NP_001666.2 and of NP_877962.1). A customer has reported positive results on mouse embryonic fibroblasts (3T3 cells).
Theoretical MW
38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATF4 Lysate (NBL1-07790)
Reviewed Applications
Read 1 Review rated 5
using
NB100-852 in the following applications:

Publications
Read Publications using
NB100-852 in the following applications:

  • WB
    3 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF4 Antibody

  • activating transcription factor 4 (tax-responsive enhancer element B67)
  • Activating transcription factor 4
  • ATF4
  • cAMP-dependent transcription factor ATF-4
  • cAMP-responsive element-binding protein 2
  • CREB-2DNA-binding protein TAXREB67
  • cyclic AMP-dependent transcription factor ATF-4
  • Cyclic AMP-responsive element-binding protein 2
  • TAXREB67
  • TAXREB67CREB2cAMP response element-binding protein 2
  • Tax-responsive enhancer element-binding protein 67
  • TXREB

Background

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF4 Antibody (NB100-852)(4)

Review for ATF4 Antibody (NB100-852) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-852:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot ATF4 NB100-852
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reviewed by:
Anonymous
WB Mouse 02/06/2018
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Summary

ApplicationWestern Blot
Sample TestedMouse whole liver
SpeciesMouse
Lotc2

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Video Protocols

WB Video Protocol

FAQs for ATF4 Antibody (NB100-852) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for ATF4 Antibody (NB100-852)

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Blogs on ATF4.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/06/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ATF4
Entrez
Uniprot