Xanthine Oxidase Antibody - BSA Free

• Reactivity: Bv
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Xanthine Oxidase Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Xanthine Oxidase [Bovine Buttermilk] (Uniprot: P80457)
• Localization: Cytoplasmic; Peroxisomal
• Specificity: Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XDH
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Xanthine Oxidase Antibody - BSA Free

• EC 1.17.1.4
• EC 1.17.3.2
• EC 1.7.2.2
• xanthene dehydrogenase
• xanthine dehydrogenase
• xanthine dehydrogenase/oxidase
• Xanthine Oxidase
• xanthine oxidoreductase
• XD
• XDH
• XDHA
• XO
• XOR
• XOR)

Background

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Xanthine Oxidase Antibody (NB600-935)(1)

Publication using NB600-935

• Jacobson, A, C Yan et al. Aging enhances pressure-induced arterial superoxide formation. 293: H1344-1350. 2007-01-01 [PMID: 17557915]

Bioinformatics

• Gene Symbol: XDH
• Uniprot:
  • P80457(Bovine)