Xanthine Oxidase Antibody

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Xanthine Oxidase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Xanthine Oxidase [Bovine Buttermilk]. Uniprot ID P80457
Localization
Cytoplasmic; Peroxisomal
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase from bovine buttermilk.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XDH
Purity
Ion exchange chromatography
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:100,000 of the reconstitution concentration is suggested for this product.
Publications
Read Publication using NB600-935.

Reactivity Notes

Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been tested.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 100 ul deionized water.

Alternate Names for Xanthine Oxidase Antibody

  • EC 1.17.1.4
  • EC 1.7.2.2
  • xanthene dehydrogenase
  • xanthine dehydrogenase
  • xanthine dehydrogenase/oxidase
  • xanthine oxidase
  • xanthine oxidoreductase
  • XDHA
  • XO
  • XOR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Xanthine Oxidase Antibody (NB600-935)(1)


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Bioinformatics

Gene Symbol XDH