Xanthine Oxidase Antibody [HRP]

Dot Blot: Xanthine Oxidase Antibody [HRP] [NB100-2025] - Dot Blot of Rabbit Xanthine Oxidase antibody [HRP]. Xanthine Oxidase (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Xanthine Oxidase HRP at 1.0ug/mL for 1hr at RT. Secondary Antibody: none.Blocking Buffer for 30mins at RT. Exposure: 0.1sec.

Dot Blot of Xanthine Oxidase Antibody [HRP]. Xanthine Oxidase (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Xanthine Oxidase HRP at 1.0ug/mL for 1hr at RT. Secondary Antibody: none. Blocking Buffer: BlockOut Buffer

• Reactivity: Bv
• Applications: WB, DB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP
• Concentration: LYOPH

Xanthine Oxidase Antibody [HRP] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]
• Immunogen: Xanthine Oxidase [Bovine Buttermilk] (Uniprot: P80457)
• Localization: Cytoplasmic; Peroxisomal
• Specificity: Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XDH
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Xanthine Oxidase Antibody [HRP]

• EC 1.17.1.4
• EC 1.17.3.2
• EC 1.7.2.2
• xanthene dehydrogenase
• xanthine dehydrogenase
• xanthine dehydrogenase/oxidase
• Xanthine Oxidase
• xanthine oxidoreductase
• XD
• XDH
• XDHA
• XO
• XOR
• XOR)

Background

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XDH
• Uniprot:
  • P80457(Bovine)