Xanthine Oxidase Antibody [Biotin]

Dot Blot: Xanthine Oxidase Antibody [Biotin] [NB100-2009] - Dot Blot results of Rabbit Xanthine Oxidase antibody [Biotin].Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1ug/mL for 1hr at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT.

Dot Blot results of Rabbit Xanthine Oxidase Antibody [Biotin]. Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1ug/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP

• Reactivity: Bv
• Applications: WB, DB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: LYOPH

Xanthine Oxidase Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]
• Immunogen: Xanthine Oxidase [Bovine Buttermilk] (Uniprot: P80457)
• Localization: Cytoplasmic; Peroxisomal
• Specificity: Cross reactivity against Xanthine Oxidase from other sources is unknown.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XDH
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Cross reactivity against Xanthine Oxidase from other sources is unknown.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Xanthine Oxidase Antibody [Biotin]

• EC 1.17.1.4
• EC 1.17.3.2
• EC 1.7.2.2
• xanthene dehydrogenase
• xanthine dehydrogenase
• xanthine dehydrogenase/oxidase
• Xanthine Oxidase
• xanthine oxidoreductase
• XD
• XDH
• XDHA
• XO
• XOR
• XOR)

Background

In normal tissues this enzyme exists predominantly as Xanthine Dehydrogenase which converts hypoxanthine and xanthine to uric acid, a critical plasma antioxidant. During ischemia, Xanthine Dehydrogenase is reversibly converted to the oxygen radical-producing Xanthine Oxidase by oxidation of essential sulfhydryl groups, or irreversibly, through limited proteolytic cleavage of the amino-terminus. Aldehyde Oxidase has a higher affinity for aldehydes than purines, but produces O2- in a manner very similar to Xanthine Oxidase because both oxidases possess nearly identical internal electron transport systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XDH
• Uniprot:
  • P80457(Bovine)