Vinculin Antibody - BSA Free

Western Blot: Vinculin Antibody [NBP2-20859] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-20859] - Vinculin antibody detects Vinculin protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Vinculin stained by Vinculin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Human hepatoma, using Vinculin antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: Vinculin Antibody [NBP2-20859] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000.

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Mouse testis. Vinculin antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Vinculin Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane; Peripheral membrane protein; Cytoplasmic side
• Marker: Focal Adhesion Marker
• Predicted Species: Porcine (98%), Bovine (98%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VCL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Vinculin Antibody - BSA Free

• CMD1W
• CMH15
• metavinculin
• MVCL
• VCL
• Vinculin

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vinculin Antibody (NBP2-20859)(3)

Publications using NBP2-20859

• Sinha BK, Murphy C, Brown SM et Al. Mechanisms of Cell Death Induced by Erastin in Human Ovarian Tumor Cells Int J Mol Sci 2024-08-29 [PMID: 39201357]
• Silver B, Gerrish K, Tokar E. Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoids eLife 2023-06-01 [PMID: 37261896] (Western Blot, Mouse)
• Huda N, Khambu B, Liu G et al. Senescence Connects Autophagy Deficiency to Inflammation and Tumor Progression in the Liver Cellular and molecular gastroenterology and hepatology 2022-04-08 [PMID: 35398596] (WB, Mouse)

FAQs for Vinculin Antibody (NBP2-20859). (Showing 1 - 1 of 1 FAQs).

1. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Bioinformatics

• Gene Symbol: VCL
• Uniprot:
  • P18206()