Myosin Heavy Chain Antibody (MF20) [Unconjugated]

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Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, ICC/IF
Clone
MF20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Myosin Heavy Chain Antibody (MF20) [Unconjugated] Summary

Immunogen
Chicken pectoralis-derived Myosin
Specificity
Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MYH14
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB4470 in the following applications:

Publications
Read Publications using
MAB4470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myosin Heavy Chain Antibody (MF20) [Unconjugated]

  • DFNA4
  • FLJ13881
  • FLJ43092
  • KIAA2034DKFZp667A1311
  • MHC16
  • MYH14 variant protein
  • MYH17
  • MYHC
  • Myosin heavy chain 14
  • Myosin Heavy Chain
  • Myosin heavy chain, non-muscle IIc
  • myosin
  • myosin, heavy chain 14
  • myosin, heavy chain 14, non-muscle
  • myosin, heavy polypeptide 14
  • myosin-14
  • NMHC II-C
  • NMHC-II-C
  • Non-muscle myosin heavy chain IIc
  • nonmuscle myosin heavy chain II-C

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Heavy Chain Antibody (MAB4470)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Bioassay, ICC, ICC Fresh, IHC, Simple Western, WB, Western Blot.


Filter By Application
Bioassay
(1)
ICC
(7)
ICC Fresh
(1)
IHC
(1)
Simple Western
(1)
WB
(9)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB4470 Applications Species
M Wattin, L Gaweda, P Muller, M Baritaud, C Scholtes, C Lozano, K Gieseler, C Kretz-Remy Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients Int J Mol Sci, 2018;19(1):. 2018 [PMID: 29316663] (WB,, Human) WB Human
M Koide, Y Hagiwara, M Tsuchiya, M Kanzaki, H Hatakeyama, Y Tanaka, T Minowa, T Takemura, A Ando, T Sekiguchi, Y Yabe, E Itoi Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration Tohoku J. Exp. Med., 2018;244(1):15-24. 2018 [PMID: 29311489] (ICC,, Human) ICC Human
H Lee, SJ Lee, GU Bae, NI Baek, JH Ryu Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy Int J Mol Sci, 2017;18(12):. 2017 [PMID: 29258243] (ICC,, Mouse) ICC Mouse
G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90 Nat Commun, 2017;8(1):589. 2017 [PMID: 28928431] (WB,, Mouse) WB Mouse
MM Cleary, A Mansoor, T Settelmeye, Y Ijiri, KJ Ladner, MN Svalina, BP Rubin, DC Guttridge, C Keller NF?B signaling in alveolar rhabdomyosarcoma Dis Model Mech, 2017;10(9):1109-1115. 2017 [PMID: 28883017] (WB,, Mouse) WB Mouse
S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28607151] (WB,, Mouse) WB Mouse
DS Han, WS Yang, TW Kao Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation Int J Med Sci, 2017;14(5):434-443. 2017 [PMID: 28539819] (WB,, Mouse) WB Mouse
F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways Cell Rep, 2017;18(10):2320-2330. 2017 [PMID: 28273449] (ICC,, Mouse) ICC Mouse
J Ueki, M Nakamori, M Nakamura, M Nishikawa, Y Yoshida, A Tanaka, A Morizane, M Kamon, T Araki, MP Takahashi, A Watanabe, N Inagaki, H Sakurai Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability Sci Rep, 2017;7(0):42522. 2017 [PMID: 28211918] (ICC,, Human) ICC Human
CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 27956629]
Show All 22 Publications.

Review for Myosin Heavy Chain Antibody (MAB4470) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4470:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Myosin Heavy Chain MAB4470
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reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
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Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate; 3T3-L1 cell lysate
SpeciesHuman
LotCAEI0314061

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio20 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western. Great antibody for human skeletal muscle tissue; For cell lysates, optimal sample concentration at 0.4 ug/uL/

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Myosin Heavy Chain Antibody (MAB4470) (0)

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Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol MYH14