Myosin Heavy Chain Antibody (MF20) [Unconjugated]

Images

 
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room ...read more
Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
MF20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Myosin Heavy Chain Antibody (MF20) [Unconjugated] Summary

Immunogen
Chicken pectoralis-derived Myosin
Specificity
Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MYH14
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 5-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB4470 in the following applications:

Publications
Read Publications using
MAB4470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myosin Heavy Chain Antibody (MF20) [Unconjugated]

  • DFNA4
  • FLJ13881
  • FLJ43092
  • KIAA2034DKFZp667A1311
  • MHC16
  • MYH14 variant protein
  • MYH17
  • MYHC
  • Myosin heavy chain 14
  • Myosin Heavy Chain
  • Myosin heavy chain, non-muscle IIc
  • myosin
  • myosin, heavy chain 14
  • myosin, heavy chain 14, non-muscle
  • myosin, heavy polypeptide 14
  • myosin-14
  • NMHC II-C
  • NMHC-II-C
  • Non-muscle myosin heavy chain IIc
  • nonmuscle myosin heavy chain II-C

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Heavy Chain Antibody (MAB4470)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: Bioassay, ICC, IHC, Simple Western, Western Blot.


Filter By Application
Bioassay
(1)
ICC
(15)
IHC
(2)
Simple Western
(1)
Western Blot
(17)
All Applications
Filter By Species
Human
(14)
Mouse
(21)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using MAB4470 Applications Species
D Abbadi, M Yang, DM Chenette, JJ Andrews, RJ Schneider Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 31113881] (ICC, Mouse) ICC Mouse
D Liu, X Qiao, Z Ge, Y Shang, Y Li, W Wang, M Chen, S Si, SZ Chen IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway Skelet Muscle, 2019;9(1):8. 2019 [PMID: 30922397] (Western Blot, Mouse) Western Blot Mouse
LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30853557] (IHC, Mouse) IHC Mouse
AV Bhat, M Palanicham, VK Rao, L Pignata, HJ Lim, S Suriyamurt, KTE Chang, VK Lee, E Guccione, R Taneja Epigenetic regulation of the PTEN-AKT-RAC1 axis by G9a is critical for tumor growth in alveolar rhabdomyosarcoma Cancer Res., 2019;0(0):. 2019 [PMID: 30833420] (ICC, Human) ICC Human
J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects Cell Rep, 2019;26(9):2494-2508.e7. 2019 [PMID: 30811996]
MC Estañ, E Fernández-, MS Zaki, MI Esteban, S Donkervoor, C Hawkins, JA Caparros-M, D Saade, Y Hu, V Bolduc, KR Chao, J Nevado, A Lamuedra, R Largo, G Herrero-Be, J Regadera, C Hernandez-, EF Tizzano, V Martinez-G, JJ Carvajal, R Zong, DL Nelson, GA Otaify, S Temtamy, M Aglan, M Issa, CG Bönnemann, P Lapunzina, G Yoon, VL Ruiz-Perez Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy Nat Commun, 2019;10(1):797. 2019 [PMID: 30770808] (Western Blot, Mouse) Western Blot Mouse
RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations Stem Cells Int, 2019;2019(0):5690345. 2019 [PMID: 30719048] (ICC, Human) ICC Human
YP Li, TK Sin, JZ Zhu, G Zhang p300 mediates muscle wasting in Lewis lung carcinoma Cancer Res., 2019;0(0):. 2019 [PMID: 30705122] (Western Blot, Mouse) Western Blot Mouse
X Xiang, S Langlois, ME St-Pierre, JF Barré, D Grynspan, B Purgina, KN Cowan Pannexin 1 inhibits rhabdomyosarcoma progression through a mechanism independent of its canonical channel function Oncogenesis, 2018;7(11):89. 2018 [PMID: 30459312] (ICC, Human) ICC Human
T Osaki, SGM Uzel, RD Kamm Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons Sci Adv, 2018;4(10):eaat5847. 2018 [PMID: 30324134] (Western Blot, Human) Western Blot Human
Show All 38 Publications.

Review for Myosin Heavy Chain Antibody (MAB4470) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4470:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Myosin Heavy Chain MAB4470
Enlarge
reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
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Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate; 3T3-L1 cell lysate
SpeciesHuman
LotCAEI0314061

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio20 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western. Great antibody for human skeletal muscle tissue; For cell lysates, optimal sample concentration at 0.4 ug/uL/

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Myosin Heavy Chain Antibody (MAB4470) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin IC4470A
Fluorescein IC4470F

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Recent Reviews

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Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol MYH14