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Zyxin Antibody (2C10-4A7)

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Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.Lane 1: ZYX transfected lysate(61.3 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of monoclonal antibody to ZYX on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Immunoprecipitation: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal ...read more
ELISA: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, KD
Clone
2C10-4A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Zyxin Antibody (2C10-4A7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
Marker
Focal Adhesion Marker
Specificity
ZYX - zyxin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ZYX
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA, RNAi Validation and IF.
Publications
Read Publications using H00007791-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Zyxin Antibody (2C10-4A7)

  • CTCL tumor antigen se14-3
  • CTCL-associated antigen se14-3
  • cutaneous T-cell lymphoma associated antigen se14-3
  • Cutaneous T-cell lymphoma-associated antigen se14-3
  • KIAA1125
  • MGC31836
  • predicted protein of HQ2893
  • PRKCBP1
  • PRO2893
  • protein kinase C-binding protein 1
  • Rack7
  • RACK7protein kinase C binding protein 1
  • zinc finger MYND domain containing protein 8
  • Zinc finger MYND domain-containing protein 8
  • zinc finger, MYND-type containing 8
  • Zyx
  • Zyxin 2
  • Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Zyxin Antibody (H00007791-M01)(7)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZYX
Entrez
OMIM
Uniprot