Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.Lane 1: ZYX transfected lysate(61.3 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of monoclonal antibody to ZYX on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Immunoprecipitation: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal ...read more
Sandwich ELISA: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.
WB, ELISA, ICC/IF, IP, S-ELISA
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
ZYX (AAH08743, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag.MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGP
Focal Adhesion Marker
ZYX - zyxin
Test in a species/application not listed above to receive a full credit towards a future purchase.
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.