Vinculin Antibodies: Novus Biologicals

NB600-1293

Vinculin Antibody (hVIN-1)

(1 Review)

(16 Publications)

0.1 ml

NB600-1293

Immunohistochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Enhanced Validation-By Independent Antibodies:Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Heart sections stained with 15 ug/mL Anti-Vinculin antibody produced in Rabbit (Cat. No. V4139) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 15 ug/mL Monoclonal Anti-Vinculin antibody produced in Mouse, Clone: hVIN1 (Cat. No. V9131) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody.Results:Two Anti-Vinculin antibodies, V4139 (A) and V9131 (B), target different regions of Vinculin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.

A1B decreases TLR4 protein content in microglia chronically treated with LPS. (A) N9 cells seeded at 1 × 105 cells per well on poly-d-lysine-coated glass cover slides in 6-well plates were pre-treated for 4 h with DMSO or with 0.5 uM K-604, then exposed with or without 200 ng/mL LPS for 24 h. Double immunofluorescence staining for TLR4 and for the plasma membrane marker N-cadherin was then performed. (i) Timeline of the experiment. (ii) Representative images demonstrating TLR4 distribution in N9 cells. (iii) Quantification of total TLR4 relative fluorescence intensity per cell, n = 15 cells/treatment group. (iv) Quantification of IM/PM TLR4 fluorescence intensity ratio. A total of 15 cells per group were analyzed. The TLR4 signals overlapping with that of N-cadherin are considered as TLR4 at the PM, while those not overlapping are considered as TLR4 at the IM. (B) N9 microglial cells were seeded at 2 × 105 cells per well onto 6-well plates in RPMI-1640 with 10% serum. N9 cells were treated with DMSO (control group) or 0.5 uM K-604 for 4 h, then treated with or without 200 ng/mL LPS for 24 and 48 h. At different time points, cells were harvested for protein isolation and TLR4 Western blot analyses. n = 3 replicates. Vinculin was used as the protein loading control. (i) Timeline of experiment. (ii) Western blot. (iii) Quantitation of Western blot. * p < 0.05; ** p < 0.01; **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/6/5616), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reduction of directionality and focal adhesion turnover by low eribulin concentrations(A) Effect of eribulin on MTOC directionality during wound healing. Percentage of cells with MTOC facing the wound (bottom). Values are mean +/- SEM (n = 3) **P < 0.01. (B) immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for vinculin (red) and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 μm. Quantitative analysis of the area of vinculin staining (right). Values are mean +/- SEM (≥30 cells per group). **P < 0.01. (C) Immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for Tyr397-phosphorylated FAK (green), actin (red), and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 μm. Quantitative analysis of the area of Tyr397-phosphorylated FAK staining (right). Values are mean +/- SEM (≥30 cells per group). *P < 0.05. Eribulin treatment shrank the Tyr397-phosphorylated FAK staining area in a dose-dependent manner. (D) Western blot of Tyr397-phosphorylated FAK in LM8 cells treated with eribulin (top). Quantitative densitometric analysis of the ratio of Tyr397-phosphorylated FAK to total FAK (bottom). Values are mean +/- SEM (n = 3). *P < 0.05. (E) Immunofluorescence images of LM8 cells treated with 0 nM or 10 nM eribulin and stained for APC (red), alpha -tubulin (green), and nucleus (blue). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30719211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB6896

Vinculin Antibody (728526) [Unconjugated]

(3 Reviews)

(19 Publications)

100 μg

MAB6896

HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Vinculin at approximately 122 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Vinculin Monoclonal Antibody (Catalog # MAB6896). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Cr and Ni exposure increases tau levels and phosphorylation in iNeurons and RA-differentiated SH-SY5Y cells. (a,b) iPSCs carrying the R406W tau mutation and isogenic controls were differentiated into iNeurons and treated with Cr (5 µM) and Ni (800 µM) for 72 hours. The levels of total (a) and phospho-tauSer396/404 (PHF-1) (b) tau were measured by western blot. GAPDH and vinculin were used as loading controls. Images show representative immunoblots comparing tau and phospho-tau levels in control and mutant iNeurons before and after Cr and Ni treatment. Plots represents the average ± SEM of 3 independent experiments. (c,d) RA-differentiated SH-SY5Y cells were treated with Cr (2.5 µM) and Nickel (200 µM) for 24 hours before protein extraction. Images show representative immunoblots comparing the levels total tau (c) and phospho-tauSer396/404 (PHF-1) tau (d) before and after heavy metals treatment. GAPDH and vinculin were used as loading control. Plots represent the average ± SEM of 4 independent experiments. Statistical significance was determined by two‐ANOVA followed by Bonferroni’s test for multiple comparisons or one-way ANOVA using GraphPad Prism 6. p-values comparing untreated and heavy metal treated cells are included in the graphs. Statistical significance was considered when p-value ≤ 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953414), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Cr and Ni exposure induces apoptotic cell death in SH-SY5Y cells. Non-differentiated and RA-differentiated SH-SY5Y cells were treated with Cr (2.5 µM) and Ni (200 µM) for 24 hours. Whole cell lysates were collected in order to analyze by western blot the levels of cleaved caspase-3 protein (a), the ratio of anti-apoptotic protein Bcl2 to pro-apoptotic protein Bax (b) and the cleavage and activation of caspase-9 (c). Vinculin and GAPDH were used as loading controls. (a) Representative immunoblot showing the presence of the 17/19KD fragment of caspase-3 after Cr and Ni exposure. (b) Representative immunoblots showing the levels of Bcl2 and Bax proteins after heavy metal exposure. The activation of the intrinsic/mitochondrial apoptosis pathway was assessed by the decrease of the ratio of Bcl2 to full length Bax and the presence of a cleavage form of Bax protein. The plot represent the average ± SEM of 3 independent experiments. Statistical significance was determined by one-way analysis of variance (anova) followed by Bonferroni’s test for multiple comparisons using GraphPad Prism 6. p-values comparing untreated and heavy metal treated cells are included. Statistical significance was considered when p-value ≤ 0.05. (c) Immunoblot showing the presence of the fragment (43KD) of active caspase-9 after Cr and Ni exposure to confirm the activation of the intrinsic/mitochondrial pathway. All experiments were performed in triplicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953414), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western: Vinculin Antibody (728526) [Unconjugated] [MAB6896] - Western blot analysis for expression levels of mTOR and its downstream factors in the DRG on PID 21. Phosphorylated expressions of mTOR complex 1 and 2 were decreased in the shmTOR group, and its downstream effectors were downregulated as well. Data are expressed as mean +- SEM, * p < 0.05, ** p < 0.01, **** p < 0.0001. One-way ANOVA with Tukey's multiple comparisons test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37958901), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6896U-100UG
Alexa Fluor 405	FAB6896V-100UG
Alexa Fluor 488	FAB6896G-100UG
Alexa Fluor 532	FAB6896X-100UG
Alexa Fluor 594	FAB6896T-100UG
Alexa Fluor 647	FAB6896R-100UG
Alexa Fluor 700	FAB6896N-100UG
Alexa Fluor 750	FAB6896S-100UG
Alexa Fluor Plus 405	FAB6896AFP405-100UG
Alexa Fluor Plus 488	FAB6896AFP488-100UG
Alexa Fluor Plus 555	FAB6896AFP555-100UG
Alexa Fluor Plus 594	FAB6896AFP594-100UG
Alexa Fluor Plus 647	FAB6896AFP647-100UG
Alexa Fluor Plus 680	FAB6896AFP680-100UG
Allophycocyanin	FAB6896A
Biotin	FAB6896B
CoraFluor 1	FAB6896CL1
DyLight 350	FAB6896D
DyLight 405	FAB6896E
DyLight 488	FAB6896K
DyLight 550	FAB6896L
DyLight 594	FAB6896M
DyLight 650	FAB6896W
DyLight 680	FAB6896Y
DyLight 755	FAB6896Z
FITC	FAB6896F
HRP	FAB6896H
Janelia Fluor 525	FAB6896JF525
Janelia Fluor 549	FAB6896I
Janelia Fluor 646	FAB6896J
Janelia Fluor 669	FAB6896JF669
PE	FAB6896P
PerCP	FAB6896C
mFluor Violet 450 SE	FAB6896MFV450
mFluor Violet 500 SE	FAB6896MFV500
mFluor Violet 610 SE	FAB6896MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6896-SP

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NBP2-20859

Vinculin Antibody - Azide Free

(3 Publications)

0.1 ml

NBP2-20859

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Mouse testis. Vinculin antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Effects of erastin (2.5 uM) on various oxidative and ferroptosis-related genes in OVCAR-8 (A) and NCI/ADR-RES cells (B) cells following treatment with erastin for 4 h and 24 h. Protein levels for GPX4, NrF2, and NOX4 following treatment with 2.5 uM for 4 and 24 h in OVCAR-8 and NCI/ADR-RES cells (C). * p < 0.05, ** and *** p values < 0.005 and 0.001, respectively, compared to control ( beta -Actin at 4 h and 24 h, respectively). Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/16/8666), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-87495

Vinculin Antibody - BSA Free

0.1 ml

NBP1-87495

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Stainig of human cerebral cortex shows no positivity in neuronal cells.

Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in human heart tissue.

Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Stainig of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human testis shows strong cytoplasmic postivity in cells in seminiferious ducts.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87495-25ul

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NB120-11193

Vinculin Antibody (VIN-11-5)

(2 Publications)

0.1 ml

NB120-11193

Reactivity:

Hu, Mu, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Bv, Ch

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-41237

Vinculin Antibody - BSA Free

0.1 mg

NBP2-41237

Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Human PC-3 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41237 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41237] - Validation of Vinculin in Rat Small Intestine Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat small intestine tissue labeling Vinculin with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry: Vinculin Antibody - BSA Free [NBP2-41237] - Validation of Vinculin in Rat Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Vinculin antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Reactivity:

Hu, Mu, Rt, Po, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-41237AF350
Alexa Fluor 405	NBP2-41237AF405
Alexa Fluor 488	NBP2-41237AF488
Alexa Fluor 532	NBP2-41237AF532
Alexa Fluor 594	NBP2-41237AF594
Alexa Fluor 647	NBP2-41237AF647
Alexa Fluor 700	NBP2-41237AF700
Alexa Fluor 750	NBP2-41237AF750
Alexa Fluor Plus 405	NBP2-41237AFP405
Alexa Fluor Plus 488	NBP2-41237AFP488
Alexa Fluor Plus 555	NBP2-41237AFP555
Alexa Fluor Plus 594	NBP2-41237AFP594
Alexa Fluor Plus 647	NBP2-41237AFP647
Alexa Fluor Plus 680	NBP2-41237AFP680
Allophycocyanin	NBP2-41237APC
Biotin	NBP2-41237B
CoraFluor 1	NBP2-41237CL1
DyLight 350	NBP2-41237UV
DyLight 405	NBP2-41237V
DyLight 488	NBP2-41237G
DyLight 550	NBP2-41237R
DyLight 594	NBP2-41237DL594
DyLight 650	NBP2-41237C
DyLight 680	NBP2-41237FR
DyLight 755	NBP2-41237IR
FITC	NBP2-41237F
HRP	NBP2-41237H
Janelia Fluor 525	NBP2-41237JF525
Janelia Fluor 549	NBP2-41237JF549
Janelia Fluor 646	NBP2-41237JF646
Janelia Fluor 669	NBP2-41237JF669
PE	NBP2-41237PE
PerCP	NBP2-41237PCP
mFluor Violet 450 SE	NBP2-41237MFV450
mFluor Violet 500 SE	NBP2-41237MFV500
mFluor Violet 610 SE	NBP2-41237MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-41237-0.025mg

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NBP2-55796

Vinculin Antibody - BSA Free

100 ul

NBP2-55796

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-55796-25ul

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AF6896

Vinculin Antibody [Unconjugated]

100 μg

AF6896

HAF016). A specific band was detected for Vinculin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat Vinculin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6896) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Vinculin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat Vinculin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6896) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vinculin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western shows lysates of Exosome Standards (PC‑3) (NBP2-49856) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Vinculin at approximately 119 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat Vinculin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6896). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). vView our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Sheep Anti-Human/Rat Vinculin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6896) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). vView our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6896U-100UG
Alexa Fluor 405	AF6896V-100UG
Alexa Fluor 488	AF6896G-100UG
Alexa Fluor 532	AF6896X-100UG
Alexa Fluor 594	AF6896T-100UG
Alexa Fluor 647	AF6896R-100UG
Alexa Fluor 700	AF6896N-100UG
Alexa Fluor 750	AF6896S-100UG
Alexa Fluor Plus 405	AF6896AFP405-100UG
Alexa Fluor Plus 488	AF6896AFP488-100UG
Alexa Fluor Plus 555	AF6896AFP555-100UG
Alexa Fluor Plus 594	AF6896AFP594-100UG
Alexa Fluor Plus 647	AF6896AFP647-100UG
Alexa Fluor Plus 680	AF6896AFP680-100UG
Allophycocyanin	AF6896A
Biotin	AF6896B
CoraFluor 1	AF6896CL1
DyLight 350	AF6896D
DyLight 405	AF6896E
DyLight 488	AF6896K
DyLight 550	AF6896L
DyLight 594	AF6896M
DyLight 650	AF6896W
DyLight 680	AF6896Y
DyLight 755	AF6896Z
FITC	AF6896F
HRP	AF6896H
Janelia Fluor 525	AF6896JF525
Janelia Fluor 549	AF6896I
Janelia Fluor 646	AF6896J
Janelia Fluor 669	AF6896JF669
PE	AF6896P
PerCP	AF6896C
mFluor Violet 450 SE	AF6896MFV450
mFluor Violet 500 SE	AF6896MFV500
mFluor Violet 610 SE	AF6896MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6896-SP

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NBP2-79808

Vinculin Antibody (VCL/2575)

100 μg

NBP2-79808

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human A431, K562, NIH3T3 cell lysate using Vinculin Antibody (VCL/2575).

Protein Array: Vinculin Antibody (VCL/2575) [NBP2-79808] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2575) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-79942AF350
Alexa Fluor 405	NBP2-79942AF405
Alexa Fluor 488	NBP2-79942AF488
Alexa Fluor 532	NBP2-79942AF532
Alexa Fluor 594	NBP2-79942AF594
Alexa Fluor 647	NBP2-79942AF647
Alexa Fluor 700	NBP2-79942AF700
Alexa Fluor 750	NBP2-79942AF750
Alexa Fluor Plus 405	NBP2-79942AFP405
Alexa Fluor Plus 488	NBP2-79942AFP488
Alexa Fluor Plus 555	NBP2-79942AFP555
Alexa Fluor Plus 594	NBP2-79942AFP594
Alexa Fluor Plus 647	NBP2-79942AFP647
Alexa Fluor Plus 680	NBP2-79942AFP680
Allophycocyanin	NBP2-79942APC
Biotin	NBP2-79942B
CoraFluor 1	NBP2-79942CL1
DyLight 350	NBP2-79942UV
DyLight 405	NBP2-79942V
DyLight 488	NBP2-79942G
DyLight 550	NBP2-79942R
DyLight 594	NBP2-79942DL594
DyLight 650	NBP2-79942C
DyLight 680	NBP2-79942FR
DyLight 755	NBP2-79942IR
FITC	NBP2-79942F
HRP	NBP2-79942H
Janelia Fluor 525	NBP2-79942JF525
Janelia Fluor 549	NBP2-79942JF549
Janelia Fluor 646	NBP2-79942JF646
Janelia Fluor 669	NBP2-79942JF669
PE	NBP2-79942PE
PerCP	NBP2-79942PCP
mFluor Violet 450 SE	NBP2-79942MFV450
mFluor Violet 500 SE	NBP2-79942MFV500
mFluor Violet 610 SE	NBP2-79942MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-79942

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MAB68961

Vinculin Antibody (728513) [Unconjugated]

(1 Publication)

100 μg

MAB68961

NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Vinculin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Vinculin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB68961U-100UG
Alexa Fluor 405	FAB68961V-100UG
Alexa Fluor 488	FAB68961G-100UG
Alexa Fluor 532	FAB68961X-100UG
Alexa Fluor 594	FAB68961T-100UG
Alexa Fluor 647	FAB68961R-100UG
Alexa Fluor 700	FAB68961N-100UG
Alexa Fluor 750	FAB68961S-100UG
Alexa Fluor Plus 405	FAB68961AFP405-100UG
Alexa Fluor Plus 488	FAB68961AFP488-100UG
Alexa Fluor Plus 555	FAB68961AFP555-100UG
Alexa Fluor Plus 594	FAB68961AFP594-100UG
Alexa Fluor Plus 647	FAB68961AFP647-100UG
Alexa Fluor Plus 680	FAB68961AFP680-100UG
Allophycocyanin	FAB68961A
Biotin	FAB68961B
CoraFluor 1	FAB68961CL1
DyLight 350	FAB68961D
DyLight 405	FAB68961E
DyLight 488	FAB68961K
DyLight 550	FAB68961L
DyLight 594	FAB68961M
DyLight 650	FAB68961W
DyLight 680	FAB68961Y
DyLight 755	FAB68961Z
FITC	FAB68961F
HRP	FAB68961H
Janelia Fluor 525	FAB68961JF525
Janelia Fluor 549	FAB68961I
Janelia Fluor 646	FAB68961J
Janelia Fluor 669	FAB68961JF669
PE	FAB68961P
PerCP	FAB68961C
mFluor Violet 450 SE	FAB68961MFV450
mFluor Violet 500 SE	FAB68961MFV500
mFluor Violet 610 SE	FAB68961MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB68961-SP

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NBP2-41274

Vinculin Antibody - BSA Free

(1 Publication)

0.1 mg

NBP2-41274

Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41274 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] - Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-41274AF350
Alexa Fluor 405	NBP2-41274AF405
Alexa Fluor 488	NBP2-41274AF488
Alexa Fluor 532	NBP2-41274AF532
Alexa Fluor 594	NBP2-41274AF594
Alexa Fluor 647	NBP2-41274AF647
Alexa Fluor 700	NBP2-41274AF700
Alexa Fluor 750	NBP2-41274AF750
Alexa Fluor Plus 405	NBP2-41274AFP405
Alexa Fluor Plus 488	NBP2-41274AFP488
Alexa Fluor Plus 555	NBP2-41274AFP555
Alexa Fluor Plus 594	NBP2-41274AFP594
Alexa Fluor Plus 647	NBP2-41274AFP647
Alexa Fluor Plus 680	NBP2-41274AFP680
Allophycocyanin	NBP2-41274APC
Biotin	NBP2-41274B
CoraFluor 1	NBP2-41274CL1
DyLight 350	NBP2-41274UV
DyLight 405	NBP2-41274V
DyLight 488	NBP2-41274G
DyLight 550	NBP2-41274R
DyLight 594	NBP2-41274DL594
DyLight 650	NBP2-41274C
DyLight 680	NBP2-41274FR
DyLight 755	NBP2-41274IR
FITC	NBP2-41274F
HRP	NBP2-41274H
Janelia Fluor 525	NBP2-41274JF525
Janelia Fluor 549	NBP2-41274JF549
Janelia Fluor 646	NBP2-41274JF646
Janelia Fluor 669	NBP2-41274JF669
PE	NBP2-41274PE
PerCP	NBP2-41274PCP
mFluor Violet 450 SE	NBP2-41274MFV450
mFluor Violet 500 SE	NBP2-41274MFV500
mFluor Violet 610 SE	NBP2-41274MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-41274-0.025mg

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NBP3-16145

Vinculin Antibody (9I3Y8)

100 ul

NBP3-16145

Analysis of lysates from PC-12 cells using Vinculin Rabbit mAb at 1:200000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.

Analysis of various lysates using Vinculin Rabbit mAb at 1:50000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Vinculin Antibodies

Vinculin Antibody (hVIN-1)

Vinculin Antibody (728526) [Unconjugated]

Vinculin Antibody - Azide Free

Vinculin Antibody - BSA Free

Vinculin Antibody (VIN-11-5)

Vinculin Antibody - BSA Free

Vinculin Antibody - BSA Free

Vinculin Antibody [Unconjugated]

Vinculin Antibody (VCL/2575)

Vinculin Antibody (728513) [Unconjugated]

Vinculin Antibody - BSA Free

Vinculin Antibody (9I3Y8)