VASP Antibody (OTI4D6)

Images

 
Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human endometrium tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human liver tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymph node tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Hela cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Order Details

VASP Antibody (OTI4D6) Summary

Immunogen
Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
Marker
Focal Adhesion Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VASP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VASP Knockout HeLa Cell Lysate
Publications
Read Publication using NBP2-00555.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Purity
Affinity purified

Alternate Names for VASP Antibody (OTI4D6)

  • vasodilator-stimulated phosphoprotein
  • VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASP Antibody (NBP2-00555)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-00555 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VASP Antibody (NBP2-00555) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74830AF350
Alexa Fluor 405 NBP2-74830AF405
Alexa Fluor 488 NBP2-74830AF488
Alexa Fluor 532 NBP2-74830AF532
Alexa Fluor 594 NBP2-74830AF594
Alexa Fluor 647 NBP2-74830AF647
Alexa Fluor 700 NBP2-74830AF700
Alexa Fluor 750 NBP2-74830AF750
Allophycocyanin NBP2-74830APC
Allophycocyanin/Cy7 NBP2-74830APCCY7
Biotin NBP2-74830B
DyLight 350 NBP2-74830UV
DyLight 405 NBP2-74830V
DyLight 488 NBP2-74830G
DyLight 550 NBP2-74830R
DyLight 594 NBP2-74830DL594
DyLight 650 NBP2-74830C
DyLight 680 NBP2-74830FR
DyLight 755 NBP2-74830IR
FITC NBP2-74830F
HRP NBP2-74830H
Janelia Fluor 549 NBP2-74830JF549
Janelia Fluor 646 NBP2-74830JF646
PE NBP2-74830PE
PE/Atto594 NBP2-74830PEATT594
PE/Cy5.5 NBP2-74830PECY55
PE/Cy7 NBP2-74830PECY7
PerCP NBP2-74830PCP

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Bioinformatics

Gene Symbol VASP
Entrez