Home » FAK » FAK Antibodies » FAK Antibody (495919) [Unconjugated]

FAK Antibody (495919) [Unconjugated]

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Western blot shows lysates of MCF‑7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh‑7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Human FAK Monoclonal Antibody (Catalog # MAB4467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of MCF‑7 human breast cancer cell line, A549 human lung carcinoma cell line, Huh‑7 human hepatoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with ...read more
FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human FAK Monoclonal Antibody (Catalog # MAB4467) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAK ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KO
Clone
495919
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4467-SP
100% Guarantee on every product

FAK Antibody (495919) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397
Specificity
Detects human FAK in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTK2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
Control
FAK Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FAK Antibody (495919) [Unconjugated]

  • EC 2.7.10
  • FADK 1
  • FADK1
  • FADKFAK-related non-kinase polypeptide
  • FAK
  • FAK1FRNK
  • FAKEC 2.7.10.2
  • focal adhesion kinase 1
  • pp125FAK
  • Protein-tyrosine kinase 2
  • PTK2 protein tyrosine kinase 2
  • PTK2

Background

Focal adhesion kinase 1 (FAK), also known as FAK1 and PTK2, is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in focal adhesions. This cellular localization is directed by a C-terminal 125 amino acid "Focal Adhesion Targeting" (FAT) sequence. FAK plays an important role in migration, cell spreading, differentiation and apoptosis. It associates with several different signaling proteins, such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and Paxillin. These associations enable FAK to function within a network of integrin-stimulated signaling pathways, leading to the activation of targets such as the ERK and JNK mitogen-activated protein kinase pathways. Increased expression and/or activity of FAK in various cancers has been correlated with enhanced proliferation, migration and invasiveness of human tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

FAK Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol PTK2
Uniprot