Vimentin Antibody

Images

 
Genetic Strategies: Knockdown Validated: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.
Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.
Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.
Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.
Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.
Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit ...read more
Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: ...read more
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Analysis of paraffin-embedded RT2 xenograft, using Vimentin antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.
Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded mouse testis. Vimentin antibody at 1:500.
Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG ...read more
Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Eq, Bv, Ca, Ch, Pm, GpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Vimentin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Marker
Mesenchymal Cells Marker
Predicted Species
Canine (98%), Chicken (92%), Bovine (98%), Guinea Pig (97%), Chimpanzee (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:5000-1:50000
Application Notes
Sandwich ELISA- Assay dependent. Vimentin antibody validated for ICC/IF from a verified customer review.
Theoretical MW
53.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP1-31327 in the following applications:

Publications
Read Publications using
NBP1-31327 in the following applications:

  • 5 publications
  • WB
    5 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%). Equine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Vimentin Antibody

  • epididymis secretory sperm binding protein
  • FLJ36605
  • VIM
  • Vimentin

Background

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Eq, Bv, Ca, Ch, Pm, Gp
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA, KD

Publications for Vimentin Antibody (NBP1-31327)(10)

We have publications tested in 3 confirmed species: Human, Bovine, Guinea pig.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(5)
WB
(5)
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Filter By Species
Human
(4)
Bovine
(2)
Guinea pig
(1)
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Showing Publications 1 - 10 of 10.
Publications using NBP1-31327 Applications Species
Huidrom LD, Dhanaji SN, Pandey S et al. Embryo-Uterine Cross-Talk: Exploration of the Immunomodulatory Mechanism in Buffalo Animals : an open access journal from MDPI 2022 Nov 14 [PMID: 36428366] (ICC/IF, Bovine) ICC/IF Bovine
Nagel A, Popeda M, Muchlinska A et al. ERalpha36-High Cancer-Associated Fibroblasts as an Unfavorable Factor in Triple-Negative Breast Cancer Cancers Apr 15 2022 [PMID: 35454913]
She M, Li T, Shi W et al. AREG is involved in scleral remodeling in form-deprivation myopia via the ERK1/2-MMP-2 pathway FASEB journal : official publication of the Federation of American Societies for Experimental Biology May 1 2022 [PMID: 35436023] (ICC/IF, Guinea pig) ICC/IF Guinea pig
H LD, D SN, Pandey S et al. Impact of uterine epithelial cells and its conditioned medium on the in vitro embryo production in buffalo (Bubalusbubalis) Theriogenology Feb 18 2022 [PMID: 35219005] (ICC/IF, Bovine) ICC/IF Bovine
Li Z, Zhou Z, Wu X et al. LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance Am J Cancer Res Jul 1 2020 [PMID: 32775002] (WB, ICC/IF, Human) WB, ICC/IF Human
Zhou Z, Zhou Q, Wu X, et al. VCAM-1 Secreted from Cancer-Associated Fibroblasts Enhances the Growth and Invasion of Lung Cancer Cells through AKT and MAPK Signaling Cancer Letters Jan 1 2020 [PMID: 31904479] (WB) WB
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. Feb 8 2019 [PMID: 30736829] (WB, Human) WB Human
Zuppinger C, Gibbons G et al. Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. Eur J Histochem Jun 21 2017 [PMID: 28735524] (ICC/IF, Human) ICC/IF Human
Inamdar SM, Hsu SC, Yeaman C. Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies. Front Cell Dev Biol 2016 Jun 03 [PMID: 27376061] (WB) WB
Cho CK, Smith CR, Diamandis EP. Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery. J Proteome Res 2010 Jul [PMID: 20459121] (WB, Human) WB Human

Reviews for Vimentin Antibody (NBP1-31327) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Equine.

Reviews using NBP1-31327:
Filter by Applications
ICC
(1)
IF
(1)
WB
(1)
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Filter by Species
Human
(2)
Equine
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Vimentin NBP1-31327
Enlarge
5
reviewed by:
Verified Customer
ICC Equine 12/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesEquine
Lot?

Comments

CommentsI will have to look up the lot number and supply tomorrow.
Staining material: cellular aggregates composed of equine endometrial fibroblast only.
Co-staining using vimentin antibody (purchase order number: 3641031 from NovusBio website directly) and a cytokeratin antibody (mouse monoclonal) we have in the lab. Equine endometrial fibroblast express vimentin only.
The image on the left shows the staining for vimentin and DAPI. Cytoplasmic staining is evident colored in red. Nuclei are stained with DAPI colored in blue. No staining for vimentin present. The images on the right hand site depict the negative control (i.e. omission of the primary antibody, DAPI only).
Immunofluorescence Vimentin NBP1-31327
Enlarge
5
reviewed by:
Verified Customer
IF Human 07/13/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedprimary human cells
SpeciesHuman
Lot42095
  5
reviewed by:
Haiquan Lu
WB Human 09/02/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vimentin Antibody (NBP1-31327) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Vimentin. Showing 1-10 of 14 blog posts - Show all blog posts.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.


  Read full blog post.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
12/11/2018
Application: ICC
Species: Equine

Verified Customer
07/13/2016
Application: IF
Species: Human

 
Haiquan Lu
09/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol VIM