Vimentin Antibodies
We offer Vimentin Antibodies for use in common research applications: ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each Vimentin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Vimentin Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Feline, Goat, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep. Use the list below to choose the Vimentin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Vimentin polyclonal antibodies and browse our Vimentin monoclonal antibody catalog.
Alternate Names for Vimentin Antibodies
anti-Vimentin antibody, anti-VIM antibody, anti-FLJ36605 antibody
29 Results for "vimentin" in Primary Antibodies
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg "Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0004.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Frozen-NB300-223-img0003.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - Infarcted mouse heart frozen sections stained with vimentin antibody at 1:200. Secondary antibody at 1:500. Fixation with 4% PFA. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Frozen-NB300-223-img0005.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Attenuated reactive astrocytosis after stroke in Smad1 cKO mice. Enlarged IHC images of boxed areas at the cortical peri-infarct area with the indicated reactive astrocyte markers GFAP, Nestin, and Vimentin. Enlarged images of GFAP IHC highlight the hypertrophic morphology of GFAP+ astrocytes in mutants. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0136967), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0006.jpg)
![Knockout Validated: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Knockdown-Validated-NBP1-92687-img0011.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0003.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of different cell lysates using mouse mAb to vimentin, NBP1-92687, dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Western-Blot-NBP1-92687-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin, NBP1-92687, dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0010.jpg)
![Immunohistochemistry: Vimentin Antibody (2D1) [NBP1-92687] - Mouse cortex microslice section (from postnatal day 3) was immunostained with (1:500 dil.). This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Vimentin Antibody (2D1)-Immunohistochemistry-NBP1-92687-img0005.jpg)
![Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Simple-Western-NBP1-92687-img0004.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0032.jpg "Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image from verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-NBP1-31327-img0037.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0036.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0026.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0027.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0029.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0028.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Analysis of paraffin-embedded RT2 xenograft, using Vimentin antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0033.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0034.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded mouse testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0035.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV203) [NBP1-97671] - ICC for dog cells. The dog blood cells on the slide were incubated with the antibody, washed and mounted with a anti fade mounting media and read under fluorescent microscope. This image was submitted by customer review. Image from the Alexa Fluor 405 version of this antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunocytochemistry-Immunofluorescence-NBP1-97671-img0004.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV203) [NBP1-97671] - ICC for dog cells. The dog blood cells on the slide were incubated with the antibody, washed and mounted with a anti fade mounting media and read under fluorescent microscope. This image was submitted by customer review. Image from the Alexa Fluor 405 version of this antibody.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors. Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunohistochemistry-Paraffin-NBP1-97671-img0007.jpg)
![Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with Vimentin Antibody (RV203) NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Flow-Cytometry-NBP1-97671-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunohistochemistry-Paraffin-NBP1-97671-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunohistochemistry-Frozen-NBP1-97671-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunohistochemistry-Frozen-NBP1-97671-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors. Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection). TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV203)-Immunohistochemistry-Paraffin-NBP1-97671-img0006.jpg)
![Knockout Validated: Vimentin Antibody [NBP1-85814] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Rabbit Anti-Human Vimentin Polyclonal Antibody (Catalog # NBP1-85814) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Knockout-Validated-NBP1-85814-img0022.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody [NBP1-85814] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Rabbit Anti-Human Vimentin Polyclonal Antibody (Catalog # NBP1-85814) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0023.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-85814] - Analysis in human cell lines U-251MG and MCF-7 using anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-85814-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85814-img0021.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0027.jpg)
![Western Blot: Vimentin Antibody [NBP1-85814] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-85814-img0013.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0025.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0026.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0028.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0006.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0007.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0008.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Immunohistochemistry-Paraffin-NBP2-29433-img0005.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Western-Blot-NBP2-29433-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM452)-Protein-Array-NBP2-29433-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo")
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0005.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - MUB1900L1: Frozen section of swine colon immunostained with RV202-FITC (1:200)](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon immunostained with RV202-HRP (1:200)](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV202) [NBP1-97672] - Paraffin section of human colon](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Paraffin-NBP1-97672-img0004.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0012.jpg "Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (812) [NBP2-43560] - Paraffin-embedded rat ovary. Vimentin antibody [812] diluted at 1:250.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Paraffin-NBP2-43560-img0009.jpg)
![Flow Cytometry: Vimentin Antibody (812) [NBP2-43560] - An intracellular stain was performed on Jurkat cells with Vimentin Antibody (812) NBP2-43560AF00 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Flow-Cytometry-NBP2-43560-img0010.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812].](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0004.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:3000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0005.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:5000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0007.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] ) diluted at 1:75000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0008.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Frozen-NBP2-43560-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Frozen-NBP2-43560-img0006.jpg)
![Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Sandwich-ELISA-NBP2-43560-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin Antibody (V9)-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY licence.")
![Immunohistochemistry-Frozen: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in a frozen tissue section of human colon. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(V9)-Immunohistochemistry-Frozen-NBP1-97670-img0005.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - An intracellular stain was performed on Jurkat cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(V9)-Flow-Cytometry-NBP1-97670-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of glomeruli and connetive tissue in human kidney with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(V9)-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in swine colon with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(V9)-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0004.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - Analysis of PE conjugate of NBP1-97670. An intracellular stain was performed on THP-1 cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(V9)-Flow-Cytometry-NBP1-97670-img0001.jpg)
![Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Western-Blot-NBP2-44833-img0008.jpg "Western Blot: Vimentin Antibody (VM1170) [NBP2-44833] - Western Blot Analysis of HEP2 cell lysate using Vimentin Antibody (VM1170).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM1170) [NBP2-44833] - Human Uterus stained with Vimentin Monoclonal Antibody (VM1170).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(VM1170)-Immunohistochemistry-Paraffin-NBP2-44833-img0006.jpg)