Vimentin Antibodies
We offer Vimentin Antibodies for use in common research applications: ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each Vimentin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Vimentin Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Feline, Goat, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep. Use the list below to choose the Vimentin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Vimentin polyclonal antibodies and browse our Vimentin monoclonal antibody catalog.
Alternate Names for Vimentin Antibodies
anti-Vimentin antibody, anti-VIM antibody, anti-FLJ36605 antibody
28 Results for "vimentin" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0001.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.")
![Western Blot: Vimentin Antibody [NB300-223] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with NB300-223, showing a single strong clean band at approx. 50kDa.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0032.jpg "Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0028.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - A. 20 ug 293T whole cell lysate/extract. B. 10 ug 293T whole cell lysate/extract. C. 5 ug 293T whole cell lysate/extract. D. 1 ug 293T whole cell lysate/extract.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0026.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0027.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0029.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:20000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0025.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin protein at cytosol on mouse thymus gland.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0012.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody [NBP1-31327] - Immunofluorescence photomicrographs of frozen sections of mouse brain. Green: Vimentin antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Frozen-NBP1-31327-img0017.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0020.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot of crude extract HeLa. Lane 18 was probed with NBP1-92687 antibody. Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with a monoclonal antibody to Vimentin (cat# NBP1-92688). Lane 15 was probed with an antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and Lane 16 with NBP1-92691, an antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.](http://images.novusbio.com/fullsize/Vimentin-Antibody-(2D1)-Western-Blot-NBP1-92687-img0001.jpg "Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot of crude extract HeLa. Lane 18 was probed with NBP1-92687 antibody. Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with a monoclonal antibody to Vimentin (cat# NBP1-92688). Lane 15 was probed with an antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and Lane 16 with NBP1-92691, an antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0003.jpg)
![Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(2D1)-Simple-Western-NBP1-92687-img0004.jpg)
![Western Blot: Vimentin Antibody (SP20) [NBP2-12472] - Hela](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Western-Blot-NBP2-12472-img0005.jpg "Western Blot: Vimentin Antibody (SP20) [NBP2-12472] - Hela")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Renal Cell Carcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0013.jpg)
![Flow Cytometry: Vimentin Antibody (SP20) [NBP2-12472] - Analysis of rabbit anti-Vimentin (SP20) antibody in HeLa (green) compare to negative control of rabbit IgG (blue)](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Flow-Cytometry-NBP2-12472-img0006.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Melanoma](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human tosil stained with anti-vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Colon Adencarcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Prostate Adenocarcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0009.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Lung Squamous Cell Carcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Ovary Adenocarcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0011.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (SP20) [NBP2-12472] - Human Stomach Adenocarcinoma stained with anti-Vimentin antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(SP20)-Immunohistochemistry-Paraffin-NBP2-12472-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo")
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV202) [NBP1-97672] - Paraffin section of human colon](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Paraffin-NBP1-97672-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0005.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - MUB1900L1: Frozen section of swine colon immunostained with RV202-FITC (1:200)](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon immunostained with RV202-HRP (1:200)](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(RV202)-Immunohistochemistry-Frozen-NBP1-97672-img0003.jpg)
![Western Blot: Vimentin Antibody [NBP1-85814] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Western Blot-NBP1-85814-img0013.jpg "Western Blot: Vimentin Antibody [NBP1-85814] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85814-img0012.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human rectum shows strong positivity in tissue stroma.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0011.jpg)
![Western Blot: Vimentin Antibody [NBP1-85814] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NBP1-85814-img0009.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human duodenum shows distinct positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0003.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0006.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0007.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP1-85814-img0008.jpg)
![Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [7812] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Western-Blot-NBP2-43687-img0013.jpg "Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [7812] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (7812) [NBP2-43687] - HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: Vimentin protein stained by Vimentin antibody [7812] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Immunocytochemistry-Immunofluorescence-NBP2-43687-img0011.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (7812) [NBP2-43687] - Paraffin-embedded human lung carcinoma. Vimentin antibody [7812] diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Immunohistochemistry-Paraffin-NBP2-43687-img0014.jpg)
![Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [7812] dilution: 1:1000](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Western-Blot-NBP2-43687-img0005.jpg)
![Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [7812] dilution: 1:1000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Western-Blot-NBP2-43687-img0006.jpg)
![Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [7812] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Western-Blot-NBP2-43687-img0008.jpg)
![Western Blot: Vimentin Antibody (7812) [NBP2-43687] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [7812] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Western-Blot-NBP2-43687-img0010.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (7812) [NBP2-43687] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [7812] diluted at 1:1000. Blue: DAPI.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Immunohistochemistry-Frozen-NBP2-43687-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (7812) [NBP2-43687] - Analysis of human breast carcinoma. Vimentin antibody [7812] diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Immunohistochemistry-Paraffin-NBP2-43687-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (7812) [NBP2-43687] - Analysis of human endometrial carcinoma. Vimentin antibody [7812] diluted at 1:500.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(7812)-Immunohistochemistry-Paraffin-NBP2-43687-img0004.jpg)
![Western Blot: Vimentin Antibody [NBP2-25165] - Blot of crude extract of HeLa cells, showing a single strong clean band at 55 kDa.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Western Blot-NBP2-25165-img0001.jpg "Western Blot: Vimentin Antibody [NBP2-25165] - Blot of crude extract of HeLa cells, showing a single strong clean band at 55 kDa.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP2-25165] - View of HeLa cells stained with NBP2-25165 (red) and counterstained with monoclonal antibody to Lamin A + C, NBP2-25151 (green). DNA is blue. NBP2-25165 reveals strong cytoplasmic intermediate filament staining, while NBP2-25151 antibody reveals strong nuclear lamina staining.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25165-img0003.jpg)
![Simple Western: Vimentin Antibody [NBP2-25165] - Simple Western lane view shows a specific band for Vimentin in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Simple-Western-NBP2-25165-img0004.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] ) diluted at 1:75000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0008.jpg "Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] ) diluted at 1:75000.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (812) [NBP2-43560] - Paraffin-embedded rat ovary. Vimentin antibody [812] diluted at 1:250.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Paraffin-NBP2-43560-img0009.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0002.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0003.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] diluted at 1:5000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0004.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:3000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0005.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [812] diluted at 1:5000.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Western-Blot-NBP2-43560-img0007.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Frozen-NBP2-43560-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(812)-Immunohistochemistry-Frozen-NBP2-43560-img0006.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Western-Blot-NB100-74564-img0002.jpg "Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.")
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-(J144)-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)