ETFA Antibody

Images

 
Western Blot: ETFA Antibody [NBP2-16382] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with ...read more
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Western Blot: ETFA Antibody [NBP2-16382] - 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.88 mg/ml

Order Details

ETFA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ETFA Antibody

  • alpha-ETF
  • electron transfer flavoprotein alpha-subunit
  • Electron transfer flavoprotein subunit alpha, mitochondrial
  • electron-transfer-flavoprotein, alpha polypeptide
  • EMA
  • GA2
  • glutaric aciduria II
  • MADD

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETFA
Uniprot