VEGFR1/Flt-1 Antibody [DyLight 405] Summary
A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.
Type 1 membrane protein (isoform Flt1)
Endothelial Cell Marker
This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations
Store at 4C in the dark.
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified
- Western Blot 1:100-1:2000
This VEGF Receptor 1 antibody is useful for Western blot, where a band is seen at ~180-230 kDa (the theoretical molecular weight of mouse VEGFR1 is ~150 kDa and human VEGFR1 is ~151 kDa). The difference in MWs is likely due to glycosylation or other post translational modifications. With CSF-1R/VEGFR2 chimera transfected lysates a doublet is seen at ~150 kDa representing VEGFR-2. An unknown band is also seen at ~50 kDa. Optimal working dilutions should be determined by the investigator.
Immunogen sequence has 100% homology to mouse and rat.
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for VEGFR1/Flt-1 Antibody [DyLight 405]
- EC 2.7.10
- EC 18.104.22.168
- Fms-like tyrosine kinase 1
- fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
- Tyrosine-protein kinase FRT
- Tyrosine-protein kinase receptor FLT
- vascular endothelial growth factor receptor 1
- Vascular permeability factor receptor
VEGF Receptor 1 (vascular endothelial growth factor receptor 1 or VEGFR1; also called Flt1 in mouse) is a tyrosine-protein kinase which acts as a cell-surface receptor for VEGFA, VEGFB and PGF ligands for playing an essential role in the embryonic vasculature development and regulation of angiogenesis (normal as well as cancerous tissues), cell survival, migration, macrophage function, chemotaxis, and tumor invasion. VEGFR1 is a 180-185 kD glycoprotein expressed primarily in vascular endothelial cells and also found in a wide range of non-endothelial cells, such as monocytes and macrophages, human trophoblasts, renal mesangial cells, vascular smooth muscle cells, dendritic cells and different human tumour cell types. Interestingly, VEGFR1 acts as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, whereas it promotes endothelial cell proliferation, survival and angiogenesis in adults. VEGFR1 expression is regulated by hypoxia through HREs in VEGFR1 promoter. It exists in an inactive conformation in the absence of bound ligand and binding of VEGFA, VEGFB or PGF leads to its dimerization followed by tyrosine residue autophosphorylation mediated activation. VEGFR1 phosphorylation sites includes Tyr794, Tyr1169, Tyr1213, Tyr1242, Tyr1327, Tyr1333 etc. and VEGFR1 phosphorylation pattern depends on the ligand, e.g. PlGF, but not VEGFA, induces phosphorylation of Tyr1309. VEGFR1 has been shown to exert its diverse biological activities through its effects on multiple signal transduction pathways including VEGF, KDR, PLCG, PI3K, MAPK etc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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