VEGFR3/Flt-4 Antibody [Unconjugated]

Images

 
bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGFR3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # ...read more
Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old ...read more
Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Endothelial changes after pericyte depletion. a–f Maximum intensity projection of confocal images from control and DTRiPC P6 retinas stained for IB4 (red) in combination with VEGF-A a, VEGFR2 b, VEGFR3 c, Tie2 d, Esm1 ...read more
Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Cultured murine LECs express VDR.To check lymphatic origin of mouse LECs, we used three different well-established markers expressed by LECs. (a) Lymphatic origin of murine LECs was confirmed by IHC for Prox-1, VEGFR3 ...read more
Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Kinase activity of EphB4 is required for lymphatic valve development.(a) Visualization of mesenteric lymphatic vessels and valves (arrows) by staining for Prox-1 and VEGFR3 2 days following treatment of P3 neonatal mice ...read more
Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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View Available Formulations

VEGFR3/Flt-4 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGFR3/Flt-4
Tyr25-Asp770
Accession # P35917
Specificity
Detects mouse VEGFR3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) VEGFR3 is observed and less than 5% cross-reactivity with recombinant mouse VEGFR2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
AF743 in the following applications:

Publications
Read Publications using
AF743 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR3/Flt-4 Antibody (AF743)(74)

We have publications tested in 3 confirmed species: Mouse, Equine, Transgenic Mouse.

We have publications tested in 8 applications: ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(5)
IHC
(50)
IHC-Fr
(12)
IHC-P
(8)
Immunoprecipitation
(2)
Western Blot
(6)
All Applications
Filter By Species
Mouse
(72)
Equine
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using AF743 Applications Species
Iga, T;Kobayashi, H;Kusumoto, D;Sanosaka, T;Fujita, N;Tai-Nagara, I;Ando, T;Takahashi, T;Matsuo, K;Hozumi, K;Ito, K;Ema, M;Miyamoto, T;Matsumoto, M;Nakamura, M;Okano, H;Shibata, S;Kohyama, J;Kim, KK;Takubo, K;Kubota, Y; Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis Nature cell biology 2023-10-05 [PMID: 37798545] (IHC, Mouse) IHC Mouse
Uçar, MC;Hannezo, E;Tiilikainen, E;Liaqat, I;Jakobsson, E;Nurmi, H;Vaahtomeri, K; Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks Nature communications 2023-09-21 [PMID: 37735168] (IHC, Mouse) IHC Mouse
Jannaway, M;Iyer, D;Mastrogiacomo, DM;Li, K;Sung, DC;Yang, Y;Kahn, ML;Scallan, JP; VEGFR3 is required for button junction formation in lymphatic vessels Cell reports 2023-07-15 [PMID: 37454290] (IHC, Mouse) IHC Mouse
Freise, L;Behncke, RY;Allerkamp, HH;Sandermann, TH;Chu, NH;Funk, EM;Hondrich, LJ;Riedel, A;Witzel, C;Hansmeier, NR;Danyel, M;Gellhaus, A;Dechend, R;Hägerling, R; Three-Dimensional Histological Characterization of the Placental Vasculature Using Light Sheet Microscopy Biomolecules 2023-06-17 [PMID: 37371590] (IHC, Mouse) IHC Mouse
Qi, L;Li, X;Zhang, F;Zhu, X;Zhao, Q;Yang, D;Hao, S;Li, T;Li, X;Tian, T;Feng, J;Sun, X;Wang, X;Gao, S;Wang, H;Ye, J;Cao, S;He, Y;Wang, H;Wei, B; VEGFR-3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection Cell reports 2023-05-10 [PMID: 37167063] (Flow Cytometry, Mouse) Flow Cytometry Mouse
J Kazenwadel, P Venugopal, A Oszmiana, J Toubia, L Arriola-Ma, V Panara, SG Piltz, C Brown, W Ma, AW Schreiber, K Koltowska, S Taoudi, PQ Thomas, HS Scott, NL Harvey A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature Nature, 2023-01-25;614(7947):343-348. 2023-01-25 [PMID: 36697821] (IHC, Mouse) IHC Mouse
K Takahashi, K Abe, SI Kubota, N Fukatsu, Y Morishita, Y Yoshimatsu, S Hirakawa, Y Kubota, T Watabe, S Ehata, HR Ueda, T Shimamura, K Miyazono An analysis modality for vascular structures combining tissue-clearing technology and topological data analysis Nature Communications, 2022-09-12;13(1):5239. 2022-09-12 [PMID: 36097010] (IHC, Mouse) IHC Mouse
X Li, L Qi, D Yang, S Hao, F Zhang, X Zhu, Y Sun, C Chen, J Ye, J Yang, L Zhao, DM Altmann, S Cao, H Wang, B Wei Meningeal lymphatic vessels mediate neurotropic viral drainage from the central nervous system Nature Neuroscience, 2022-05-06;25(5):577-587. 2022-05-06 [PMID: 35524140] (IHC, Mouse) IHC Mouse
D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-11-11;0(0):e12924. 2021-11-11 [PMID: 34762341] (IHC, Mouse) IHC Mouse
E Redder, N Kirschnick, S Bobe, R Hägerling, NR Hansmeier, F Kiefer Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities PLoS ONE, 2021-09-20;16(9):e0249256. 2021-09-20 [PMID: 34543279] (IHC, Mouse) IHC Mouse
Show All 74 Publications.

Reviews for VEGFR3/Flt-4 Antibody (AF743) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF743:
Filter by Applications
WB
(1)
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19901262
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23878309
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19901262
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for VEGFR3/Flt-4 Antibody (AF743) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

 
Verified Customer
01/09/2015
Application: WB
Species: Mouse

 
Verified Customer
01/09/2015
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol FLT4
Uniprot