VEGFR3/Flt-4 Antibody [Unconjugated]

Images

 
bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGFR3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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View Available Formulations

VEGFR3/Flt-4 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGFR3/Flt-4
Tyr25-Asp770
Accession # P35917
Specificity
Detects mouse VEGFR3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) VEGFR3 is observed and less than 5% cross-reactivity with recombinant mouse VEGFR2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
AF743 in the following applications:

Publications
Read Publications using
AF743 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR3/Flt-4 Antibody (AF743)(65)

We have publications tested in 2 confirmed species: Mouse, Equine.

We have publications tested in 7 applications: ELISA Development, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(3)
IHC
(35)
IHC-Fr
(11)
IHC-P
(7)
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Mouse
(63)
Equine
(1)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using AF743 Applications Species
D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021;0(0):e12924. 2021 [PMID: 34762341] (IHC, Mouse) IHC Mouse
E Redder, N Kirschnick, S Bobe, R Hägerling, NR Hansmeier, F Kiefer Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities PLoS ONE, 2021;16(9):e0249256. 2021 [PMID: 34543279] (IHC, Mouse) IHC Mouse
A Chakrabort, R Upadhya, TA Usman, AK Shetty, JM Rutkowski Chronic VEGFR-3 signaling preserves dendritic arborization and sensitization under stress Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 34389489] (IHC, Mouse) IHC Mouse
L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34343491] (IHC, Mouse) IHC Mouse
D Zhang, J Huang, X Sun, H Chen, S Huang, J Yang, X Du, Q Tan, F Luo, R Zhang, S Zhou, W Jiang, Z Ni, Z Wang, M Jin, M Xu, F Li, L Chen, M Liu, N Su, X Luo, L Yin, Y Zhu, JQ Feng, D Chen, H Qi, L Chen, Y Xie Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway Nature Communications, 2021;12(1):4391. 2021 [PMID: 34282140] (IHC, Mouse) IHC Mouse
KP Yeo, HY Lim, CH Thiam, SH Azhar, C Tan, Y Tang, WQ See, XH Koh, MH Zhao, ML Phua, A Balachande, Y Tan, SY Lim, HS Chew, LG Ng, V Angeli Efficient aortic lymphatic drainage is necessary for atherosclerosis regression induced by ezetimibe Science Advances, 2020;6(50):. 2020 [PMID: 33310846] (IHC, Mouse) IHC Mouse
I Tai-Nagara, Y Hasumi, D Kusumoto, H Hasumi, K Okabe, T Ando, F Matsuzaki, F Itoh, H Saya, C Liu, W Li, YS Mukouyama, W Marston Li, X Liu, M Hirashima, Y Suzuki, S Funasaki, Y Satou, M Furuya, M Baba, Y Kubota Blood and lymphatic systems are segregated by the FLCN tumor suppressor Nature Communications, 2020;11(1):6314. 2020 [PMID: 33298956] (IHC, Mouse) IHC Mouse
SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020;11(1):4102. 2020 [PMID: 32796823] (Western Blot, Mouse) Western Blot Mouse
SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020;11(1):4102. 2020 [PMID: 32796823] (Western Blot, Mouse) Western Blot Mouse
KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32182215] (Western Blot, Mouse) Western Blot Mouse
Show All 65 Publications.

Reviews for VEGFR3/Flt-4 Antibody (AF743) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF743:
Filter by Applications
Western Blot
(1)
Immunohistochemistry-Frozen
(1)
Immunofluorescence
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
Western Blot Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19901262
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunohistochemistry-Frozen Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23878309
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19901262
SpeciesMouse

Product General Protocols

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Video Protocols

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FAQs for VEGFR3/Flt-4 Antibody (AF743) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for VEGFR3/Flt-4 Antibody (AF743)

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

 
Anonymous -
01/09/2015
Application: Western Blot
Species: Mouse

 
Anonymous -
01/09/2015
Application: Immunohistochemistry-Frozen
Species: Mouse

 
Anonymous -
01/09/2015
Application: Immunofluorescence
Species: Mouse

Bioinformatics

Gene Symbol FLT4
Uniprot