VEGFR1/Flt-1 Antibody

Images

 
Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The ...read more
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

VEGFR1/Flt-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [Uniprot: P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.
Localization
Type 1 membrane protein (isoform Flt1)
Marker
Endothelial Cell Marker
Specificity
This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Predicted Species
Mouse (100%), Rat (100%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
  • Western Blot 1:100 - 1:2000
Publications
Read Publications using
NB100-527 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VEGFR1/Flt-1 Antibody

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

VEGF Receptor 1 (vascular endothelial growth factor receptor 1 or VEGFR1; also called Flt1 in mouse) is a tyrosine-protein kinase which acts as a cell-surface receptor for VEGFA, VEGFB and PGF ligands for playing an essential role in the embryonic vasculature development and regulation of angiogenesis (normal as well as cancerous tissues), cell survival, migration, macrophage function, chemotaxis, and tumor invasion. VEGFR1 is a 180-185 kD glycoprotein expressed primarily in vascular endothelial cells and also found in a wide range of non-endothelial cells, such as monocytes and macrophages, human trophoblasts, renal mesangial cells, vascular smooth muscle cells, dendritic cells and different human tumour cell types. Interestingly, VEGFR1 acts as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, whereas it promotes endothelial cell proliferation, survival and angiogenesis in adults. VEGFR1 expression is regulated by hypoxia through HREs in VEGFR1 promoter. It exists in an inactive conformation in the absence of bound ligand and binding of VEGFA, VEGFB or PGF leads to its dimerization followed by tyrosine residue autophosphorylation mediated activation. VEGFR1 phosphorylation sites includes Tyr794, Tyr1169, Tyr1213, Tyr1242, Tyr1327, Tyr1333 etc. and VEGFR1 phosphorylation pattern depends on the ligand, e.g. PlGF, but not VEGFA, induces phosphorylation of Tyr1309. VEGFR1 has been shown to exert its diverse biological activities through its effects on multiple signal transduction pathways including VEGF, KDR, PLCG, PI3K, MAPK etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (NB100-527)(3)

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Product General Protocols

View specific protocols for VEGFR1/Flt-1 Antibody (NB100-527): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGFR1/Flt-1 Antibody (NB100-527). (Showing 1 - 2 of 2 FAQs).

  1. I am studying the regulation of PLCg during fertilization in the starfish. I am interested in trying your anti-FLT1 polyclonal antibody. Your information sheet says that the immunogen for this antibody is "A synthetic peptide corresponding to a sequence in the middle region of human FLT1". Since I will be using this antibody in starfish eggs, I compared the starfish protein sequence to the human protein sequence and found areas of high identity between the two and I want to use an antibody that is expected to bind to amino acids common to both. Therefore, I would like some more information to match the immunogen to the starfish protein. Can you tell me specifically which amino acids of human FLT1 were used to make the antibody?
    • The immunogen sequence to this product is proprietary. I can tell you it falls between amino acids 800-900 of the mouse FLT1 protein [UniProt# P35969].
  2. Would you be able to tell me the concentration of the current lot of NB100-527 - VEGFR1/VEGFR2 Ab?
    • The concentration of NB100-527 is 1mg/mL

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-527AF350
Alexa Fluor 405 NB100-527AF405
Alexa Fluor 488 NB100-527AF488
Alexa Fluor 532 NB100-527AF532
Alexa Fluor 594 NB100-527AF594
Alexa Fluor 647 NB100-527AF647
Alexa Fluor 700 NB100-527AF700
Alexa Fluor 750 NB100-527AF750
Biotin NB100-527B
DyLight 350 NB100-527UV
DyLight 405 NB100-527V
DyLight 488 NB100-527G
DyLight 550 NB100-527R
DyLight 594 NB100-527DL594
DyLight 650 NB100-527C
DyLight 680 NB100-527FR
DyLight 755 NB100-527IR
FITC NB100-527F
HRP NB100-527H
Janelia Fluor 549 NB100-527JF549
Janelia Fluor 646 NB100-527JF646

Additional VEGFR1/Flt-1 Products

Bioinformatics Tool for VEGFR1/Flt-1 Antibody (NB100-527)

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Diseases for VEGFR1/Flt-1 Antibody (NB100-527)

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PTMs for VEGFR1/Flt-1 Antibody (NB100-527)

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Bioinformatics

Gene Symbol FLT1