VEGFR1/Flt-1 Antibody - BSA Free

Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.

VEGFR1 overexpression in K562 cells. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VEGFR1/Flt-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VEGFR1/Flt-1 Antibody - BSA Free (NB100-527) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-VEGFR1/Flt-1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [Uniprot: P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.
• Localization: Type 1 membrane protein (isoform Flt1)
• Marker: Endothelial Cell Marker
• Specificity: This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VEGFR1/Flt-1 Antibody - BSA Free

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGF Receptor 1 (vascular endothelial growth factor receptor 1 or VEGFR1; also called Flt1 in mouse) is a tyrosine-protein kinase which acts as a cell-surface receptor for VEGFA, VEGFB and PGF ligands for playing an essential role in the embryonic vasculature development and regulation of angiogenesis (normal as well as cancerous tissues), cell survival, migration, macrophage function, chemotaxis, and tumor invasion. VEGFR1 is a 180-185 kD glycoprotein expressed primarily in vascular endothelial cells and also found in a wide range of non-endothelial cells, such as monocytes and macrophages, human trophoblasts, renal mesangial cells, vascular smooth muscle cells, dendritic cells and different human tumour cell types. Interestingly, VEGFR1 acts as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, whereas it promotes endothelial cell proliferation, survival and angiogenesis in adults. VEGFR1 expression is regulated by hypoxia through HREs in VEGFR1 promoter. It exists in an inactive conformation in the absence of bound ligand and binding of VEGFA, VEGFB or PGF leads to its dimerization followed by tyrosine residue autophosphorylation mediated activation. VEGFR1 phosphorylation sites includes Tyr794, Tyr1169, Tyr1213, Tyr1242, Tyr1327, Tyr1333 etc. and VEGFR1 phosphorylation pattern depends on the ligand, e.g. PlGF, but not VEGFA, induces phosphorylation of Tyr1309. VEGFR1 has been shown to exert its diverse biological activities through its effects on multiple signal transduction pathways including VEGF, KDR, PLCG, PI3K, MAPK etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (NB100-527)(5)

Publications using NB100-527

• Nascimento JJAC, Machado ASD, Della-Santa GML et al. Effects of photobiomodulation therapy on functional recovery, angiogenesis and redox status in denervated muscle of rats Einstein (Sao Paulo, Brazil) 2021-09-13 [PMID: 34586157] (IF/IHC, Rat)
• Su CC. Tanshinone IIA can inhibit MiaPaCa 2 human pancreatic cancer cells by dual blockade of the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways. Oncol. Rep. 2018-11-01 [PMID: 30226540] (WB, Human)
• Podemska-Jedrzejczak Z, Malinska A, et al. Vascular restenosis in coronary artery bypass grafting might be associated with VEGF-C/VEGFR-3 signaling pathway. Heart Vessels 2018-09-01 [PMID: 29557990] (IF/IHC, Human)
• Ptaszynska MM, Pendrak ML, Bandle RW, Stracke ML, Roberts DD. Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells. Mol Cancer Res;6(3):352-63. 2008-03-01 [PMID: 18337445] (WB, Human)
• Puglisi F, Puppin C, Pegolo E et al. Expression of periostin in human breast cancer. J Clin Pathol;614:494-8. 2008-04-01 [PMID: 17938160]

Review for VEGFR1/Flt-1 Antibody (NB100-527) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/19/2025

Summary

• Application: Western Blot
• Sample Tested: k562 cells
• Species: Human
• Lot: D161614-3

FAQs for VEGFR1/Flt-1 Antibody (NB100-527). (Showing 1 - 2 of 2 FAQs).

1. I am studying the regulation of PLCg during fertilization in the starfish. I am interested in trying your anti-FLT1 polyclonal antibody. Your information sheet says that the immunogen for this antibody is "A synthetic peptide corresponding to a sequence in the middle region of human FLT1". Since I will be using this antibody in starfish eggs, I compared the starfish protein sequence to the human protein sequence and found areas of high identity between the two and I want to use an antibody that is expected to bind to amino acids common to both. Therefore, I would like some more information to match the immunogen to the starfish protein. Can you tell me specifically which amino acids of human FLT1 were used to make the antibody?
   • The immunogen sequence to this product is proprietary. I can tell you it falls between amino acids 800-900 of the mouse FLT1 protein [UniProt# P35969].
2. Would you be able to tell me the concentration of the current lot of NB100-527 - VEGFR1/VEGFR2 Ab?
   • The concentration of NB100-527 is 1mg/mL

Bioinformatics

• Gene Symbol: FLT1