Neuropilin-1 Antibody [Unconjugated]

Western blot shows 25 ng of Recombinant Mouse Neuropilin‑1 (Catalog # 5994-N1), Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin‑1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin‑1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Neuropilin‑1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti‑Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neuropilin‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuropilin-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
  Phe22-Ala810 (Lys811Arg), Ser829-Asp854
  Accession # Q9QWJ9
• Specificity: Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRP1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-1 Antibody [Unconjugated]

• BDCA4
• BDCA-4
• CD304 antigen
• CD304
• DKFZp686A03134
• DKFZp781F1414
• neuropilin 1
• Neuropilin1
• Neuropilin-1
• NP1
• NRP
• NRP1
• transmembrane receptor
• Vascular endothelial cell growth factor 165 receptor
• VEGF165R

Background

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuropilin-1 Antibody (AF566)(51)

Publications using AF566

• CM Termini, A Pang, T Fang, M Roos, VY Chang, Y Zhang, NJ Setiawan, L Signaevska, M Li, MM Kim, O Tabibi, PK Lin, JP Sasine, A Chatterjee, R Murali, HA Himburg, JP Chute Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution Nature Communications, 2021-11-30;12(1):6990. 2021-11-30 [PMID: 34848712] (Neutralization, Mouse)
• EMMA Andriessen, F Binet, F Fournier, M Hata, A Dejda, G Mawambo, S Crespo-Gar, F Pilon, M Buscarlet, K Beauchemin, V Bougie, G Cumberlidg, AM Wilson, S Bourgault, FA Rezende, N Beaulieu, JS Delisle, P Sapieha Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation Embo Molecular Medicine, 2021-04-19;0(0):e11754. 2021-04-19 [PMID: 33876574] (IHC, Mouse)
• G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021-01-26;34(4):108644. 2021-01-26 [PMID: 33503438] (Neutralization, Mouse, Transgenic Mouse)
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)
• J Gonzales, C Le Berre-S, A Dariel, P Bréhéret, M Neunlist, H Boudin Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease Sci Rep, 2020-09-15;10(1):15119. 2020-09-15 [PMID: 32934297] (Western Blot, Human, Rat)
• T Pemmari, L Ivanova, U May, P Lingasamy, A Tobi, A Pasternack, S Prince, O Ritvos, S Makkapati, T Teesalu, MS Cairo, TAH Järvinen, Y Liao Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa Mol. Ther., 2020-05-20;0(0):. 2020-05-20 [PMID: 32497513] (IHC, Mouse)
• DM Barry, EA McMillan, B Kunar, R Lis, T Zhang, T Lu, E Daniel, M Yokoyama, JM Gomez-Sali, A Sureshbabu, O Cleaver, A Di Lorenzo, ME Choi, J Xiang, D Redmond, SY Rabbany, T Muthukumar, S Rafii Molecular determinants of nephron vascular specialization in the kidney Nat Commun, 2019-12-13;10(1):5705. 2019-12-13 [PMID: 31836710] (IHC, Mouse)
• EZM Taroc, A Naik, JM Lin, NB Peterson, DL Keefe, E Genis, G Fuchs, R Balasubram, PE Forni Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration J. Neurosci., 2019-11-25;0(0):. 2019-11-25 [PMID: 31767679] (IHC, Mouse)
• MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 2019-08-20;14(8):e0221440. 2019-08-20 [PMID: 31430342] (IHC, Western Blot, Mouse)
• CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 2019-07-12;0(0):. 2019-07-12 [PMID: 31300520] (Cell Culture, Mouse)
• D Nilsson, M Heglind, Z Arani, S Enerbäck Foxc2 is essential for podocyte function Physiol Rep, 2019-05-01;7(9):e14083. 2019-05-01 [PMID: 31062503] (IHC, Mouse)
• AM Wilson, Z Shao, V Grenier, G Mawambo, JF Daudelin, A Dejda, F Pilon, N Popovic, S Boulet, C Parinot, M Oubaha, N Labrecque, V de Guire, M Laplante, G Lettre, F Sennlaub, JS Joyal, M Meunier, P Sapieha Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome Sci Immunol, 2018-03-16;3(21):. 2018-03-16 [PMID: 29549139] (IHC, Mouse)
• H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba Rewiring the taste system Nature, 2017-08-09;548(7667):330-333. 2017-08-09 [PMID: 28792937] (IHC, Mouse)
• K Inokuchi, F Imamura, H Takeuchi, R Kim, H Okuno, H Nishizumi, H Bito, T Kikusui, H Sakano Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses Nat Commun, 2017-07-21;8(0):15977. 2017-07-21 [PMID: 28731029] (IHC-Fr, Mouse)
• JM Pinskey, NE Franks, AN McMellen, RJ Giger, BL Allen Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif J. Biol. Chem., 2017-06-30;0(0):. 2017-06-30 [PMID: 28667171] (ICC, Mouse)
• N Yang, W Zhang, T He, Y Xing Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy J Ophthalmol, 2017-03-24;2017(0):5053035. 2017-03-24 [PMID: 28428895] (IHC-Fr, Mouse)
• N Yang, W Zhang, T He, Y Xing Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 2017-02-28;0(0):. 2017-02-28 [PMID: 28257831] (IHC, Mouse)
• P Fern ndez-, S Selvam, MB Powner, DA Sim, M Fruttiger Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation PLoS ONE, 2017-01-20;12(1):e0169865. 2017-01-20 [PMID: 28107458] (IHC, Mouse)
• Peter Mombaerts Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb eNeuro, 2016-11-01;3(5):. 2016-11-01 [PMID: 27844052] (IHC, Mouse)
• NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 2016-05-25;5(0):. 2016-05-25 [PMID: 27223325] (IHC, Mouse)
• Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 2015-06-08;125(7):2661-76. 2015-06-08 [PMID: 26053665] (IHC, Mouse)
• Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 2015-04-20;21(5):483-91. 2015-04-20 [PMID: 25894826] (IHC, Mouse)
• Tillo M, Erskine L, Cariboni A, Fantin A, Joyce A, Denti L, Ruhrberg C VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain. Development, 2014-12-17;142(2):314-9. 2014-12-17 [PMID: 25519242] (IHC, Mouse)
• Ding X, Lucas T, Marcuzzi G, Pfister H, Eming S Distinct functions of epidermal and myeloid-derived VEGF-A in skin tumorigenesis mediated by HPV8. Cancer Res, 2014-11-20;75(2):330-43. 2014-11-20 [PMID: 25414138] (IHC, Mouse)
• Lee , Mike, Kiefel , Helena, LaJevic , Melissa, Macauley , Matthew, Kawashima , Hiroto, O'Hara , Edward, Pan , Junliang, Paulson , James C, Butcher , Eugene C Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol, 2014-08-31;15(10):982-95. 2014-08-31 [PMID: 25173345] (IHC, Mouse)
• Petzold C, Steinbronn N, Gereke M, Strasser R, Sparwasser T, Bruder D, Geffers R, Schallenberg S, Kretschmer K Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin. Eur J Immunol, 2014-09-23;44(12):3632-45. 2014-09-23 [PMID: 25159127] (Flow Cytometry, Mouse)
• Miyazaki , Masaki, Miyazaki , Kazuko, Chen , Shuwen, Itoi , Manami, Miller , Marina, Lu , Li-Fan, Varki , Nissi, Chang , Aaron N, Broide , David H, Murre , Cornelis Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease. Nat Immunol, 2014-06-29;15(8):767-76. 2014-06-29 [PMID: 24973820] (Flow Cytometry, Mouse)
• Raimondi C, Fantin A, Lampropoulou A, Denti L, Chikh A, Ruhrberg C Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. J Exp Med, 2014-05-26;211(6):1167-83. 2014-05-26 [PMID: 24863063] (Western Blot, Human, Mouse)
• Shahrabi-Farahani , Shokoufe, Wang , Lili, Zwaans , Bernadet, Santana , Jeans M, Shimizu , Akio, Takashima , Seiji, Kreuter , Michael, Coultas , Leigh, D'Amore , Patricia, Arbeit , Jeffrey, Akslen , Lars A, Bielenberg , Diane R Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Lab Invest, 2014-05-05;94(7):752-65. 2014-05-05 [PMID: 24791743] (Western Blot, Mouse)
• Smolarchuk C, Zhu L, Chan W, Anderson C T cells generated in the absence of a thoracic thymus fail to establish homeostasis. Eur J Immunol, 2014-05-23;44(8):2263-73. 2014-05-23 [PMID: 24777700] (Flow Cytometry, Mouse)
• Molofsky A, Kelley K, Tsai H, Redmond S, Chang S, Madireddy L, Chan J, Baranzini S, Ullian E, Rowitch D Astrocyte-encoded positional cues maintain sensorimotor circuit integrity. Nature, 2014-04-28;509(7499):189-94. 2014-04-28 [PMID: 24776795] (Neutralization, Rat)
• Esquibies A, Karihaloo A, Quaggin S, Bazzy-Asaad A, Cantley L Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway. Respir Res, 2014-03-19;15(0):32. 2014-03-19 [PMID: 24641672] (Functional Assay, Mouse)
• Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol, 2014-03-11;12(3):e1001808. 2014-03-11 [PMID: 24618750] (IHC, Immunoprecipitation, Neutralization, Western Blot, Mouse, Rat)
• Fantin , Alessand, Herzog , Birger, Mahmoud , Marwa, Yamaji , Maiko, Plein , Alice, Denti , Laura, Ruhrberg , Christia, Zachary , Ian Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis. Development, 2014-01-08;141(3):556-62. 2014-01-08 [PMID: 24401374] (Western Blot, Mouse)
• Ezkurdia N, Raurell I, Rodriguez S, Gonzalez A, Esteban R, Genesca J, Martell M Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. PLoS ONE, 2014-01-06;9(1):e84374. 2014-01-06 [PMID: 24400086] (IHC-P, Rat)
• Delgoffe , Greg M, Woo , Seng-Ryo, Turnis , Meghan E, Gravano , David M, Guy , Cliff, Overacre , Abigail, Bettini , Matthew, Vogel , Peter, Finkelstein , David, Bonnevier , Jody, Workman , Creg J, Vignali , Dario A Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature, 2013-08-04;501(7466):252-6. 2013-08-04 [PMID: 23913274] (Immunoprecipitation, Mouse)
• van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9. 2013-02-21 [PMID: 23430612] (Neutralization, Human)
• Fantin A, Vieira J, Plein A, Maden C, Ruhrberg C The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis. Nat Protoc, 2013-02-01;8(2):418-29. 2013-02-01 [PMID: 23424750] (IHC, Mouse)
• Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J. Exp. Med., 2012-10-08;209(11):2001-16. 2012-10-08 [PMID: 23045606] (Flow Cytometry, IHC-Fr, Mouse)
• Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells. J. Exp. Med., 2012-09-10;209(10):1723. 2012-09-10 [PMID: 22966001] (Flow Cytometry, IHC-Fr, Mouse)
• Glinka Y, Mohammed N, Subramaniam V, Jothy S Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-kappaB activation and tumor sphere formation. Biochem. Biophys. Res. Commun., 2012-08-02;425(4):775-80. 2012-08-02 [PMID: 22885184] (Flow Cytometry, Human)
• Chakraborty G, Kumar S, Mishra R Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. PLoS ONE, 2012-03-20;7(3):e33633. 2012-03-20 [PMID: 22448259] (Neutralization, Human, Mouse)
• Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite-de-Moraes M, Rubio MT, Hermine O IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression. Blood, 2011-06-08;118(11):2993-3002. 2011-06-08 [PMID: 21653940] (Flow Cytometry, Mouse)
• Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 2010-04-01;120(5):1694-707. 2010-04-01 [PMID: 20364082] (IHC-P, Mouse)
• Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis. J. Biol. Chem., 2009-10-16;284(49):33966-81. 2009-10-16 [PMID: 19837659] (Western Blot, Mouse)
• Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 2008-06-01;118(6):2062-75. 2008-06-01 [PMID: 18483621] (IHC-Fr, Mouse)
• Marko SB, Damon DH VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 2008-04-11;294(6):H2646-52. 2008-04-11 [PMID: 18408130] (ICC, IHC-Fr, Western Blot, Rat)
• Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y BIG-2 mediates olfactory axon convergence to target glomeruli. Neuron, 2008-03-27;57(6):834-46. 2008-03-27 [PMID: 18367085] (IHC, Western Blot, Mouse)
• Chauvet S, Cohen S, Yoshida Y, Fekrane L, Livet J, Gayet O, Segu L, Buhot MC, Jessell TM, Henderson CE, Mann F Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development. Neuron, 2007-12-06;56(5):807-22. 2007-12-06 [PMID: 18054858] (ICC, Mouse)
• Moret F, Renaudot C, Bozon M, Castellani V Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding. Development, 2007-12-01;134(24):4491-501. 2007-12-01 [PMID: 18039974] (IHC-Fr, Chicken)
• Mire E, Thomasset N, Jakeman LB, Rougon G Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury. Mol. Cell. Neurosci., 2007-10-03;37(2):222-35. 2007-10-03 [PMID: 17997325] (IHC, Mouse)

Reviews for Neuropilin-1 Antibody (AF566) (10)

Average Rating: 4.2

(Based on 10 reviews)

We have 10 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Anonymous - IF Other 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22927827
• Species: Other

reviewed by: Anonymous - IHC-Fr Mouse 01/08/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 24034253
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23406903
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24021649
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23602477
• Species: Mouse

reviewed by: cynthia morimoto IF Mouse 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Primary mouse hippocampal neurons
• Species: Mouse

reviewed by: cynthia morimoto IF Mouse 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Hippocampal neurons and astrocytes
• Species: Mouse

reviewed by: cynthia morimoto WB Rat 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: RN22 cells
• Species: Rat

reviewed by: cynthia morimoto WB Mouse 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Mouse primary cultures
• Species: Mouse

reviewed by: cynthia morimoto WB Human 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: a number of human cell lines
• Species: Human

FAQs for Neuropilin-1 Antibody (AF566). (Showing 1 - 2 of 2 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
2. What research areas can Neuropilin-1 antibodies be used in?
   • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Bioinformatics

• Gene Symbol: NRP1
• Uniprot:
  • Q9QWJ9()