VEGF Antibody (VG1)


Western Blot: VEGF Antibody (VG1) [NB100-664] - HUVEC and 293T Cells tested at 1:1000. Image provided by verified customer review.
Immunocytochemistry/ Immunofluorescence: VEGF Antibody (VG1) [NB100-664] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were more
Immunohistochemistry-Paraffin: VEGF Antibody (VG1) [NB100-664] - Analysis of Vascular Endothelial GF formalin-fixed paraffin embedded human angiosarcoma.
Western Blot: VEGF Antibody (VG1) [NB100-664] - Western blot analysis of VEGF in human kidney protein using NB100-664.

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

VEGF Antibody (VG1) Summary

Recombinant VEGF 189 protein.
This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:20-1:100
  • Immunohistochemistry-Frozen 1:20-1:100
  • Immunohistochemistry-Paraffin 1:20-1:100
Application Notes
This VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.
Reviewed Applications
Read 1 Review rated 5
NB100-664 in the following applications:

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NB100-664 in the following applications:

Reactivity Notes

Human, Mouse, Rat, and Dog.

Alternate Names for VEGF Antibody (VG1)

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IP
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Publications for VEGF Antibody (NB100-664)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB100-664 Applications Species
Su JC, Mar AC, Wu SH et al. Disrupting VEGF-A paracrine and autocrine loops by targeting SHP-1 suppresses triple negative breast cancer metastasis Sci Rep 2016 Jul 01 [PMID:27364975] (IHC) IHC
Chen SW, Lin YC, Chen RY et al. Immunohistochemical overexpression of hypoxia-induced factor 1a associated with slow reduction in (18)fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer. Eur. J. Nucl. Med. Mol. Imaging Jun 16 2016 12:00AM [PMID:27311919] (IHC) IHC
Ram M, Singh V, Kumawat S et al. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int. Immunopharmacol. 2016 Jan 01 [PMID:26679676] (WB, Rat) WB Rat
Srinivas C, Ramaiah MJ, Lavanya A et al. Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer. PLoS ONE. 2015 Nov 10 [PMID:26551008] (WB, Human) WB Human
Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015 Oct [PMID:26501235] (WB, Mouse) WB Mouse
Ram M, Singh V, Kumawat S et al. Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats. Eur J Pharmacol 2015 Oct 5 [PMID:26101070] (Rat) Rat
Shao J, Zhang Y, Yang T et al. HIF-1alpha disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression In Vitro Cell. Dev. Biol. Anim. 2015 Apr 10 [PMID:25859979] (WB, Mouse) WB Mouse
Tam JCW, Ko CH, Lau KM et al. A Chinese 2-herb formula (NF3) promotes hindlimb ischemia-induced neovascularization and wound healing in diabetic rats. Journal of Diabetes and … 3/21/2014 [PMID:24731763] (IHC-Fr, Rat) IHC-Fr Rat
Parlakgumus H Ayse, Aka Bolat Filiz, Bulgan Kilicdag Esra et al. Atorvastatin for ovarian torsion: effects on follicle counts, AMH, and VEGF expression. Eur J Obstet Gynecol Reprod Biol. 2014 Jan 20 [PMID:24507756] (Rat) Rat
Johansen FF, Hasseldam H, Rasmussen RS et al. Drug-Induced Hypothermia as Beneficial Treatment before and after Cerebral Ischemia. Pathobiology. 2013 Aug 23 [PMID:23989388]
Show All 30 Publications.

Reviews for VEGF Antibody (NB100-664) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-664:
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Images Ratings Applications Species Date Details
Western Blot VEGF NB100-664
reviewed by:
WB Human 02/28/2014


ApplicationWestern Blot
Sample TestedHUVEC and 293T cells


Blocking Details100mL Tris buffered saline, 5g BSA, 0.1mL Tween 20. Incubate overnight at 4C

Primary Anitbody

Dilution RatioDilute 1:1000

Secondary Antibody

Secondary DescriptionChicken Anti-mouse HRP
Secondary Manufacturer Cat#NBP1-75102
Secondary Concentration1:5000


Detection NotesAmersham ECL, Hyperfilm ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGF Antibody (NB100-664). (Showing 1 - 6 of 6 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. I would be very grateful if you could let me know a recommended dilution for the VEGF antibody antibody NB100-664
    • The recommended dilution for IHC is 1:20-1:50 in an ABC method. If instead you are testing on cell culture, I would recommend a starting optimization point of 1:10 -1:2000.
  3. I would like to know the aproximate dilution of the VEGF Antibody (NB100-664) to use it in a Western blot assay.
    • In regards to your inquiry about our VEGF antibody (NB100-664), I am showing from the lab data that they have tested dilutions in the range of 1:500-1:1000 for this antibody. The optimal dilution should depend on your samples and the level of expression of VEGF, but a good starting point would be 1:1000 and then optimize from there.
  4. What is the minimum amount of protein necessary for the detection of VEGF by Western blot using VEGF Antibody (NB100-664)?
    • I would suggest at minimum 10ug of protein per well but if you have enough sample you might want to run multiple wells with increasing amounts of protein to optimize the conditions in your hands. You could start with 5ug and do a couple of wells with 10ug, 20ug and 40ug. Also try using different dilutions of primary, start with 1:500-1:1000 and work your way up or down depending on what your blot looks like.
  5. I am interested in buying an antibody to VEGF to detect VEGF in human umbilical vein endothelial cells for western blotting and was wondering if you might have any recommendations? I was thinking about NB100-664. Do you have a recommended amount to use for western blotting? Could I ask you what concentration the antibody is at since it says 0.1mg so what total volume do you send? Also is this the most commonly purchase one for western blotting do you know?
    • We do not have a recommended dilution noted for this specific antibody, but our general recommendation for Western blot is 1:1000-1:2000.This is a good starting point, but we always recommend optimization by the end user, as the optimal dilution is to some extent dependent on the particular sample/assay. The concentration for this product is stated as 1 mg/ml, so 0.1 mg would be supplied in 0.1 ml.
  6. I am looking for a VEGF-A antibody for the IHC staining of mouse colon. Do you have any publications or application references for this product?
    • NB100-664 is one of our best sellers with great customer feedback and citations in at least 18 peer reviewed publications from highly esteemed researchers. As far as mouse tissues are concerned, I would recommend that you go through the following paper: Milanesi A, et al. Beta-Cell regeneration mediated by human bone marrow mesenchymal stem cells. PLoS One. 2012;7(8):e42177. doi: 10.1371/journal.pone.0042177. Epub 2012 Aug 7. PMID:22879915.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-664AF405
Alexa Fluor (R) 488 Labeled NB100-664AF488
Alexa Fluor (R) 647 Labeled NB100-664AF647
Alexa Fluor (R) 700 Labeled NB100-664AF700
Allophycocyanin Labeled NB100-664APC
Biotin Labeled NB100-664B
DyLight 350 Labeled NB100-664UV
DyLight 405 Labeled NB100-664V
DyLight 405LS Labeled NB100-664V3
DyLight 488 Labeled NB100-664G
DyLight 550 Labeled NB100-664R
DyLight 650 Labeled NB100-664C
DyLight 680 Labeled NB100-664FR
DyLight 755 Labeled NB100-664IR
FITC Labeled NB100-664F
HRP Labeled NB100-664H
PE Labeled NB100-664PE
PerCP Labeled NB100-664PCP

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Blogs on VEGF. Showing 1-10 of 12 blog posts - Show all blog posts.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

LYVE1 - It's Alive! It's a LYVE
LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass t...  Read full blog post.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

VEGF Receptors, Angiogenesis and Cancer
Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Gene Symbol VEGFA

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