VCAM-1/CD106 Antibody (SA05-04)

Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of VCAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: human spleen tissue lysate

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Staining VCAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded mouse spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC, IP
• Clone: SA05-04
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

VCAM-1/CD106 Antibody (SA05-04) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human VCAM-1/CD106 aa 690-739 / 739. (SwissProt: P19320 Human; SwissProt: P29533 Mouse; SwissProt: P29534 Rat)
• Localization: Membrane
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:5000
• Application Notes: IP - assay-dependent

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VCAM-1/CD106 Antibody (SA05-04)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP2-67292)(1)

Publication using NBP2-67292

• Buijsers B, Maciej-Hulme M, Jacobs M et al. Glycosaminoglycans and fucoidan have a protective effect on experimental glomerulonephritis Frontiers in molecular biosciences 2023-07-05 [PMID: 37475889] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: VCAM1