VCAM-1/CD106 Antibody (6G9)

Images

 
Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - using VCAM1 mouse mAb against HUVEC (1) and EC (2) cell lysate.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. Image from verified customer review.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - human lung cancer (A) and colon cancer (B) using VCAM1 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - human Placenta tissues using VCAM1 mouse mAb
Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Simple Western lane view shows a specific band for CD106/VCAM1 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC, IHC-P
Clone
6G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

VCAM-1/CD106 Antibody (6G9) Summary

Immunogen
Purified recombinant fragment of human VCAM1 expressed in E. coli. [UniProt# P19320]
Localization
Membrane; Single-pass type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VCAM1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:1000
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This VCAM1 (6G9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47491 in the following applications:

Publications
Read Publications using
NBP1-47491 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for VCAM-1/CD106 Antibody (6G9)

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VCAM-1/CD106 Antibody (NBP1-47491)(3)

Review for VCAM-1/CD106 Antibody (NBP1-47491) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC.

Reviews using NBP1-47491:
Filter by Applications
IHC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry VCAM-1/CD106 NBP1-47491
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reviewed by:
Anony- mous
IHC Human 04/21/2014
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Summary

ApplicationImmunohistochemistry
SpeciesHuman
FileView PDF

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Video Protocols

WB Video Protocol

FAQs for VCAM-1/CD106 Antibody (NBP1-47491) (0)

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Bioinformatics Tool for VCAM-1/CD106 Antibody (NBP1-47491)

Discover related pathways, diseases and genes to VCAM-1/CD106 Antibody (NBP1-47491). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for VCAM-1/CD106 Antibody (NBP1-47491)

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PTMs for VCAM-1/CD106 Antibody (NBP1-47491)

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Recent Reviews

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5
1
4
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2
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1
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Anony- mous
04/21/2014
Application: IHC
Species: Human

Bioinformatics

Gene Symbol VCAM1
Entrez
Uniprot