UCH-L1/PGP9.5 Antibody

Images

 
Genetic Strategies: Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF ...read more
Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB300-676] - Staining of neurons and cell processes at 2.5ug/mL. Human brain, Substantia Nigra, Pigmented Neurons, 4X.
Flow Cytometry: UCH-L1/PGP9.5 Antibody [NB300-676] - An intracellular stain was performed on U-87 cells with UCH-L1/PGP9.5 Antibody NB300-676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Simple Western: UCH-L1/PGP9.5 Antibody [NB300-676] - Image shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Eq, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

UCH-L1/PGP9.5 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human UCHL1 protein (between residues 200-220). [UniProt# P09936]
Localization
Cytoplasmic
Marker
pan-Neuronal Marker
Predicted Species
Rat (100%), Porcine (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2-5 ug/million cells/ml
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 2.5 - 5.0 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 - 5.0 ug/mL
  • Knockout Validated
  • Simple Western 1:2000
  • Western Blot 2 ug/mL
Application Notes
By Western blot a band at ~25 kDa is seen. Use in IHC-Frozen reported in scientific literature (PMID 25041530)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
UCH-L1/PGP9.5 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB300-676 in the following applications:

Publications
Read Publications using
NB300-676 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCH-L1/PGP9.5 Antibody

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-676
Species: Hu, Mu, Rt, Po, Eq, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO

Publications for UCH-L1/PGP9.5 Antibody (NB300-676)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-Fr, WB.


Filter By Application
IHC-Fr
(1)
WB
(2)
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Human
(2)
Mouse
(1)
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Review for UCH-L1/PGP9.5 Antibody (NB300-676) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-676:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen UCH-L1/PGP9.5 NB300-676
Enlarge
3
reviewed by:
Brian McAdams
IHC-Fr Human 11/25/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedhuman skin, 60micron thick section for confocal immunofluorescent imaging
SpeciesHuman
LotA-1

Product General Protocols

View specific protocols for UCH-L1/PGP9.5 Antibody (NB300-676): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCH-L1/PGP9.5 Antibody (NB300-676) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
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3
1
2
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1
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Brian McAdams
11/25/2015
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol UCHL1