EN-RAGE/S100A12 Antibody [Unconjugated]

Images

 
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated ...read more
EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

EN-RAGE/S100A12 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EN-RAGE/S100A12
Met1-Glu92
Accession # P80511
Specificity
Detects human EN-RAGE in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100A12
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 3 Reviews rated 4
using
AF1052 in the following applications:

Publications
Read Publications using
AF1052 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EN-RAGE/S100A12 Antibody [Unconjugated]

  • CAAF1
  • CAAF1Neutrophil S100 protein
  • CAAFI
  • CAGC
  • CAGCS100 calcium binding protein A12 (calgranulin C)
  • CAGCS100
  • Calcium-binding protein in amniotic fluid 1
  • Calgranulin C
  • calgranulin-C
  • CGRPEN-RAGE
  • ENRAGE
  • EN-RAGE
  • Extracellular newly identified RAGE-binding protein
  • MRP6
  • p6calgranulin C
  • protein S100-A12
  • S100 calcium binding protein A12
  • S100 calcium-binding protein A12 (calgranulin C)
  • S100 calcium-binding protein A12
  • S100A12

Background

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EN-RAGE/S100A12 Antibody (AF1052)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC Paraffin-embedded, WB.


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IHC Paraffin-embedded
(1)
WB
(1)
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Filter By Species
Human
(2)
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Reviews for EN-RAGE/S100A12 Antibody (AF1052) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using AF1052:
Filter by Applications
WB
(1)
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19944411
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19944411

Details

Detection NotesSee PMID 19944411

Comments

CommentsPublished PMID 19944411
 
reviewed by:
Anonymous
IHC-P Human 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22609404
SpeciesHuman
 
reviewed by:
Anonymous
IHC-P Other 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22609404
SpeciesOther

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Secondary Antibodies

 

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Bioinformatics Tool for EN-RAGE/S100A12 Antibody (AF1052)

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: WB
Species: Human

 
Anonymous
01/05/2015
Application: IHC-P
Species: Human

 
Anonymous
01/05/2015
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol S100A12
Entrez
Uniprot