EN-RAGE/S100A12 Antibody [Unconjugated]

Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human tonsil, loaded at 0.2 mg/mL. A specific band was detected for EN‑RAGE/S100A12 at approximately 11 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EN-RAGE/S100A12 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human EN-RAGE/S100A12
  Met1-Glu92
  Accession # P80511
• Specificity: Detects human EN-RAGE in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EN-RAGE/S100A12 Antibody [Unconjugated]

• CAAF1
• CAAF1Neutrophil S100 protein
• CAAFI
• CAGC
• CAGCS100 calcium binding protein A12 (calgranulin C)
• CAGCS100
• Calcium-binding protein in amniotic fluid 1
• Calgranulin C
• calgranulin-C
• CGRPEN-RAGE
• ENRAGE
• EN-RAGE
• Extracellular newly identified RAGE-binding protein
• MRP6
• p6calgranulin C
• protein S100-A12
• S100 calcium binding protein A12
• S100 calcium-binding protein A12 (calgranulin C)
• S100 calcium-binding protein A12
• S100A12

Background

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
2. Leclerc, E. et al. (2009) Biochim. Biophys. Acta 1793:993.
3. Wicki, R. et al. (1996) Cell Calcium 20:459.
4. Moroz, O.V. et al. (2009) BMC Biochem. 10:11.
5. Miranda, L.P. et al. (2001) FEBS Lett. 488:85.
6. Vogl, T. et al. (1999) J. Biol. Chem. 274:25291.
7. Xie, J. et al. (2007) J. Biol. Chem. 282:4218.
8. Goyette, J. et al. (2009) J. Immunol. 183:593.
9. Hatakeyama, T. et al. (2004) Eur. J. Biochem. 271:3765.
10. Hofmann, M.A. et al. (1999) Cell 97:889.
11. Yang, Z. et al. (2001) J. Leukoc. Biol. 69:986.
12. Rouleau, P. et al. (2003) Clin. Immunol. 107:46.
13. Yan, W.X. et al. (2008) J. Biol. Chem. 283:13035.
14. Yang, Z. et al. (2007) J. Allergy Clin. Immunol. 119:106.
15. Mikkelsen, S.E. et al. (2001) J. Neurochem. 79:767.
16. Fuellen, G. et al. (2004) OMICS 8:334.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EN-RAGE/S100A12 Antibody (AF1052)(2)

Publications using AF1052

• S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin. Cancer Res., 2012;18(16):4356-64. 2012 [PMID: 22718861] (IHC-P, Human)
• Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells. Fertil. Steril., 2010;94(5):1639. 2010 [PMID: 19944411] (Western Blot, Human)

Reviews for EN-RAGE/S100A12 Antibody (AF1052) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Other.

reviewed by: Anonymous - WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19944411
• Species: Human

reviewed by: Anonymous - IHC-P Human 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22609404
• Species: Human

reviewed by: Anonymous - IHC-P Other 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22609404
• Species: Other

Bioinformatics

• Gene Symbol: S100A12
• Uniprot:
  • P80511()