alpha-Synuclein Antibody

Images

 
Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's ...read more
Immunocytochemistry/ Immunofluorescence: alpha-Synuclein Antibody [NBP2-15365] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by ...read more
Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by ...read more
Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:2000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded Rat brain diluted at 1:500.
Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded rat brain. Green: alpha Synuclein antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody ...read more
Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen sectioned E13.5 Rat brain. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: beta Tubulin 3/ ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

alpha-Synuclein Antibody Summary

Immunogen
Full length human alpha Synuclein Recombinant protein.
Localization
Cytoplasm; Membrane; Nucleus
Predicted Species
Porcine (97%), Bovine (94%), Primate (100%), Sheep (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNCA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15365 in the following applications:

Publications
Read Publications using
NBP2-15365 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Chimpanzee (100%), Chicken (88%), Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for alpha-Synuclein Antibody

  • alpha-Synuclein
  • Lewy body) 4
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • non-A4 component of amyloid
  • PARK1
  • PARK4
  • SNCA
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Synuclein Antibody (NBP2-15365)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP2-15365 Applications Species
Zhu J, Dou S, Wang C et al. Apelin-36 mitigates MPTP/MPP+-induced neurotoxicity: involvement of alpha-synuclein and endoplasmic reticulum stress Brain Res. Jul 12 2019 [PMID: 31306618] (ICC/IF, WB, Mouse, Human) ICC/IF, WB Mouse, Human
Akerman, SC;Hossain, S;Shobo, A;Zhong, Y;Jourdain, R;Hancock, MA;George, K;Breton, L;Multhaup, G; Neurodegenerative Disease-Related Proteins within the Epidermal Layer of the Human Skin J. Alzheimers Dis. Apr 15 2019 [PMID: 31006686] (ICC/IF, Human) ICC/IF Human
Plaksina D, Ekimova I Age-Related Features of alpha-Synuclein Pathology in the Brain on Modeling the Preclinical Stage of Parkinson's Disease in Rats Neurosci Behav Physi Jan 1 2020 (IHC) IHC

Review for alpha-Synuclein Antibody (NBP2-15365) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse and Human.

Reviews using NBP2-15365:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot alpha-Synuclein NBP2-15365
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reviewed by:
Anonymous
WB Mouse and Human 12/18/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse forebrain lysate
SpeciesMouse and Human
Lot42837

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Synuclein Antibody (NBP2-15365). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Secondary Antibodies

 

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Discover related pathways, diseases and genes to alpha-Synuclein Antibody (NBP2-15365). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on alpha-Synuclein.

Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

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Recent Reviews

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1
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Anonymous
12/18/2017
Application: WB
Species: Mouse and Human

Bioinformatics

Gene Symbol SNCA
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