Park7/DJ-1 Antibody

Images

 
Western Blot: Park7/DJ-1 Antibody [NB300-270] - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) ...read more
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in paraffin embedded human cortex.
Western Blot: Park7/DJ-1 Antibody [NB300-270] - Human HEK293 cells and mouse brain lysates.
Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Bv, Ch, SyHa, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P, In vitro, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Park7/DJ-1 Antibody Summary

Immunogen
A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]
Localization
Nucleus. Cytoplasm. Associated with mitochondria in some cells, particularly after oxidative stress. Detected in tau inclusions in brains from neurodegenerative disease patients.
Predicted Species
Chicken (100%), Golden Syrian Hamster (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARK7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:4000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:200
  • In vitro assay
Application Notes
This Park7(DJ-1) antibody may be used for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, In vitro assay (PMID: 21645620) and Western Blot, where a band can be seen at approx. 25 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Park7/DJ-1 Lysate (NBL1-14106)
Park7/DJ-1 Lysate (NBP2-65683)
Reviewed Applications
Read 2 Reviews rated 5
using
NB300-270 in the following applications:

Publications
Read Publications using
NB300-270 in the following applications:

  • 1 publication
  • 3 publications
  • IP
    4 publications
  • WB
    6 publications

Reactivity Notes

Human and mouse. Predicted to react with rat, bovine, Zebra fish, Golden Syrian hamster, and chicken based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392). Rat reactivity reported in scientific literature (PMID: 26419955)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

Park7/DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinsonism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-270
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Publications for Park7/DJ-1 Antibody (NB300-270)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 4 applications: FLOW, ICC/IF, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(3)
IP
(4)
WB
(6)
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Filter By Species
Human
(6)
Mouse
(7)
Chicken
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB300-270 Applications Species
Porrini V, Mota M, Parrella E et al. Mild Inflammatory Profile without Gliosis in the c-Rel Deficient Mouse Modeling a Late-Onset Parkinsonism. Front Aging Neurosci 2017 Aug 03 [PMID: 28769786] (ICC/IF, Mouse) ICC/IF Mouse
Bonilha VL, Bell BA, Rayborn ME et al. Loss of DJ-1 elicits retinal abnormalities, visual dysfunction, and increased oxidative stress in mice Exp. Eye Res. 2015 Jul 26 [PMID: 26215528] (WB, Mouse) WB Mouse
Deng J, Zhao F, Yu X et al. Identification of the Protective Role of DJ-1 in Hypoglycemic Astrocyte Injury Using Proteomics. J Proteome Res 2015 Jun 19 [PMID: 26057206]
Choi MS, Nakamura T, Cho SJ et al. Transnitrosylation from DJ-1 to PTEN attenuates neuronal cell death in parkinson's disease models. J Neurosci 2014 Nov 5 [PMID: 25378175] (Human) Human
Minakawa EN, Yamakado H, Tanaka A et al. Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction. Neurosci Res. 2013 Sep 21 [PMID: 24064392] (WB, Chicken) WB Chicken
Shadrach KG, Rayborn ME, Hollyfield JG, Bonilha VL. DJ-1-Dependent Regulation of Oxidative Stress in the Retinal Pigment Epithelium (RPE). PLoS One 2013 Jul 2 [PMID: 23844142] (ICC/IF, WB, Mouse, Human) ICC/IF, WB Mouse, Human
Yasuda T, Kaji Y, Agatsuma T et al. DJ-1 cooperates with PYCR1 in cell protection against oxidative stress. Biochem Biophys Res Commun 2013 Jun 3 [PMID: 23743200] (IP, Human) IP Human
Lin X, Cook TJ, Zabetian CP et al. DJ-1 isoforms in whole blood as potential biomarkers of Parkinson disease Sci Rep 2012 [PMID: 23233873] (IP, WB, Human) IP, WB Human
Heo JY, Park JH, Kim SJ, Seo KS, Han JS et al. DJ-1 Null Dopaminergic Neuronal Cells Exhibit Defects in Mitochondrial Function and Structure: Involvement of Mitochondrial Complex I Assembly PLoS ONE 2012 [PMID: 22403686] (WB, Mouse) WB Mouse
Cuevas S, Zhang Y, Yang Y, Escano C, Asico L, Jones JE, Armando I, Jose PA. Role of renal DJ-1 in the pathogenesis of hypertension associated with increased reactive oxygen species production. Hypertension;59(2):446-52. 2012 Feb. [PMID: 22215708]
Show All 15 Publications.

Reviews for Park7/DJ-1 Antibody (NB300-270) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB300-270:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Park7/DJ-1 NB300-270
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reviewed by:
Anonymous
WB Human 09/10/2013
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsThe antibody also works well for immunofluorescence of paraformaldehyde fixed cell cultures but I did not try it with tissues. Check the attached PDF for image descriptions and protocol details.
Pub Med ID23844142
FileView PDF
Western Blot Park7/DJ-1 NB300-270
Enlarge
reviewed by:
Anonymous
WB Human 12/19/2011
View

Summary

ApplicationWestern Blot
Sample TestedHeLa whole cell lysate, mouse brain
SpeciesHuman
CommentsThis antibody is able to detect endogenous DJ-1 of mouse brain and human cultured cells. This antibody is available for western blotting and immunocytochemistry.
Pub Med ID21645620
FileView PDF

Comments

CommentsThis antibody is able to detect endogenous DJ-1 of mouse brain and human cultured cells. This antibody is available for western blotting and immunocytochemistry.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Park7/DJ-1 Antibody (NB300-270) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics Tool for Park7/DJ-1 Antibody (NB300-270)

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Diseases for Park7/DJ-1 Antibody (NB300-270)

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PTMs for Park7/DJ-1 Antibody (NB300-270)

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Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Recent Reviews

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5
2
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2
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1
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Anonymous
09/10/2013
Application: WB
Species: Human

Anonymous
12/19/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PARK7
Entrez
Uniprot