UCH-L1/PGP9.5 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, GP, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

UCH-L1/PGP9.5 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal portion of the human UCHL1 protein (between residues 100-200). [UniProt# P09936]
Localization
Cytoplasmic
Marker
pan-Neuronal Marker
Predicted Species
Rat (99%), Porcine (99%), Equine (99%), Guinea Pig (100%), Primate (100%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot and Immunohistochemistry-Paraffin. By Western blot a band at ~25 kDa is seen.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Human and mouse. Predicted to react with primate and guinea pig based on 100% sequence homology. Immunogen sequence has 99% homology to rat, pig, and horse, and 92% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for UCH-L1/PGP9.5 Antibody [Biotin]

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UCHL1 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. UCHL1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as PGP9.5. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB300-675G
DyLight 550 Labeled NB300-675R
DyLight 650 Labeled NB300-675C
HRP Labeled NB300-675H

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Bioinformatics

Gene Symbol UCHL1
Entrez