Tubulin Antibody (YL1/2)

Images

 
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - WB analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as ...read more
Immunohistochemistry-Paraffin: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of Tubulin expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Dr, Ma, Pm, Ye
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clone
YL1/2
Clonality
Monoclonal
Host
Rat
Concentration
1.0 mg/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris-glycine, 150 mM NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Tubulin Antibody (YL1/2) Summary

Immunogen
Full length native protein (purified) (S. cerevisiae).
Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Modification
No Modifications
Marker
Microtubule Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • ELISA 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-506 in the following applications:

Publications
Read Publications using
NB600-506 in the following applications:

  • 4 publications
  • WB
    9 publications

Reactivity Notes

Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime moulds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759) Other species have not been tested.

Alternate Names for Tubulin Antibody (YL1/2)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin alpha-1A chain (TUBA1A), also known as alpha-tubulin 3, tubulin B-alpha-1, B-ALPHA-1, tubulin alpha-3 chain, TUBA3, and LIS3, is a member of the tubulin family. Tubulin is the primary constituent of microtubules. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. The TUBA1A gene is one of three alpha tubulin genes located on chromosome 12q. TUBA1A is found on the cytoskeleton and is expressed predominantly in morphologically differentiated neurologic cells. Mutations to the TUBA1A gene cause a neurological condition called lissencephaly type 3 (LIS3). As a result of defective neuronal migration, LIS3 is characterized by microcephaly, mental retardation, and early-onset epilepsy (PMID: 22264709). The tubulin clone (YL1/2) specifically binds tyrosylated Tubulin (Tyr-Tubulin) (PMID: 6415068).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-506
Species: Hu, Mu, Rt, Av, Dr, Ma, Pm, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA

Publications for Tubulin Antibody (NB600-506)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(4)
WB
(9)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
Rat
(4)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB600-506 Applications Species
Hu S, Lu Y, Orr B et al. Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Mol. Cancer Ther. 2015 Aug 24 [PMID:26304236]
Giron-Martinez A, Perez-Rial S, Terron-Exposito R et al. Proliferative Activity of Liver Growth Factor is Associated with an Improvement of Cigarette Smoke-Induced Emphysema in Mice. PLoS OnE. 2014 Nov 18 [PMID:25401951] (WB, Mouse) WB Mouse
Ganem NJ, Cornils H, Chiu SY et al. Cytokinesis failure triggers hippo tumor suppressor pathway activation. Cell. 2014 Aug 14 [PMID:25126788] (WB, Human) WB Human
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013 Sep [PMID:24019759] (WB, Drosophila) WB Drosophila
Sung M, Giannakakou P. BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling Oncogene 2013 Mar 25 [PMID:23524581] (ICC/IF, WB, Human) ICC/IF, WB Human
Batista-Nascimento L, Toledano MB, Thiele DJ et al. Yeast protective response to arsenate involves the repression of the high affinity iron uptake system Biochim Biophys Acta 2013 Jan 4 [PMID:23295455] (WB, Mouse, Human) WB Mouse, Human
O'Connell CB, Loncarek J, Kalab P et al. Relative contributions of chromatin and kinetochores to mitotic spindle assembly. J Cell Biol;187(1):43-51. 2009 [PMID:19805628] (ICC/IF, Rat) ICC/IF Rat
Yang Z, Kenny AE, Brito DA, Rieder CL. Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments. J Cell Biol 2009 Sep 7 [PMID:19720871] (ICC/IF, Human) ICC/IF Human
Ganem NJ et al. A mechanism linking extra centrosomes to chromosomal instability. Nature;460(7252):278-82. 2009 Jul 9. [PMID:19506557] (Rat) Rat
Bhattacharya R, Gonzalez AM, DeBiase PJ et al. Recruitment of vimentin to the cell surface by {beta}3 integrin plectin mediates adhesion strength. J Cell Sci;122(9):1390-1400. 2009 [PMID:19366731] (WB, Rat) WB Rat
Show All 14 Publications.

Reviews for Tubulin Antibody (NB600-506) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 3 application: ICC, WB, IF.

Reviews using NB600-506:
Filter by Applications
ICC
(1)
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Tubulin NB600-506
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reviewed by:
Anonymous
ICC Human 06/11/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot Tubulin NB600-506
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reviewed by:
Anonymous
WB Human 06/11/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS and NIH/3T3 cells using Tubulin antibody (NB600-506, 1:100).
FileView PDF
Immunofluorescence Tubulin NB600-506
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reviewed by:
Anonymous
IF 04/28/2014
View

Summary

ApplicationImmunofluorescence

Product General Protocols

View specific protocols for Tubulin Antibody (NB600-506): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB600-506). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-506AF405
Alexa Fluor (R) 488 Labeled NB600-506AF488
Alexa Fluor (R) 647 Labeled NB600-506AF647
Alexa Fluor (R) 700 Labeled NB600-506AF700
Allophycocyanin Labeled NB600-506APC
DyLight 350 Labeled NB600-506UV
DyLight 405 Labeled NB600-506V
DyLight 405LS Labeled NB600-506V3
DyLight 488 Labeled NB600-506G
DyLight 680 Labeled NB600-506FR
DyLight 755 Labeled NB600-506IR
FITC Labeled NB600-506F
PE Labeled NB600-506PE
PerCP Labeled NB600-506PCP

Additional Tubulin Antibody (YL1/2) Products

Tubulin NB600-506

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TUBA1A

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