alpha Tubulin Antibody (YL1/2) - BSA Free

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - NIH3T3 cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Rat FR cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (NB600-506) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa.

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.

Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY licence.

Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.

Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Rt, Av, Ce, Dr, In, Ma, Pm, Pm, Ye
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
• Clone: YL1/2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

alpha Tubulin Antibody (YL1/2) - BSA Free Summary

• Immunogen: This alpha Tubulin Antibody (YL1/2) was developed against full length native protein (purified) (S. cerevisiae).
• Epitope: A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
• Marker: Microtubule Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TUBA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:1000
  • Flow Cytometry 2-5 ug/0.1x10^6 cells
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
  • Western Blot 1:5000-1:10000
• Application Notes: This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry. Use in ICC/IF was reported in scientific literature (PMID: 28001364). Use in functional reported in scientific literature (PMID:31358662).
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime molds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759). C. elegans reactivity reported in scientific literature (PMID: 29118344). Invertebrate reactivity reported in scientific literature (PMID:31857067).Other species have not been tested. Toxoplasma gondii reactivity reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for alpha Tubulin Antibody (YL1/2) - BSA Free

• alpha Tubulin
• Alpha-Tubulin 3
• B-ALPHA-1
• FLJ25113
• hum-a-tub1
• hum-a-tub2
• LIS3
• TUBA1A
• TUBA3
• tublin
• tubulin alpha-1A chain
• Tubulin alpha-3 chain
• Tubulin B-Alpha-1
• tubulin, alpha 1a
• tubulin, alpha 3
• tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha Tubulin Antibody (NB600-506)(45)

Publications using NB600-506

• Courtney A, Liegey J, Burke N Et al. Characterization of geometric variance in the epithelial nerve net of the ctenophore Pleurobrachia pileus J Comp Neurol 2021-12-21 [PMID: 34933399]
  
  Details:
  Citation using the DyLight 488 version of this antibody.
• Huang JW, Taglialatela A, Acharya A, Leuzzi G Identification of MCM8IP, an interactor of MCM8-9 and RPA1 that promotes homologous recombination and DNA synthesis in response to DNA damage bioRxiv 2019-09-03 (WB, Human)
• Thompson B, Sharp C, Dry I et al. Cellular Protein TTC4 and its Cofactor HSP90 are Pro-Viral for Bovine Herpesvirus 1 SSRN Electronic Journal 2022-08-10 [PMID: 36100007] (WB, Human)
  
  Details:
  Dilutions used for WB 1:1000 or 1:5000
• Diebold O, Gonzalez V, Venditti L et al. Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes Journal of virology 2022-07-27 [PMID: 35758692] (WB)
• Taglialatela A, Leuzzi G, Sannino V et al. REV1-Pol zeta maintains the viability of homologous recombination-deficient cancer cells through mutagenic repair of PRIMPOL-dependent ssDNA gaps Molecular cell 2021-09-07 [PMID: 34508659] (WB, Human)
• Cheng A, Jiang Y, Wang T et al. Energy restriction causes metaphase delay and chromosome mis-segregation in cancer cells Cell cycle (Georgetown, Tex.) 2021-05-28 [PMID: 34048314]
• Little JN, McNeely KC, Michel N et al. Loss of coiled-coil protein Cep55 impairs neural stem cell abscission and results in p53-dependent apoptosis in developing cortex The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-02-22 [PMID: 33622776]
• Mukhtar, E, Worroll, D Et al. Quantitative analysis of taxane drug target engagement of microtubules in circulating tumor cells from metastatic castration resistant prostate cancer patients treated with CRXL301, a nanoparticle of docetaxel. Cancer Drug Resist 2020-10-17 [PMID: 33062959] (WB, Human)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (WB, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• van Vijven M, van Groningen B, Kimenai JN et al. Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome - a diagnostic in vitro tissue remodeling platform J Exp Orthop 2020-07-04 [PMID: 32623555] (WB, Human)
• Huang JW, Acharya A, Taglialatela A et al. MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage Nat Commun 2020-06-11 [PMID: 32528060] (ICC/IF, WB, Human)
• Miyamoto T, Hosoba K, Itabashi T et al. Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome EMBO J. 2020-05-05 [PMID: 32368833] (ICC/IF)
• Courtney A, Alvey LM, Merces GOT et al. The Flexiscope: a low cost, flexible, convertible and modular microscope with automated scanning and micromanipulation R Soc Open Sci 2020-03-01 [PMID: 32269809] (ICC/IF)
• Kim Jh, Bang Ih, Noh Yj et Al. Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression Int J Mol Sci 2020-03-23 [PMID: 32210068] (WB, C. elegans)
• Heizer PJ, Yang Y, Tu Y et al. Deficiency in ZMPSTE24 and resulting farnesyl-prelamin A accumulation only modestly affect mouse adipose tissue stores J. Lipid Res. 2020-01-15 [PMID: 31941672] (WB, Mouse)
• Matsuo M, Onuma TA, Omotezako T, Nishida H Protein phosphatase 2A is essential to maintain meiotic arrest, and to prevent Ca2+ burst at spawning and eventual parthenogenesis in the larvacean Oikopleura dioica Dev. Biol. 2019-12-16 [PMID: 31857067] (IHC, Invertebrate)
• Guo Y, Gabola M, Lattanzio R et al. Loss of cyclin A2 in murine colonic epithelial cells disrupts colon homeostasis by triggering DNA damage and dysplasia and high cyclin A2 expression is a good-prognosis factor in patients with colorectal cancer bioRxiv (ICC/IF, Mouse)
• Oddoux S, Randazzo D, Kenea A et al. Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers Front Cell Dev Biol 2019-09-18 [PMID: 31620435] (ICC/IF, WB, Mouse)
• Nambiar TS, Billon P, Diedenhofen G et al. Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant Nat Commun 2019-07-30 [PMID: 31363085] (WB, Human)
• Zheng L, Chen Z, Kawakami M et al. Threonine Tyrosine Kinase Inhibition Eliminates Lung Cancers by Augmenting Apoptosis and Polyploidy Mol. Cancer Ther. 2019-07-29 [PMID: 31358662] (Func, Human, Mouse)
• Patterson JC, Joughin BA, Prota AE et al. VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells Cell Syst 2019-07-24 [PMID: 31302152]
• Ha S, Jeong SH, Yi K et al. Krt5+/Krt15+ foregut basal progenitors give rise to cyclooxygenase-2-dependent tumours in response to gastric acid stress Nat Commun 2019-05-20 [PMID: 31110179] (WB, Mouse)
• Dabral S, Muecke C, Valasarajan C et al. The conservation and functionality of the oxygen-sensing enzyme Factor Inhibiting HIF (FIH) in non-vertebrates PLoS ONE 2019-04-29 [PMID: 31034531] (WB, Human)
• Kittipassorn T, Haydinger CD, Wood JPM et al. Characterization of the novel spontaneously immortalized rat Muller cell line SIRMu-1. Exp. Eye Res. 2019-01-21 [PMID: 30677389] (WB, Rat)
• Colburn ZT, Jones JCR. Complexes of a6B4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration. J. Cell. Sci. 2018-07-30 [PMID: 29976561] (ICC/IF, Human)
• Bastin Donald, Aitken Amelia S, Pelin Adrian et al. Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference. J Immunother Cancer 2018-01-01 [PMID: 29921327] (WB, Mouse)
• Perni M, Aprile FA, Casford S et al. Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles Sci Rep. 2017-11-08 [PMID: 29118344] (ICC/IF, C. elegans)
• Miyamoto T, Akutsu SN, Fukumitsu A et al. PLK1-mediated phosphorylation of WDR62/MCPH2 ensures proper mitotic spindle orientation. Hum. Mol. Genet. 2017-08-26 [PMID: 28973348] (ICC/IF, Human)
• Banerjee SR, Foss CA, Horhota A et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues Biomacromolecules. 2017-01-09 [PMID: 28001364] (ICC/IF, Mouse)
  
  Details:
  Citation used the PE format of this antibody.
• Comaills V, Kabeche L et al. Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition. Cell Rep 2016-06-12 [PMID: 27926867] (ICC/IF, Human, Mouse)
• Hu S, Lu Y, Orr B et al. Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Mol. Cancer Ther. 2015-08-24 [PMID: 26304236]
• Giron-Martinez A, Perez-Rial S, Terron-Exposito R et al. Proliferative Activity of Liver Growth Factor is Associated with an Improvement of Cigarette Smoke-Induced Emphysema in Mice. PLoS OnE. 2014-11-18 [PMID: 25401951] (WB, Mouse)
• Ganem N J, Cornils H et al. Cytokinesis failure triggers hippo tumor suppressor pathway activation. Cell 2014-08-14 [PMID: 25126788] (WB, Human)
• Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013-09-01 [PMID: 24019759] (WB, Drosophila)
• Sung M, Giannakakou P. BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling Oncogene 2013-03-25 [PMID: 23524581] (ICC/IF, WB, Human)
• Batista-Nascimento L, Toledano MB, Thiele DJ et al. Yeast protective response to arsenate involves the repression of the high affinity iron uptake system Biochim Biophys Acta 2013-01-04 [PMID: 23295455] (WB, Human, Mouse)
• Pilling Darrell, Fan Ted, Huang Donna et al. Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts. PloS One 2009-01-01 [PMID: 19834619] (IHC, Mouse)
• O'Connell CB, Loncarek J, Kalab P et al. Relative contributions of chromatin and kinetochores to mitotic spindle assembly. J Cell Biol;187(1):43-51. 2009-01-01 [PMID: 19805628] (ICC/IF, Rat)
• Yang Z, Kenny AE, Brito DA, Rieder CL. Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments. J Cell Biol 2009-09-07 [PMID: 19720871] (ICC/IF, Human)
• Ganem N J, Godinho S A et al. A mechanism linking extra centrosomes to chromosomal instability. Nature 2009-09-07 [PMID: 19506557] (Rat)
• Bhattacharya R, Gonzalez AM, Debiase PJ et al. Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength. J Cell Sci 2009-05-01 [PMID: 19366731] (WB, Rat)
• Ohradanova A, Gradin K et al. Hypoxia upregulates expression of human endosialin gene via hypoxia-inducible factor 2. Br J Cancer 2008-10-21 [PMID: 18813310] (WB, Human)
• Liang, X-J et al. Endocytic recyling compartments altered in cisplatin-resistant cancer cells. Cancer Res 66: 2346-2353. 2006-01-01 [PMID: 16489040] (ICC/IF, WB, Human)
• Iida, S et al. Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression G1 tetraploidy checkpoint function. Oncogene 23: 5266-5274. 2004-01-01 [PMID: 15122335] (WB, Rat)
• 1 Allen JD et al. Extensive contribution of the multidrug transporters P-glycoprotein Mrp1 to basal drug resistance. Cancer Res, 60: 5761-6. 2000-01-01 [PMID: 11059771] (WB, Mouse)

Reviews for alpha Tubulin Antibody (NB600-506) (4)

Average Rating: 5

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Toxoplasma gondii.

reviewed by: Verified Customer WB Toxoplasma gondii 05/22/2019

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates
• Species: Toxoplasma gondii
• Lot: E-1

Comments

• Comments: Western Blot analysis from T. gondii tachyzoites whole cell lysates. This antibody was used at 1:10000 dilution.

reviewed by: Verified Customer ICC Human 06/11/2014

Summary

• Application: Immunocytochemistry
• Species: Human

reviewed by: Verified Customer WB Human 06/11/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: Verified Customer IF 04/28/2014

Summary

• Application: Immunofluorescence

FAQs for alpha Tubulin Antibody (NB600-506). (Showing 1 - 2 of 2 FAQs).

1. Will this alpha tubulin antibody recognize both isoforms of alpha tubulin? 
   • The epitope for this alpha tubulin antibody lies on the C-terminus of the protein and the difference between the two major isoforms is within the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.
2. We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?
   • Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

Bioinformatics

• Gene Symbol: TUBA1A