alpha Tubulin Antibody (YL1/2)

Images

 
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 ...read more
Immunohistochemistry-Paraffin: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Flow Cytometry: Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of Tubulin expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - WB analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as ...read more
Flow Cytometry: Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a ...read more
Flow Cytometry: Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ce, Dr, Ma, Mk, Pm, YeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clone
YL1/2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

alpha Tubulin Antibody (YL1/2) Summary

Immunogen
Full length native protein (purified) (S. cerevisiae).
Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Marker
Microtubule Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • ELISA 1:100-1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-506 in the following applications:

Publications
Read Publications using
NB600-506 in the following applications:

  • 7 publications
  • IF
    1 publication
  • IHC
    1 publication
  • WB
    11 publications

Reactivity Notes

Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime molds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759). C. elegans reactivity reported in scientific literature (PMID: 29118344). Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for alpha Tubulin Antibody (YL1/2)

  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA1A
  • TUBA3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin alpha-1A chain (TUBA1A) is a member of the tubulin family, which are the primary constituents of microtubules. Tubulin binds to GTP in two locations, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. The TUBA1A gene is one of three alpha tubulin genes located on chromosome 12q. TUBA1A is found on the cytoskeleton and is expressed predominantly in morphologically differentiated neurologic cells. Mutations to the TUBA1A gene causes a neurological condition called lissencephaly type 3 (LIS3). As a result of defective neuronal migration, LIS3 is characterized by microcephaly, mental retardation, and early-onset epilepsy (PMID: 22264709). The tubulin clone (YL1/2) specifically binds tyrosylated Tubulin (Tyr-Tubulin) (PMID: 6415068).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-506
Species: Hu, Mu, Rt, Av, Ce, Dr, Ma, Mk, Pm, Ye
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA

Publications for Tubulin Antibody (NB600-506)(21)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, C. elegans, Drosophila.

We have publications tested in 4 applications: ICC/IF, IF, IHC, WB.


Filter By Application
ICC/IF
(7)
IF
(1)
IHC
(1)
WB
(11)
All Applications
Filter By Species
Human
(8)
Mouse
(6)
Rat
(5)
C. elegans
(1)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB600-506 Applications Species
Kittipassorn T, Haydinger CD, Wood JPM et al. Characterization of the novel spontaneously immortalized rat Muller cell line SIRMu-1. Exp. Eye Res. Jan 21 2019 [PMID: 30677389] (WB, Rat) WB Rat
Colburn ZT, Jones JCR. Complexes of a6B4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration. J. Cell. Sci. Jul 30 2018 [PMID: 29976561] (ICC/IF, Human) ICC/IF Human
Bastin Donald, Aitken Amelia S, Pelin Adrian et al. Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference. J Immunother Cancer 2018 [PMID: 29921327] (WB, Mouse) WB Mouse
Perni M, Aprile FA, Casford S et al. Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles Sci Rep. 2017 Nov 08 [PMID: 29118344] (ICC/IF, C. elegans) ICC/IF C. elegans
Miyamoto T, Akutsu SN, Fukumitsu A et al. PLK1-mediated phosphorylation of WDR62/MCPH2 ensures proper mitotic spindle orientation. Hum. Mol. Genet. 2017 Aug 26 [PMID: 28973348] (ICC/IF, Human) ICC/IF Human
Valentine Comaills, Lilian Kabeche, Robert Morris, et al. Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition. Cell Reports 2016 Dec 06 [PMID: 27926867] (IF, Mouse, Human) IF Mouse, Human
Hu S, Lu Y, Orr B et al. Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Mol. Cancer Ther. 2015 Aug 24 [PMID: 26304236]
Giron-Martinez A, Perez-Rial S, Terron-Exposito R et al. Proliferative Activity of Liver Growth Factor is Associated with an Improvement of Cigarette Smoke-Induced Emphysema in Mice. PLoS OnE. 2014 Nov 18 [PMID: 25401951] (WB, Mouse) WB Mouse
Ganem NJ, Cornils H, Chiu SY et al. Cytokinesis failure triggers hippo tumor suppressor pathway activation. Cell. 2014 Aug 14 [PMID: 25126788] (WB, Human) WB Human
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013 Sep [PMID: 24019759] (WB, Drosophila) WB Drosophila
Show All 21 Publications.

Reviews for Tubulin Antibody (NB600-506) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB600-506:
Filter by Applications
ICC
(1)
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry alpha Tubulin NB600-506
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reviewed by:
Anony- mous
ICC Human 06/11/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot alpha Tubulin NB600-506
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reviewed by:
Anony- mous
WB Human 06/11/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS and NIH/3T3 cells using Tubulin antibody (NB600-506, 1:100).
FileView PDF
Immunofluorescence alpha Tubulin NB600-506
Enlarge
reviewed by:
Marissa Madden
IF 04/28/2014
View

Summary

ApplicationImmunofluorescence

Product General Protocols

View specific protocols for Tubulin Antibody (NB600-506): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB600-506). (Showing 1 - 6 of 6 FAQs).

  1. We are working with mouse liver tissue, will this alpha tubulin antibody work as a loading control?
    • Yes, alpha tubulin is expressed in nearly all tissue types including liver; however we do not recommend using as a loading control antibody in stomach or oral mucosa tissue as expression of alpha tubulin in these areas is minimal.
  2. Will your alpha tubulin antibody work as a loading control for simple western?
    • Yes, this alpha tubulin antibody has been validated in simple western and makes an excelent loading control antibody. Simple western has a lower detection limit of 12kDA and alpha tubulin detects around 55kDA.
  3. Will this alpha tubulin antibody recognize both isoforms of alpha tubulin?
    • The epitope for this alpha tubulin antiboyd lies on the C-terminus of the protein and the difference between the two major isoforms is witin the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.
  4. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
  5. We're looking at LC3B, aSYN and p62 which are 18, 23 and 65 kDA respectively so we need the loading control antibody to be between those.
    • Alpha tubulin antibody detects around 55kDA so that would be a good option for a loading control antibody for those targets as you should be able to easily distinguish from the other bands.
  6. I am looking for a primary alpha tubulin  antibody that is conjugated to a red fluorophore.
    • Okay, our most popular alpha tubulin antibody is NB100-690 and is available in a variety of formats. So if you are looking for red I would recommend a DyLight 550, 650 etc.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-506AF405
Alexa Fluor 488 Labeled NB600-506AF488
Alexa Fluor 647 Labeled NB600-506AF647
Alexa Fluor 700 Labeled NB600-506AF700
Allophycocyanin Labeled NB600-506APC
DyLight 350 Labeled NB600-506UV
DyLight 405 Labeled NB600-506V
DyLight 488 Labeled NB600-506G
DyLight 680 Labeled NB600-506FR
DyLight 755 Labeled NB600-506IR
FITC Labeled NB600-506F
PE Labeled NB600-506PE

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Bioinformatics Tool for Tubulin Antibody (NB600-506)

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Blogs on alpha Tubulin.

Visualizing Autophagy by Microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to vi...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Tubulins are the microtubule building block and exist as globular dimeric proteins of alpha/beta chains....  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anony- mous
06/11/2014
Application: ICC
Species: Human

Anony- mous
06/11/2014
Application: WB
Species: Human

Marissa Madden
04/28/2014
Application: IF
Species:

Bioinformatics

Gene Symbol TUBA1A