TRPM7 [p Ser1569] Antibody

Western Blot: TRPM7 [p Ser1569] Antibody [NBP3-23192] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRPM7 [p Ser1569] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-TRPM7 (Ser-1569) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1569 in human TRPM7. This site is well conserved in rat and mouse TRPM7, and is conserved in TRPM6 (Thr-1724) however the site is threonine instead of serine. This site has no homology to other TRPM family members. [UniProt# Q96QT4]
• Modification: p Ser1569
• Specificity: The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1569) then affinity purified using phospho-TRPM7 (Ser-1569) peptide. This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 stimulated with calyculin A and rat PC12 cells, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for TRPM7 [p Ser1569] Antibody

• CHAK
• CHAK1FLJ25718
• Channel-kinase 1
• EC 2.7.11.1
• Long transient receptor potential channel 7
• LTRPC ion channel family member 7
• LTrpC7
• LTrpC-7
• LTRPC7FLJ20117
• transient receptor potential cation channel subfamily M member 7
• transient receptor potential cation channel, subfamily M, member 7
• transient receptor potential-phospholipase C-interacting kinase
• TRP-PLIK

Background

The TRPM7 (Transient Receptor Protein-PhosphoLipase C-Interacting Kinase) protein is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. TRPM7, however, is unique among the TRP channel proteins because it is bi-functional. It not only controls ion channels but it also acts as a kinase by phosphorylating proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM7