Annexin A1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat ...read more
Annexin A1/Annexin I was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse/Rat Annexin A1/Annexin I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) at 10 ...read more
Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Annexin A1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Annexin A1
Met1-Asn346
Accession # P04083
Specificity
The antibody detects human, mouse and rat Annexin A1 in Western blots. In Western blots, goat anti‑human/mouse/rat Annexin A1 does not cross‑react with recombinant human Annexin A2, A3, A4, A6, A7, A8, A9, A10, A11 or A13.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANXA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF3770 in the following applications:

Publications
Read Publication using
AF3770 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A1 Antibody [Unconjugated]

  • Annexin A1
  • annexin I (lipocortin I)
  • Annexin I
  • Annexin-1
  • ANX1
  • ANXA1
  • Calpactin II
  • calpactin-2
  • chromobindin-9
  • Lipocortin I
  • LPC1
  • LPC1annexin-1
  • p35
  • Phospholipase A2 inhibitory protein

Background

The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% identity with mouse and rat Annexin A1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin A1 Antibody (AF3770)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
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Review for Annexin A1 Antibody (AF3770) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using AF3770:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20618702
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20618702

Details

Detection NotesSee PMID 20618702

Comments

CommentsPublished: See PMID 20618702Independent review from antibodyresource.com

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Recent Reviews

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4
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1
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Anonymous
01/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ANXA1
Entrez
Uniprot