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TRPC1 Antibody

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Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained ...read more
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in ...read more
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat olfactory bulb at 1:500 dilution using Rabbit antibody to TRPC1: whole serum, DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in ...read more
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in ...read more
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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TRPC1 Antibody Summary

Immunogen
A synthetic peptide from human TRPC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPC1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:1000
  • Western Blot 1:1000
Publications
Read Publications using
NB100-98844 in the following applications:

  • IHC-P
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TRPC1 Antibody

  • HTRP-1
  • MGC133334
  • MGC133335
  • transient receptor potential canonical 1
  • transient receptor potential cation channel, subfamily C, member 1
  • transient receptor potential channel 1
  • Transient receptor protein 1
  • TRP1
  • TRP-1
  • TRP1short transient receptor potential channel 1
  • TRPC1

Background

FUNCTION: Thought to form a receptor-activated non-selective calcium permanent cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. SUBUNIT: Interacts with TRPC3. Interacts with ITPR3. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Seems to be ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:500.
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Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody ) at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.
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Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.
Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.
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Recombinant Human
DLEU2 GST (N-Term) Pr ...
H00008847-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, WB
TRPC1 Antibody
NB100-98844
TRPC1 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for TRPC1 Antibody (NB100-98844)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
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(2)
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Product General Protocols

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FAQs for TRPC1 Antibody (NB100-98844). (Showing 1 - 1 of 1 FAQs).

  1. I am very interested in your anti-TRPC1 antibody NB100-98844. I would like to know the epitope of this antibody before purchasing. I did not find this information on the website. Can you please provide me with the information?
    • Our TRP1 antibody with catalogue number NB100-98844 was raised to a synthetic peptide derived from the second extracellular loop (within amino acids 500-540) of the human protein (Please see Uniprot Site) conjugated to an immunogenic carrier protein. As we do not routinely epitope map our antibodies I am unable to provide any additional information regarding the exact binding of NB100-98844 to TRP1.

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Bioinformatics

Gene Symbol TRPC1
Entrez
Uniprot