Western Blot: VRL1 Antibody [NBP1-32096] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE VRL1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the ...read more
Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-32096] - 293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag. 293T cells transfected with ...read more
Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-32096] - Analysis of paraffin-embedded human lung, using VRL1 antibody(10 ug/ml).
Western Blot: VRL1 Antibody [NBP1-32096] - Sample(30 ug of whole cell lysate) A:A431 B:Raji 7.5% SDS PAGE diluted at 1:500
VRL1 antibody validated for ICC/IF from a verified customer review.
86 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-32096 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Immunogen affinity purified
Alternate Names for VRL1 Antibody
Osm-9-like TRP channel 2
transient receptor potential cation channel, subfamily V, member 2
Vanilloid receptor-like protein 1
VRL1transient receptor potential cation channel subfamily V member 2
This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer. Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome. Note: Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for VRL1 Antibody (NBP1-32096)(1)
We have publications tested in 1 confirmed species: Human.
293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag.293T cells transfected with vector, pCMV5 3xFlag-TRPV2, as experimental group. TRPV2 was co-localized with Flag at cell membrane.ICCFlag: greenTRPV2: redNucleus: bluemCherry: red fluorescent protein
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