Western Blot: VRL1 Antibody [NBP1-32096] - Sample(30 ug of whole cell lysate) A:A431 B:Raji 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-32096] - 293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag. 293T cells transfected with ...read more
86 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-32096 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Immunogen affinity purified
Alternate Names for VRL1 Antibody
Osm-9-like TRP channel 2
transient receptor potential cation channel, subfamily V, member 2
Vanilloid receptor-like protein 1
VRL1transient receptor potential cation channel subfamily V member 2
This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer. Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome. Note: Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag.293T cells transfected with vector, pCMV5 3xFlag-TRPV2, as experimental group. TRPV2 was co-localized with Flag at cell membrane.ICCFlag: greenTRPV2: redNucleus: bluemCherry: red fluorescent protein
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