VRL1 Antibody

Images

 
Western Blot: VRL1 Antibody [NBP1-32096] - Sample(30 ug of whole cell lysate) A:A431 B:Raji 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-32096] - 293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag. 293T cells transfected with ...read more
Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-32096] - Paraffin-embedded Lung, using antibody 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.71 mg/ml

Order Details

VRL1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VRL1. The exact sequence is proprietary.
Localization
Cell membrane, Multi-pass membrane protein, Cytoplasm, Melanosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPV2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunocytochemistry
Application Notes
ICC usage reported by customer review.
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32096 in the following applications:

Publications
Read Publication using
NBP1-32096 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.71 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VRL1 Antibody

  • Osm-9-like TRP channel 2
  • transient receptor potential cation channel, subfamily V, member 2
  • TrpV2
  • Vanilloid receptor-like protein 1
  • VRL-1MGC12549
  • VRL1transient receptor potential cation channel subfamily V member 2
  • VRLOTRPC2

Background

This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer. Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome. Note: Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-32096
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Applications: WB, ICC/IF, IHC, IHC-P, ICC

Publications for VRL1 Antibody (NBP1-32096)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
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Review for VRL1 Antibody (NBP1-32096) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-32096:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry VRL1 NBP1-32096
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reviewed by:
Anony- mous
ICC Human 11/07/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special Applications293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag.293T cells transfected with vector, pCMV5 3xFlag-TRPV2, as experimental group. TRPV2 was co-localized with Flag at cell membrane.ICCFlag: greenTRPV2: redNucleus: bluemCherry: red fluorescent protein
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Video Protocols

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ICC/IF Video Protocol

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Anony- mous
11/07/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TRPV2